Cover Image

In vitro and in silico studies of Lawsone on inflammation-induced skin cells for development of skin anti-inflammatory treatment

Lawsone, an active phytoconstituent of Lawsonia inermis sp., is proposed as a safer alternative for the current skin anti-inflammatory treatments that are mainly steroidal with unwanted side effects which does not conform to the concept of halalan toyyiban in medicine. This study aims to investiga...

Full description

Saved in:
Bibliographic Details
Main Authors: Abdullah, Shazleen Sofea, Mohammad Latif, Muhammad Alif, Md Tohid, Siti Farah
Format: Article
Language:English
Published: Malaysian Society of Applied Biology 2024
Online Access:http://psasir.upm.edu.my/id/eprint/114365/1/114365.pdf
http://psasir.upm.edu.my/id/eprint/114365/
https://jms.mabjournal.com/index.php/mab/article/view/2801
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.114365
record_format eprints
spelling my.upm.eprints.1143652025-01-20T00:50:55Z http://psasir.upm.edu.my/id/eprint/114365/ In vitro and in silico studies of Lawsone on inflammation-induced skin cells for development of skin anti-inflammatory treatment Abdullah, Shazleen Sofea Mohammad Latif, Muhammad Alif Md Tohid, Siti Farah Lawsone, an active phytoconstituent of Lawsonia inermis sp., is proposed as a safer alternative for the current skin anti-inflammatory treatments that are mainly steroidal with unwanted side effects which does not conform to the concept of halalan toyyiban in medicine. This study aims to investigate the inhibitory mechanism of lawsone on pro-inflammatory cytokine, TNF (tumour necrosis factor)-α through in silico and in vitro methods. The inhibitory mechanism of lawsone were investigated via molecular docking and molecular dynamics which were performed via AutoDock Vina and GROMACS softwares, respectively. The results were then analysed via PyMol, Proteins Plus, RMSD and RMSF graphs. Subsequently, cytotoxicity of lawsone towards A431 skin epidermoid and 3T3 fibroblast cells, was determined via MTT assay. Lawsone was further tested on A431 cells stimulated with ethanol (200 mM) or hydrogen peroxide (250 µM) for 24 hours (acute) and 48 hours (chronic) to induce pro-inflammatory cytokine (TNF-α) release. Cell viabilities were determined via MTT assay and expression of TNF-α were measured via ELISA. In silico works predicted lawsone’s ability to bind to TNF-α with good binding affinity without disruption to the protein’s structure stability.Lawsone is proven to be safe towards non-pathological cells. Lawsone exhibited significant anti-inflammatory effect on inflammation induced A431 epidermoid cells. ELISA results were not as expected as compared to previous in silico study, but an anti-inflammatory pattern can be observed in the chronic ethanol-induced treatment groups. In conclusion, lawsone is shown to have potential to be developed as a halalan- toyyiban alternative for skin anti-inflammatory treatment. Malaysian Society of Applied Biology 2024-09 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/114365/1/114365.pdf Abdullah, Shazleen Sofea and Mohammad Latif, Muhammad Alif and Md Tohid, Siti Farah (2024) In vitro and in silico studies of Lawsone on inflammation-induced skin cells for development of skin anti-inflammatory treatment. Malaysian Applied Biology, 53 (3). pp. 197-209. ISSN 0126-8643; eISSN: 2462-151X https://jms.mabjournal.com/index.php/mab/article/view/2801 10.55230/mabjournal.v53i3.2801
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Lawsone, an active phytoconstituent of Lawsonia inermis sp., is proposed as a safer alternative for the current skin anti-inflammatory treatments that are mainly steroidal with unwanted side effects which does not conform to the concept of halalan toyyiban in medicine. This study aims to investigate the inhibitory mechanism of lawsone on pro-inflammatory cytokine, TNF (tumour necrosis factor)-α through in silico and in vitro methods. The inhibitory mechanism of lawsone were investigated via molecular docking and molecular dynamics which were performed via AutoDock Vina and GROMACS softwares, respectively. The results were then analysed via PyMol, Proteins Plus, RMSD and RMSF graphs. Subsequently, cytotoxicity of lawsone towards A431 skin epidermoid and 3T3 fibroblast cells, was determined via MTT assay. Lawsone was further tested on A431 cells stimulated with ethanol (200 mM) or hydrogen peroxide (250 µM) for 24 hours (acute) and 48 hours (chronic) to induce pro-inflammatory cytokine (TNF-α) release. Cell viabilities were determined via MTT assay and expression of TNF-α were measured via ELISA. In silico works predicted lawsone’s ability to bind to TNF-α with good binding affinity without disruption to the protein’s structure stability.Lawsone is proven to be safe towards non-pathological cells. Lawsone exhibited significant anti-inflammatory effect on inflammation induced A431 epidermoid cells. ELISA results were not as expected as compared to previous in silico study, but an anti-inflammatory pattern can be observed in the chronic ethanol-induced treatment groups. In conclusion, lawsone is shown to have potential to be developed as a halalan- toyyiban alternative for skin anti-inflammatory treatment.
format Article
author Abdullah, Shazleen Sofea
Mohammad Latif, Muhammad Alif
Md Tohid, Siti Farah
spellingShingle Abdullah, Shazleen Sofea
Mohammad Latif, Muhammad Alif
Md Tohid, Siti Farah
In vitro and in silico studies of Lawsone on inflammation-induced skin cells for development of skin anti-inflammatory treatment
author_facet Abdullah, Shazleen Sofea
Mohammad Latif, Muhammad Alif
Md Tohid, Siti Farah
author_sort Abdullah, Shazleen Sofea
title In vitro and in silico studies of Lawsone on inflammation-induced skin cells for development of skin anti-inflammatory treatment
title_short In vitro and in silico studies of Lawsone on inflammation-induced skin cells for development of skin anti-inflammatory treatment
title_full In vitro and in silico studies of Lawsone on inflammation-induced skin cells for development of skin anti-inflammatory treatment
title_fullStr In vitro and in silico studies of Lawsone on inflammation-induced skin cells for development of skin anti-inflammatory treatment
title_full_unstemmed In vitro and in silico studies of Lawsone on inflammation-induced skin cells for development of skin anti-inflammatory treatment
title_sort in vitro and in silico studies of lawsone on inflammation-induced skin cells for development of skin anti-inflammatory treatment
publisher Malaysian Society of Applied Biology
publishDate 2024
url http://psasir.upm.edu.my/id/eprint/114365/1/114365.pdf
http://psasir.upm.edu.my/id/eprint/114365/
https://jms.mabjournal.com/index.php/mab/article/view/2801
_version_ 1823093132534743040
score 13.244413

Similar Items