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Poly(Methyl Methacrylate) as a matrix for immobilization of lipase

Poly(methyl methacrylate) (PMMA) was found to be suitable for the immobilization of lipase from Candida rugosa. The best result based on hydrolytic activity was obtained by adsorption of the purified unbuffered enzyme solution onto PMMA beads without any modification of the beads. Prolonged exposure...

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Main Authors: Yunus, W. M. Z. Wan, Salleh, A. B., Ismail, A., Ampon, K., Razak, C. N. A., Basri, M.
Format: Article
Language:English
Published: Humana Press 1992
Online Access:http://psasir.upm.edu.my/id/eprint/114984/1/114984.pdf
http://psasir.upm.edu.my/id/eprint/114984/
https://link.springer.com/article/10.1007/BF02929689?error=cookies_not_supported&code=d0f214c7-9ad4-453d-8977-aa0b0b396090
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spelling my.upm.eprints.1149842025-02-13T06:50:08Z http://psasir.upm.edu.my/id/eprint/114984/ Poly(Methyl Methacrylate) as a matrix for immobilization of lipase Yunus, W. M. Z. Wan Salleh, A. B. Ismail, A. Ampon, K. Razak, C. N. A. Basri, M. Poly(methyl methacrylate) (PMMA) was found to be suitable for the immobilization of lipase from Candida rugosa. The best result based on hydrolytic activity was obtained by adsorption of the purified unbuffered enzyme solution onto PMMA beads without any modification of the beads. Prolonged exposure of the protein to the beads increased its adsorption but the expressed activity decreased after 1 h of exposure. The magnitude of the immobilized activity also varied with the size of the beads. Immobilization of the lipase shifted its optimal reaction temperature from 37 to 45°C. The immobilized enzyme is also more stable than the free enzyme in solution. The operational half-life of the immobilized lipase packed in a column and assayed in a closed system is 40 d. Humana Press 1992-08 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/114984/1/114984.pdf Yunus, W. M. Z. Wan and Salleh, A. B. and Ismail, A. and Ampon, K. and Razak, C. N. A. and Basri, M. (1992) Poly(Methyl Methacrylate) as a matrix for immobilization of lipase. Applied Biochemistry and Biotechnology, 36 (2). pp. 97-105. ISSN 1559-0291; eISSN: 0273-2289 https://link.springer.com/article/10.1007/BF02929689?error=cookies_not_supported&code=d0f214c7-9ad4-453d-8977-aa0b0b396090 10.1007/BF02929689
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Poly(methyl methacrylate) (PMMA) was found to be suitable for the immobilization of lipase from Candida rugosa. The best result based on hydrolytic activity was obtained by adsorption of the purified unbuffered enzyme solution onto PMMA beads without any modification of the beads. Prolonged exposure of the protein to the beads increased its adsorption but the expressed activity decreased after 1 h of exposure. The magnitude of the immobilized activity also varied with the size of the beads. Immobilization of the lipase shifted its optimal reaction temperature from 37 to 45°C. The immobilized enzyme is also more stable than the free enzyme in solution. The operational half-life of the immobilized lipase packed in a column and assayed in a closed system is 40 d.
format Article
author Yunus, W. M. Z. Wan
Salleh, A. B.
Ismail, A.
Ampon, K.
Razak, C. N. A.
Basri, M.
spellingShingle Yunus, W. M. Z. Wan
Salleh, A. B.
Ismail, A.
Ampon, K.
Razak, C. N. A.
Basri, M.
Poly(Methyl Methacrylate) as a matrix for immobilization of lipase
author_facet Yunus, W. M. Z. Wan
Salleh, A. B.
Ismail, A.
Ampon, K.
Razak, C. N. A.
Basri, M.
author_sort Yunus, W. M. Z. Wan
title Poly(Methyl Methacrylate) as a matrix for immobilization of lipase
title_short Poly(Methyl Methacrylate) as a matrix for immobilization of lipase
title_full Poly(Methyl Methacrylate) as a matrix for immobilization of lipase
title_fullStr Poly(Methyl Methacrylate) as a matrix for immobilization of lipase
title_full_unstemmed Poly(Methyl Methacrylate) as a matrix for immobilization of lipase
title_sort poly(methyl methacrylate) as a matrix for immobilization of lipase
publisher Humana Press
publishDate 1992
url http://psasir.upm.edu.my/id/eprint/114984/1/114984.pdf
http://psasir.upm.edu.my/id/eprint/114984/
https://link.springer.com/article/10.1007/BF02929689?error=cookies_not_supported&code=d0f214c7-9ad4-453d-8977-aa0b0b396090
_version_ 1825162409590915072
score 13.239859

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