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Isolation and screening of an extracellular organic solvent-tolerant protease producer

An extracellular organic solvent-tolerant protease producer has been successfully isolated out of 11 isolates of benzene-toluene-xylene-ethylbenzene (BTEX) tolerant bacteria. This organic solvent-tolerant microorganism was found to be a polycyclic-aromatic-hydrocarbons (PAHs) degrader and identified...

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Main Authors: Geok, Lee Poh, Nyonya, Che, Razak, Abdul, Abd Rahman, Raja Noor Zaliha
Format: Article
Language:English
Published: Elsevier Science B.V. 2003
Online Access:http://psasir.upm.edu.my/id/eprint/114458/1/114458.pdf
http://psasir.upm.edu.my/id/eprint/114458/
https://linkinghub.elsevier.com/retrieve/pii/S1369703X02001377
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spelling my.upm.eprints.1144582025-02-05T03:55:37Z http://psasir.upm.edu.my/id/eprint/114458/ Isolation and screening of an extracellular organic solvent-tolerant protease producer Geok, Lee Poh Nyonya, Che Razak, Abdul Abd Rahman, Raja Noor Zaliha An extracellular organic solvent-tolerant protease producer has been successfully isolated out of 11 isolates of benzene-toluene-xylene-ethylbenzene (BTEX) tolerant bacteria. This organic solvent-tolerant microorganism was found to be a polycyclic-aromatic-hydrocarbons (PAHs) degrader and identified as Pseudomonas aeruginosa strain K. It was selected based on the stability of its proteolytic enzyme in the presence of various organic solvents. P. aeruginosa strain K protease tolerated up to at least 50% (v/v) of benzene, n-hexane, 1-decanol, isooctane and n-hexadecane and was also stable in the presence of 25% (v/v) n-decane and n-dodecane. This enzyme strain K was activated 2.5, 1.5 and 1.2 times by 75% (v/v) of 1-decanol, isooctane and n-dodecane, respectively. This organic solvent stable protease could be used as a biocatalyst for enzymatic synthesis in the presence of organic solvents. Elsevier Science B.V. 2003 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/114458/1/114458.pdf Geok, Lee Poh and Nyonya, Che and Razak, Abdul and Abd Rahman, Raja Noor Zaliha (2003) Isolation and screening of an extracellular organic solvent-tolerant protease producer. Biochemical Engineering Journal, 13 (1). pp. 73-77. ISSN 1369-703X; eISSN: 1369-703X https://linkinghub.elsevier.com/retrieve/pii/S1369703X02001377 10.1016/S1369-703X(02)00137-7
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description An extracellular organic solvent-tolerant protease producer has been successfully isolated out of 11 isolates of benzene-toluene-xylene-ethylbenzene (BTEX) tolerant bacteria. This organic solvent-tolerant microorganism was found to be a polycyclic-aromatic-hydrocarbons (PAHs) degrader and identified as Pseudomonas aeruginosa strain K. It was selected based on the stability of its proteolytic enzyme in the presence of various organic solvents. P. aeruginosa strain K protease tolerated up to at least 50% (v/v) of benzene, n-hexane, 1-decanol, isooctane and n-hexadecane and was also stable in the presence of 25% (v/v) n-decane and n-dodecane. This enzyme strain K was activated 2.5, 1.5 and 1.2 times by 75% (v/v) of 1-decanol, isooctane and n-dodecane, respectively. This organic solvent stable protease could be used as a biocatalyst for enzymatic synthesis in the presence of organic solvents.
format Article
author Geok, Lee Poh
Nyonya, Che
Razak, Abdul
Abd Rahman, Raja Noor Zaliha
spellingShingle Geok, Lee Poh
Nyonya, Che
Razak, Abdul
Abd Rahman, Raja Noor Zaliha
Isolation and screening of an extracellular organic solvent-tolerant protease producer
author_facet Geok, Lee Poh
Nyonya, Che
Razak, Abdul
Abd Rahman, Raja Noor Zaliha
author_sort Geok, Lee Poh
title Isolation and screening of an extracellular organic solvent-tolerant protease producer
title_short Isolation and screening of an extracellular organic solvent-tolerant protease producer
title_full Isolation and screening of an extracellular organic solvent-tolerant protease producer
title_fullStr Isolation and screening of an extracellular organic solvent-tolerant protease producer
title_full_unstemmed Isolation and screening of an extracellular organic solvent-tolerant protease producer
title_sort isolation and screening of an extracellular organic solvent-tolerant protease producer
publisher Elsevier Science B.V.
publishDate 2003
url http://psasir.upm.edu.my/id/eprint/114458/1/114458.pdf
http://psasir.upm.edu.my/id/eprint/114458/
https://linkinghub.elsevier.com/retrieve/pii/S1369703X02001377
_version_ 1823276362088054784
score 13.244413

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