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GSK-3β phosphorylation and alteration of β-catenin in hepatocellular carcinoma

The aim of this study is to investigate the potential correlation between the expression of phosphorylated glycogen synthase kinase-3β (phospho-GSK-3β) and β-catenin, and the mutations of β-catenin gene at the consensus GSK-3β phosphorylation site. The reason for this approach is to gain a better un...

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Main Authors: Ban, Ke Chen, Singh, Harjit, Krishnan, R., Seow, Heng Fong
Format: Article
Language:English
Published: Elsevier Ireland 2003
Online Access:http://psasir.upm.edu.my/id/eprint/114566/1/114566.pdf
http://psasir.upm.edu.my/id/eprint/114566/
https://linkinghub.elsevier.com/retrieve/pii/S030438350300421X
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spelling my.upm.eprints.1145662025-03-04T08:03:03Z http://psasir.upm.edu.my/id/eprint/114566/ GSK-3β phosphorylation and alteration of β-catenin in hepatocellular carcinoma Ban, Ke Chen Singh, Harjit Krishnan, R. Seow, Heng Fong The aim of this study is to investigate the potential correlation between the expression of phosphorylated glycogen synthase kinase-3β (phospho-GSK-3β) and β-catenin, and the mutations of β-catenin gene at the consensus GSK-3β phosphorylation site. The reason for this approach is to gain a better understanding of the molecular mechanisms of hepatocarcinogenesis in Malaysia. The expression of phospho-GSK-3β and β-catenin by immunohistochemistry and the mutations of β-catenin were studied in 23 hepatocellular carcinoma (HCC) and surrounding tissues. Overexpression of phospho-GSK-3β and β-catenin was found in 12/23 (52.2%) and 13/23 (56.5%) in HCC tissues, 6/23 (26.1%) and 9/23 (39.1%) in surrounding tissues, respectively. Overexpression of β-catenin in HCC tissues compared to the surrounding liver tissue was found to be higher in HCC tissues (p=0.015). In addition, we found that the expression of phospho-GSK-3β was related with the accumulation of β-catenin in surrounding tissues (p<0.05). The expression of phospho-GSK-3β and its association with the development of HCC is reported for the first time. In addition, this is the first report from Malaysia which shows that there are no mutations at the GSK-3β consensus phosphorylation sites on β-catenin gene in all 23 paired HCC and surrounding tissues. This result differed from HCC in geographical areas with high aflatoxin exposure. Elsevier Ireland 2003 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/114566/1/114566.pdf Ban, Ke Chen and Singh, Harjit and Krishnan, R. and Seow, Heng Fong (2003) GSK-3β phosphorylation and alteration of β-catenin in hepatocellular carcinoma. Cancer Letters, 199 (2). pp. 201-208. ISSN 0304-3835; eISSN: 0304-3835 https://linkinghub.elsevier.com/retrieve/pii/S030438350300421X 10.1016/S0304-3835(03)00421-X
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The aim of this study is to investigate the potential correlation between the expression of phosphorylated glycogen synthase kinase-3β (phospho-GSK-3β) and β-catenin, and the mutations of β-catenin gene at the consensus GSK-3β phosphorylation site. The reason for this approach is to gain a better understanding of the molecular mechanisms of hepatocarcinogenesis in Malaysia. The expression of phospho-GSK-3β and β-catenin by immunohistochemistry and the mutations of β-catenin were studied in 23 hepatocellular carcinoma (HCC) and surrounding tissues. Overexpression of phospho-GSK-3β and β-catenin was found in 12/23 (52.2%) and 13/23 (56.5%) in HCC tissues, 6/23 (26.1%) and 9/23 (39.1%) in surrounding tissues, respectively. Overexpression of β-catenin in HCC tissues compared to the surrounding liver tissue was found to be higher in HCC tissues (p=0.015). In addition, we found that the expression of phospho-GSK-3β was related with the accumulation of β-catenin in surrounding tissues (p<0.05). The expression of phospho-GSK-3β and its association with the development of HCC is reported for the first time. In addition, this is the first report from Malaysia which shows that there are no mutations at the GSK-3β consensus phosphorylation sites on β-catenin gene in all 23 paired HCC and surrounding tissues. This result differed from HCC in geographical areas with high aflatoxin exposure.
format Article
author Ban, Ke Chen
Singh, Harjit
Krishnan, R.
Seow, Heng Fong
spellingShingle Ban, Ke Chen
Singh, Harjit
Krishnan, R.
Seow, Heng Fong
GSK-3β phosphorylation and alteration of β-catenin in hepatocellular carcinoma
author_facet Ban, Ke Chen
Singh, Harjit
Krishnan, R.
Seow, Heng Fong
author_sort Ban, Ke Chen
title GSK-3β phosphorylation and alteration of β-catenin in hepatocellular carcinoma
title_short GSK-3β phosphorylation and alteration of β-catenin in hepatocellular carcinoma
title_full GSK-3β phosphorylation and alteration of β-catenin in hepatocellular carcinoma
title_fullStr GSK-3β phosphorylation and alteration of β-catenin in hepatocellular carcinoma
title_full_unstemmed GSK-3β phosphorylation and alteration of β-catenin in hepatocellular carcinoma
title_sort gsk-3β phosphorylation and alteration of β-catenin in hepatocellular carcinoma
publisher Elsevier Ireland
publishDate 2003
url http://psasir.upm.edu.my/id/eprint/114566/1/114566.pdf
http://psasir.upm.edu.my/id/eprint/114566/
https://linkinghub.elsevier.com/retrieve/pii/S030438350300421X
_version_ 1825810715003322368
score 13.244413

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