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Polyphenoloxidase from guava (Psidium guajava L.)

Polyphenoloxidase (PPO) was isolated from guava (Psidium guajava L., cv. Kampuchea). Purification by acetone precipitation and dialysis of the Amicon concentrate resulted in 4.9‐fold and 17.2‐fold purification of the dialysed crude extract respectively. Polyacrylamide gel electrophoresis indicated t...

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Main Authors: Augustin, M.A., Ghazali, Hasanah M., Hashim, Hasimah
Format: Article
Published: John Wiley & Sons 1985
Online Access:http://psasir.upm.edu.my/id/eprint/114332/
https://onlinelibrary.wiley.com/doi/10.1002/jsfa.2740361209
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spelling my.upm.eprints.1143322025-01-14T02:57:23Z http://psasir.upm.edu.my/id/eprint/114332/ Polyphenoloxidase from guava (Psidium guajava L.) Augustin, M.A. Ghazali, Hasanah M. Hashim, Hasimah Polyphenoloxidase (PPO) was isolated from guava (Psidium guajava L., cv. Kampuchea). Purification by acetone precipitation and dialysis of the Amicon concentrate resulted in 4.9‐fold and 17.2‐fold purification of the dialysed crude extract respectively. Polyacrylamide gel electrophoresis indicated that PPO had a molecular weight in the region of 120000–140000. The pH optimum of the enzyme was found to be 7.2. Heat inactivation studies showed that heating for 1.1, 3.5, and 10.1 min at 75, 65 and 55°C respectively caused a 50% loss in enzymic activity. The enzyme‐catalysed browning reaction was significantly inhibited in the presence of sodium metabisulphite, L‐cysteine hydrochloride and ascorbic acid. John Wiley & Sons 1985 Article PeerReviewed Augustin, M.A. and Ghazali, Hasanah M. and Hashim, Hasimah (1985) Polyphenoloxidase from guava (Psidium guajava L.). Journal of the Science of Food and Agriculture, 36 (12). pp. 1259-1265. ISSN 0022-5142; eISSN: 1097-0010 https://onlinelibrary.wiley.com/doi/10.1002/jsfa.2740361209 10.1002/jsfa.2740361209
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description Polyphenoloxidase (PPO) was isolated from guava (Psidium guajava L., cv. Kampuchea). Purification by acetone precipitation and dialysis of the Amicon concentrate resulted in 4.9‐fold and 17.2‐fold purification of the dialysed crude extract respectively. Polyacrylamide gel electrophoresis indicated that PPO had a molecular weight in the region of 120000–140000. The pH optimum of the enzyme was found to be 7.2. Heat inactivation studies showed that heating for 1.1, 3.5, and 10.1 min at 75, 65 and 55°C respectively caused a 50% loss in enzymic activity. The enzyme‐catalysed browning reaction was significantly inhibited in the presence of sodium metabisulphite, L‐cysteine hydrochloride and ascorbic acid.
format Article
author Augustin, M.A.
Ghazali, Hasanah M.
Hashim, Hasimah
spellingShingle Augustin, M.A.
Ghazali, Hasanah M.
Hashim, Hasimah
Polyphenoloxidase from guava (Psidium guajava L.)
author_facet Augustin, M.A.
Ghazali, Hasanah M.
Hashim, Hasimah
author_sort Augustin, M.A.
title Polyphenoloxidase from guava (Psidium guajava L.)
title_short Polyphenoloxidase from guava (Psidium guajava L.)
title_full Polyphenoloxidase from guava (Psidium guajava L.)
title_fullStr Polyphenoloxidase from guava (Psidium guajava L.)
title_full_unstemmed Polyphenoloxidase from guava (Psidium guajava L.)
title_sort polyphenoloxidase from guava (psidium guajava l.)
publisher John Wiley & Sons
publishDate 1985
url http://psasir.upm.edu.my/id/eprint/114332/
https://onlinelibrary.wiley.com/doi/10.1002/jsfa.2740361209
_version_ 1823093124896915456
score 13.23648

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