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Fluorescent imaging and bio-cellular uptake assessment of gold-near infrared dye conjugated cockle shell calcium carbonate nanoparticles

In recent years, fluorescent imaging has emerged as an active area of interest in medical imaging. Fluorescent imaging plays a critical role in molecular imaging. Evidence suggests its use in providing a detailed structural outlook and genetic and cellular operatives of the body procedures on a mole...

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Main Authors: Kiranda, H.K., Mahmud, R.B., Mahmood, S.K., Zakaria, Z.A.
Format: Article
Language:English
Published: University of Mosul - College of Veterinary Medicine 2024
Online Access:http://psasir.upm.edu.my/id/eprint/114354/1/114354.pdf
http://psasir.upm.edu.my/id/eprint/114354/
https://www.vetmedmosul.com/article_184694.html
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spelling my.upm.eprints.1143542025-01-14T07:11:15Z http://psasir.upm.edu.my/id/eprint/114354/ Fluorescent imaging and bio-cellular uptake assessment of gold-near infrared dye conjugated cockle shell calcium carbonate nanoparticles Kiranda, H.K. Mahmud, R.B. Mahmood, S.K. Zakaria, Z.A. In recent years, fluorescent imaging has emerged as an active area of interest in medical imaging. Fluorescent imaging plays a critical role in molecular imaging. Evidence suggests its use in providing a detailed structural outlook and genetic and cellular operatives of the body procedures on a molecular plane. Imaging agents are identified to be related to risks such as not biologically disintegration and great poisonousness. Researchers have shown a keen attentiveness to the growth of targeted multifunctional agents in oncology and near-infrared (NIR) fluorescence imaging. This study assessed fluorescent imaging and bio-cellular acceptance of the gold (NIR) conjugated cockle shell-derived calcium carbonate nanoparticles Au-CsCaCO3NPs. The synthesized Au-CsCaCO3NPs were characterized by Transmission electron microscopy (TEM) for size and morphology, Zeta potential, and UV-Vis spectrophotometer. Biocompatibility of Au-CsCaCO3NPs in cultured human breast carcinoma cells MCF-7 and mouse embryonic fibroblast cells NIH3T3 was evaluated using bioassays like Lactate Dehydrogenase LDH and Reactive Oxygen Species ROS for toxic examination. Cellular morphology and uptake were studied by fluorescence and confocal microscopy. The outcomes proved that MCF-7 treated Au-CsCaCO3NP cells observed more cell deaths than NIH3T3 treated Au-CsCaCO3NP cells. Additionally, the cells were capable of assuming nanoparticles within their cellular compartments. In conclusion, gold-near infrared dye conjugated cockle shell calcium carbonate nanoparticles Au-CsCaCO3NPs were easily synthesized, biocompatible, and environmentally friendly. It is safe to state that the Au-CsCaCO3NPs could be used for imaging and could present opportunities for progressing cancer imaging. University of Mosul - College of Veterinary Medicine 2024-10-01 Article PeerReviewed text en cc_by_4 http://psasir.upm.edu.my/id/eprint/114354/1/114354.pdf Kiranda, H.K. and Mahmud, R.B. and Mahmood, S.K. and Zakaria, Z.A. (2024) Fluorescent imaging and bio-cellular uptake assessment of gold-near infrared dye conjugated cockle shell calcium carbonate nanoparticles. Iraqi Journal of Veterinary Sciences, 38 (4). pp. 721-730. ISSN 1607-3894; eISSN: 2071-1255 https://www.vetmedmosul.com/article_184694.html 10.33899/ijvs.2024.146804.3470
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description In recent years, fluorescent imaging has emerged as an active area of interest in medical imaging. Fluorescent imaging plays a critical role in molecular imaging. Evidence suggests its use in providing a detailed structural outlook and genetic and cellular operatives of the body procedures on a molecular plane. Imaging agents are identified to be related to risks such as not biologically disintegration and great poisonousness. Researchers have shown a keen attentiveness to the growth of targeted multifunctional agents in oncology and near-infrared (NIR) fluorescence imaging. This study assessed fluorescent imaging and bio-cellular acceptance of the gold (NIR) conjugated cockle shell-derived calcium carbonate nanoparticles Au-CsCaCO3NPs. The synthesized Au-CsCaCO3NPs were characterized by Transmission electron microscopy (TEM) for size and morphology, Zeta potential, and UV-Vis spectrophotometer. Biocompatibility of Au-CsCaCO3NPs in cultured human breast carcinoma cells MCF-7 and mouse embryonic fibroblast cells NIH3T3 was evaluated using bioassays like Lactate Dehydrogenase LDH and Reactive Oxygen Species ROS for toxic examination. Cellular morphology and uptake were studied by fluorescence and confocal microscopy. The outcomes proved that MCF-7 treated Au-CsCaCO3NP cells observed more cell deaths than NIH3T3 treated Au-CsCaCO3NP cells. Additionally, the cells were capable of assuming nanoparticles within their cellular compartments. In conclusion, gold-near infrared dye conjugated cockle shell calcium carbonate nanoparticles Au-CsCaCO3NPs were easily synthesized, biocompatible, and environmentally friendly. It is safe to state that the Au-CsCaCO3NPs could be used for imaging and could present opportunities for progressing cancer imaging.
format Article
author Kiranda, H.K.
Mahmud, R.B.
Mahmood, S.K.
Zakaria, Z.A.
spellingShingle Kiranda, H.K.
Mahmud, R.B.
Mahmood, S.K.
Zakaria, Z.A.
Fluorescent imaging and bio-cellular uptake assessment of gold-near infrared dye conjugated cockle shell calcium carbonate nanoparticles
author_facet Kiranda, H.K.
Mahmud, R.B.
Mahmood, S.K.
Zakaria, Z.A.
author_sort Kiranda, H.K.
title Fluorescent imaging and bio-cellular uptake assessment of gold-near infrared dye conjugated cockle shell calcium carbonate nanoparticles
title_short Fluorescent imaging and bio-cellular uptake assessment of gold-near infrared dye conjugated cockle shell calcium carbonate nanoparticles
title_full Fluorescent imaging and bio-cellular uptake assessment of gold-near infrared dye conjugated cockle shell calcium carbonate nanoparticles
title_fullStr Fluorescent imaging and bio-cellular uptake assessment of gold-near infrared dye conjugated cockle shell calcium carbonate nanoparticles
title_full_unstemmed Fluorescent imaging and bio-cellular uptake assessment of gold-near infrared dye conjugated cockle shell calcium carbonate nanoparticles
title_sort fluorescent imaging and bio-cellular uptake assessment of gold-near infrared dye conjugated cockle shell calcium carbonate nanoparticles
publisher University of Mosul - College of Veterinary Medicine
publishDate 2024
url http://psasir.upm.edu.my/id/eprint/114354/1/114354.pdf
http://psasir.upm.edu.my/id/eprint/114354/
https://www.vetmedmosul.com/article_184694.html
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score 13.244413

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