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Mechanism of damnacanthal induced apoptosis in CEM-SS cell line

Leukaemia, is cancer of organs that is responsible to produce blood specifically the lymphatic system and bone marrow. Due to the harsh effects of currently used cancer drugs, damnacanthal, an anthraquinone obtained from the roots of Morinda elliptica is tested as a potential anticancer agent. This...

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Main Authors: Gopalsamy, Banulata, Saiful Yazan, Latifah, Abdul Hamid, Hisyam
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2024
Online Access:http://psasir.upm.edu.my/id/eprint/114674/1/114674.pdf
http://psasir.upm.edu.my/id/eprint/114674/
https://www.ukm.my/jsm/pdf_files/SM-PDF-53-9-2024/20.pdf
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spelling my.upm.eprints.1146742025-01-22T08:52:05Z http://psasir.upm.edu.my/id/eprint/114674/ Mechanism of damnacanthal induced apoptosis in CEM-SS cell line Gopalsamy, Banulata Saiful Yazan, Latifah Abdul Hamid, Hisyam Leukaemia, is cancer of organs that is responsible to produce blood specifically the lymphatic system and bone marrow. Due to the harsh effects of currently used cancer drugs, damnacanthal, an anthraquinone obtained from the roots of Morinda elliptica is tested as a potential anticancer agent. This study reports on the participation of the p53, Bcl-2 and Bax in the apoptosis induced by of damnacanthal, on T-lymphoblastic leukaemia (CEM-SS) cell. Cell viability and morphology was tested with trypan blue assay, flow cytometry analysis detected the apoptotic activity of damnacanthal, caspase colorimetric protease assay tested the Caspase 2, 3, 6, 8, and 9’s involvement and Enzyme-linked Immunosorbent Assay (ELISA) was carried out to quantify the Human p53, Bcl-2, and Bax expression levels. Damnacanthal exhibited cytotoxicity at doses 10 and 30 µg/mL after 72 h of incubation. This study reports that damnacanthal arrested the cell at G2/M phase and initiates the apoptotic activity in the cells treated with 30 µg/ mL of damnacanthal for 72 h through caspase 2 and 6 activation and not caspases 3, 8, and 9. Furthermore, this anthraquinone induces apoptosis via p53-independent pathway. Damnacanthal also lowered Bcl-2 and increased Bax activity in CEM-SS cell lines. These anticancer properties of damnacanthal makes it a potential agent to treat T-lymphoblastic leukaemia. © 2024 Penerbit Universiti Kebangsaan Malaysia. All rights reserved. Penerbit Universiti Kebangsaan Malaysia 2024 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/114674/1/114674.pdf Gopalsamy, Banulata and Saiful Yazan, Latifah and Abdul Hamid, Hisyam (2024) Mechanism of damnacanthal induced apoptosis in CEM-SS cell line. Sains Malaysiana, 53 (9). pp. 3159-3171. ISSN 0126-6039; eISSN: 0126-6039 https://www.ukm.my/jsm/pdf_files/SM-PDF-53-9-2024/20.pdf 10.17576/jsm-2024-5309-20
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Leukaemia, is cancer of organs that is responsible to produce blood specifically the lymphatic system and bone marrow. Due to the harsh effects of currently used cancer drugs, damnacanthal, an anthraquinone obtained from the roots of Morinda elliptica is tested as a potential anticancer agent. This study reports on the participation of the p53, Bcl-2 and Bax in the apoptosis induced by of damnacanthal, on T-lymphoblastic leukaemia (CEM-SS) cell. Cell viability and morphology was tested with trypan blue assay, flow cytometry analysis detected the apoptotic activity of damnacanthal, caspase colorimetric protease assay tested the Caspase 2, 3, 6, 8, and 9’s involvement and Enzyme-linked Immunosorbent Assay (ELISA) was carried out to quantify the Human p53, Bcl-2, and Bax expression levels. Damnacanthal exhibited cytotoxicity at doses 10 and 30 µg/mL after 72 h of incubation. This study reports that damnacanthal arrested the cell at G2/M phase and initiates the apoptotic activity in the cells treated with 30 µg/ mL of damnacanthal for 72 h through caspase 2 and 6 activation and not caspases 3, 8, and 9. Furthermore, this anthraquinone induces apoptosis via p53-independent pathway. Damnacanthal also lowered Bcl-2 and increased Bax activity in CEM-SS cell lines. These anticancer properties of damnacanthal makes it a potential agent to treat T-lymphoblastic leukaemia. © 2024 Penerbit Universiti Kebangsaan Malaysia. All rights reserved.
format Article
author Gopalsamy, Banulata
Saiful Yazan, Latifah
Abdul Hamid, Hisyam
spellingShingle Gopalsamy, Banulata
Saiful Yazan, Latifah
Abdul Hamid, Hisyam
Mechanism of damnacanthal induced apoptosis in CEM-SS cell line
author_facet Gopalsamy, Banulata
Saiful Yazan, Latifah
Abdul Hamid, Hisyam
author_sort Gopalsamy, Banulata
title Mechanism of damnacanthal induced apoptosis in CEM-SS cell line
title_short Mechanism of damnacanthal induced apoptosis in CEM-SS cell line
title_full Mechanism of damnacanthal induced apoptosis in CEM-SS cell line
title_fullStr Mechanism of damnacanthal induced apoptosis in CEM-SS cell line
title_full_unstemmed Mechanism of damnacanthal induced apoptosis in CEM-SS cell line
title_sort mechanism of damnacanthal induced apoptosis in cem-ss cell line
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2024
url http://psasir.upm.edu.my/id/eprint/114674/1/114674.pdf
http://psasir.upm.edu.my/id/eprint/114674/
https://www.ukm.my/jsm/pdf_files/SM-PDF-53-9-2024/20.pdf
_version_ 1823093234752028672
score 13.244413

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