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Anti-atherogenic effect of Channa striatus fish extract in high cholesterol-fed rabbits

Purpose: To determine the effect of aqueous extract of Channa striatus on plasma lipids concentrations, pro-inflammatory cytokines levels, adhesion molecules and arterial plaque formation in cholesterol-fed rabbits. Methods: New Zealand rabbits (n = 30, females) were divided into five groups of six...

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Main Authors: Hakim, Muhammad Nazrul, Yee, Pang Chong, Cheema, Manraj Singh, Keong, Yong Yoke, Ahmad, Zuraini
Format: Article
Language:English
Published: University of Benin 2024
Online Access:http://psasir.upm.edu.my/id/eprint/114718/1/114718.pdf
http://psasir.upm.edu.my/id/eprint/114718/
https://www.ajol.info/index.php/tjpr/article/view/278008
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spelling my.upm.eprints.1147182025-01-24T04:11:21Z http://psasir.upm.edu.my/id/eprint/114718/ Anti-atherogenic effect of Channa striatus fish extract in high cholesterol-fed rabbits Hakim, Muhammad Nazrul Yee, Pang Chong Cheema, Manraj Singh Keong, Yong Yoke Ahmad, Zuraini Purpose: To determine the effect of aqueous extract of Channa striatus on plasma lipids concentrations, pro-inflammatory cytokines levels, adhesion molecules and arterial plaque formation in cholesterol-fed rabbits. Methods: New Zealand rabbits (n = 30, females) were divided into five groups of six rabbits each as follows: cholesterol control group (0.5 % cholesterol); normal control group (normal diet), positive control group (0.5 % cholesterol + 5 mg/kg atorvastatin); and treatment groups (0.5% cholesterol + 250 mg/kg or 500 mg/kg C. striatus extracts, respectively) for 6 weeks. Blood was taken every 2 weeks and analyzed for total cholesterol (TC), low-density lipoprotein cholesterol (LDL), high-density lipoprotein cholesterol (HDL), triglyceride (TG), tumor necrosis factor-alpha (TNF-α) and interleukin-1 beta (IL-1β). Atherogenic index (AI) was calculated based on LDL:HDL ratio. Results: Cholesterol feeding for 6 weeks resulted in significantly increased TC, LDL, HDL, AI, TG, TNF-α and IL-1β when compared to C. striatus-fed rabbits. Serum LDL was 26.34 ± 1.43 mmol/L in C. striatus group to 30.52 ± 0.82 mmol/L in cholesterol group (p < 0.05). C. striatus extract (250 mg/kg) increased HDL by approximately 400 % (6.23 ± 1.76 mmol/L from 0.72 ± 0.18 mmol/L), decreased AI value (4.6 in C. striatus group to 10.4 in cholesterol group). Also, IL-1β and TNF-α concentration also significantly (p < 0.05) reduced with C. striatus administration. Interestingly, down-regulation of ICAM-1 and VCAM-1 expression and reduced plaque formation were observed with C. striatus feeding. Conclusion: Channa striatus extract is effective in reducing atherogenesis. This could be due to the high HDL level produced in 250 mg/kg dose group, and strongly suggests the significance of HDL in preventing atherogenesis even if other lipid profiles remain unchanged. University of Benin 2024-09-06 Article PeerReviewed text en cc_by_4 http://psasir.upm.edu.my/id/eprint/114718/1/114718.pdf Hakim, Muhammad Nazrul and Yee, Pang Chong and Cheema, Manraj Singh and Keong, Yong Yoke and Ahmad, Zuraini (2024) Anti-atherogenic effect of Channa striatus fish extract in high cholesterol-fed rabbits. Tropical Journal of Pharmaceutical Research, 23 (7). pp. 1083-1092. ISSN 1596-5996; eISSN: 1596-9827 https://www.ajol.info/index.php/tjpr/article/view/278008 10.4314/tjpr.v23i7.6
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Purpose: To determine the effect of aqueous extract of Channa striatus on plasma lipids concentrations, pro-inflammatory cytokines levels, adhesion molecules and arterial plaque formation in cholesterol-fed rabbits. Methods: New Zealand rabbits (n = 30, females) were divided into five groups of six rabbits each as follows: cholesterol control group (0.5 % cholesterol); normal control group (normal diet), positive control group (0.5 % cholesterol + 5 mg/kg atorvastatin); and treatment groups (0.5% cholesterol + 250 mg/kg or 500 mg/kg C. striatus extracts, respectively) for 6 weeks. Blood was taken every 2 weeks and analyzed for total cholesterol (TC), low-density lipoprotein cholesterol (LDL), high-density lipoprotein cholesterol (HDL), triglyceride (TG), tumor necrosis factor-alpha (TNF-α) and interleukin-1 beta (IL-1β). Atherogenic index (AI) was calculated based on LDL:HDL ratio. Results: Cholesterol feeding for 6 weeks resulted in significantly increased TC, LDL, HDL, AI, TG, TNF-α and IL-1β when compared to C. striatus-fed rabbits. Serum LDL was 26.34 ± 1.43 mmol/L in C. striatus group to 30.52 ± 0.82 mmol/L in cholesterol group (p < 0.05). C. striatus extract (250 mg/kg) increased HDL by approximately 400 % (6.23 ± 1.76 mmol/L from 0.72 ± 0.18 mmol/L), decreased AI value (4.6 in C. striatus group to 10.4 in cholesterol group). Also, IL-1β and TNF-α concentration also significantly (p < 0.05) reduced with C. striatus administration. Interestingly, down-regulation of ICAM-1 and VCAM-1 expression and reduced plaque formation were observed with C. striatus feeding. Conclusion: Channa striatus extract is effective in reducing atherogenesis. This could be due to the high HDL level produced in 250 mg/kg dose group, and strongly suggests the significance of HDL in preventing atherogenesis even if other lipid profiles remain unchanged.
format Article
author Hakim, Muhammad Nazrul
Yee, Pang Chong
Cheema, Manraj Singh
Keong, Yong Yoke
Ahmad, Zuraini
spellingShingle Hakim, Muhammad Nazrul
Yee, Pang Chong
Cheema, Manraj Singh
Keong, Yong Yoke
Ahmad, Zuraini
Anti-atherogenic effect of Channa striatus fish extract in high cholesterol-fed rabbits
author_facet Hakim, Muhammad Nazrul
Yee, Pang Chong
Cheema, Manraj Singh
Keong, Yong Yoke
Ahmad, Zuraini
author_sort Hakim, Muhammad Nazrul
title Anti-atherogenic effect of Channa striatus fish extract in high cholesterol-fed rabbits
title_short Anti-atherogenic effect of Channa striatus fish extract in high cholesterol-fed rabbits
title_full Anti-atherogenic effect of Channa striatus fish extract in high cholesterol-fed rabbits
title_fullStr Anti-atherogenic effect of Channa striatus fish extract in high cholesterol-fed rabbits
title_full_unstemmed Anti-atherogenic effect of Channa striatus fish extract in high cholesterol-fed rabbits
title_sort anti-atherogenic effect of channa striatus fish extract in high cholesterol-fed rabbits
publisher University of Benin
publishDate 2024
url http://psasir.upm.edu.my/id/eprint/114718/1/114718.pdf
http://psasir.upm.edu.my/id/eprint/114718/
https://www.ajol.info/index.php/tjpr/article/view/278008
_version_ 1823093250880176128
score 13.244413

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