Sugar esterification catalysed by alkylated trypsin in dimethylformamide

Reductive alkylation of porcine pancreatic trypsin with acetaldehyde, propionaldehyde, octaldehyde and benzaldehyde resulted in about 5 to 6 folds increase in the sugar esterification activities of the enzyme in DMF. The optimum activities of the modified enzymes depend on the degrees of their modif...

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Main Authors: Ampon, K., Salleh, A.B., Teoh, A., Wan Yunus, W.M.Z.
Format: Article
Language:English
Published: Kluwer Academic Publishers 1991
Online Access:http://psasir.upm.edu.my/id/eprint/114788/1/114788.pdf
http://psasir.upm.edu.my/id/eprint/114788/
https://link.springer.com/article/10.1007/BF01033511?error=cookies_not_supported&code=d314132a-177e-48a4-8f48-a76a7bc74246
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spelling my.upm.eprints.1147882025-01-31T03:58:53Z http://psasir.upm.edu.my/id/eprint/114788/ Sugar esterification catalysed by alkylated trypsin in dimethylformamide Ampon, K. Salleh, A.B. Teoh, A. Wan Yunus, W.M.Z. Reductive alkylation of porcine pancreatic trypsin with acetaldehyde, propionaldehyde, octaldehyde and benzaldehyde resulted in about 5 to 6 folds increase in the sugar esterification activities of the enzyme in DMF. The optimum activities of the modified enzymes depend on the degrees of their modification with the respective aldehydes. These alkylated trypsins were more stable in DMF compared to the native unmodified enzyme at temperatures between 26 and 60°C. © 1991 Kluwer Academic Publishers. Kluwer Academic Publishers 1991 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/114788/1/114788.pdf Ampon, K. and Salleh, A.B. and Teoh, A. and Wan Yunus, W.M.Z. (1991) Sugar esterification catalysed by alkylated trypsin in dimethylformamide. Biotechnology Letters, 13 (1). pp. 25-30. ISSN 1573-6776; eISSN: 0141-5492 https://link.springer.com/article/10.1007/BF01033511?error=cookies_not_supported&code=d314132a-177e-48a4-8f48-a76a7bc74246 10.1007/BF01033511
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Reductive alkylation of porcine pancreatic trypsin with acetaldehyde, propionaldehyde, octaldehyde and benzaldehyde resulted in about 5 to 6 folds increase in the sugar esterification activities of the enzyme in DMF. The optimum activities of the modified enzymes depend on the degrees of their modification with the respective aldehydes. These alkylated trypsins were more stable in DMF compared to the native unmodified enzyme at temperatures between 26 and 60°C. © 1991 Kluwer Academic Publishers.
format Article
author Ampon, K.
Salleh, A.B.
Teoh, A.
Wan Yunus, W.M.Z.
spellingShingle Ampon, K.
Salleh, A.B.
Teoh, A.
Wan Yunus, W.M.Z.
Sugar esterification catalysed by alkylated trypsin in dimethylformamide
author_facet Ampon, K.
Salleh, A.B.
Teoh, A.
Wan Yunus, W.M.Z.
author_sort Ampon, K.
title Sugar esterification catalysed by alkylated trypsin in dimethylformamide
title_short Sugar esterification catalysed by alkylated trypsin in dimethylformamide
title_full Sugar esterification catalysed by alkylated trypsin in dimethylformamide
title_fullStr Sugar esterification catalysed by alkylated trypsin in dimethylformamide
title_full_unstemmed Sugar esterification catalysed by alkylated trypsin in dimethylformamide
title_sort sugar esterification catalysed by alkylated trypsin in dimethylformamide
publisher Kluwer Academic Publishers
publishDate 1991
url http://psasir.upm.edu.my/id/eprint/114788/1/114788.pdf
http://psasir.upm.edu.my/id/eprint/114788/
https://link.springer.com/article/10.1007/BF01033511?error=cookies_not_supported&code=d314132a-177e-48a4-8f48-a76a7bc74246
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score 13.239859