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A newly isolated organic solvent tolerant Bacillus sphaericus 205y producing organic solvent-stable lipase

A total of 131 organic solvent tolerant isolates originating from soil samples were successfully isolated via direct plating method using 1% (v/v) of either benzene, toluene, or mixture of benzene and toluene as their sole carbon source. Six isolates demonstrated high tolerance of up to 75% (v/v) co...

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Main Authors: Hun, Chin John, Raja Abd Rahman, Raja Noor Zaliha, Salleh, Abu Bakar, Basri, Mahiran
Format: Article
Language:English
Published: Elsevier Science B.V. 2003
Online Access:http://psasir.upm.edu.my/id/eprint/114350/1/114350.pdf
http://psasir.upm.edu.my/id/eprint/114350/
https://linkinghub.elsevier.com/retrieve/pii/S1369703X02001857
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spelling my.upm.eprints.1143502025-02-05T03:34:17Z http://psasir.upm.edu.my/id/eprint/114350/ A newly isolated organic solvent tolerant Bacillus sphaericus 205y producing organic solvent-stable lipase Hun, Chin John Raja Abd Rahman, Raja Noor Zaliha Salleh, Abu Bakar Basri, Mahiran A total of 131 organic solvent tolerant isolates originating from soil samples were successfully isolated via direct plating method using 1% (v/v) of either benzene, toluene, or mixture of benzene and toluene as their sole carbon source. Six isolates demonstrated high tolerance of up to 75% (v/v) concentration of BTEX (n-benzene, toluene, ethyl benzene and p-xylene). These isolates were screened for lipolytic activity using triolein plates. Isolate 205y was found to produce the highest lipase yield in liquid medium and later identified as Bacillus sphaericus by biochemical test and 16S ribosomal DNA sequence. No work on B. sphaericus producing lipase has been reported so far. Four production media were tested and medium M3 yielded the highest lipase activity of 0.42 U ml-1 min-1 at 36 h incubation time. Stability studies in various organic solvents at 25% (v/v) showed that lipase from B. sphaericus 205y were not only stable but also activated by n-hexane and p-xylene by 3.5- and 2.9-folds, respectively. The activity, however, was greatly reduced in dimethyl sulfoxide and was completely inactivated by hexadecane and acetonitrile. Elsevier Science B.V. 2003 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/114350/1/114350.pdf Hun, Chin John and Raja Abd Rahman, Raja Noor Zaliha and Salleh, Abu Bakar and Basri, Mahiran (2003) A newly isolated organic solvent tolerant Bacillus sphaericus 205y producing organic solvent-stable lipase. Biochemical Engineering Journal, 15 (2). pp. 147-151. ISSN 1369-703X; eISSN: 1369-703X https://linkinghub.elsevier.com/retrieve/pii/S1369703X02001857 10.1016/S1369-703X(02)00185-7
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description A total of 131 organic solvent tolerant isolates originating from soil samples were successfully isolated via direct plating method using 1% (v/v) of either benzene, toluene, or mixture of benzene and toluene as their sole carbon source. Six isolates demonstrated high tolerance of up to 75% (v/v) concentration of BTEX (n-benzene, toluene, ethyl benzene and p-xylene). These isolates were screened for lipolytic activity using triolein plates. Isolate 205y was found to produce the highest lipase yield in liquid medium and later identified as Bacillus sphaericus by biochemical test and 16S ribosomal DNA sequence. No work on B. sphaericus producing lipase has been reported so far. Four production media were tested and medium M3 yielded the highest lipase activity of 0.42 U ml-1 min-1 at 36 h incubation time. Stability studies in various organic solvents at 25% (v/v) showed that lipase from B. sphaericus 205y were not only stable but also activated by n-hexane and p-xylene by 3.5- and 2.9-folds, respectively. The activity, however, was greatly reduced in dimethyl sulfoxide and was completely inactivated by hexadecane and acetonitrile.
format Article
author Hun, Chin John
Raja Abd Rahman, Raja Noor Zaliha
Salleh, Abu Bakar
Basri, Mahiran
spellingShingle Hun, Chin John
Raja Abd Rahman, Raja Noor Zaliha
Salleh, Abu Bakar
Basri, Mahiran
A newly isolated organic solvent tolerant Bacillus sphaericus 205y producing organic solvent-stable lipase
author_facet Hun, Chin John
Raja Abd Rahman, Raja Noor Zaliha
Salleh, Abu Bakar
Basri, Mahiran
author_sort Hun, Chin John
title A newly isolated organic solvent tolerant Bacillus sphaericus 205y producing organic solvent-stable lipase
title_short A newly isolated organic solvent tolerant Bacillus sphaericus 205y producing organic solvent-stable lipase
title_full A newly isolated organic solvent tolerant Bacillus sphaericus 205y producing organic solvent-stable lipase
title_fullStr A newly isolated organic solvent tolerant Bacillus sphaericus 205y producing organic solvent-stable lipase
title_full_unstemmed A newly isolated organic solvent tolerant Bacillus sphaericus 205y producing organic solvent-stable lipase
title_sort newly isolated organic solvent tolerant bacillus sphaericus 205y producing organic solvent-stable lipase
publisher Elsevier Science B.V.
publishDate 2003
url http://psasir.upm.edu.my/id/eprint/114350/1/114350.pdf
http://psasir.upm.edu.my/id/eprint/114350/
https://linkinghub.elsevier.com/retrieve/pii/S1369703X02001857
_version_ 1823276356562059264
score 13.244413

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