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Isolation and characterisation of Pink Pigmented Facultative Methylotrophs (PPFM) from Malaysian ulam leaves

This study aims to isolate and characterise the Pink Pigmented Facultative Methylotrophs (PPFM) bacteria from the leaves of common Malaysian table salad (ulam). The colonies of PPFM bacteria were obtained using selectively modified Pseudomonas agar based on the appearance of the pink pigment colony....

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Main Authors: Ramli, Nur Isti'anah, Abas, Faridah, Ismail, Intan Safinar, Rukayadi, Yaya, Jurime, Nuramiratul Hafizah
Format: Article
Language:English
Published: Journal of Pure and Applied Microbiology 2024
Online Access:http://psasir.upm.edu.my/id/eprint/114227/1/114227.pdf
http://psasir.upm.edu.my/id/eprint/114227/
https://microbiologyjournal.org/isolation-and-characterisation-of-pink-pigmented-facultative-methylotrophs-ppfm-from-malaysian-ulam-leaves/
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spelling my.upm.eprints.1142272025-01-10T07:46:00Z http://psasir.upm.edu.my/id/eprint/114227/ Isolation and characterisation of Pink Pigmented Facultative Methylotrophs (PPFM) from Malaysian ulam leaves Ramli, Nur Isti'anah Abas, Faridah Ismail, Intan Safinar Rukayadi, Yaya Jurime, Nuramiratul Hafizah This study aims to isolate and characterise the Pink Pigmented Facultative Methylotrophs (PPFM) bacteria from the leaves of common Malaysian table salad (ulam). The colonies of PPFM bacteria were obtained using selectively modified Pseudomonas agar based on the appearance of the pink pigment colony. The three selected isolates labelled OJ4, OJ154 [Oenanthe javanica (Blume) DC.] and ML8 [from Melicope lunu-ankenda (Gaertn.) T. G. Hartley] were chosen for characterisation. The result showed the PPFM bacteria colonies (log CFU/g) at the leaf surface of C. caudatus are 4.4 ± 0.1, significantly higher than O. javanica, 3.8 ± 0.2, and M. lunu-ankenda, 3.2 ± 0.1. The selected isolates belong to the Gram-negative group, motile with rod shape with size [length (l) × width (w)] in µm unit 4.3 ± 1.1 × 1.6 ± 0.6, 5.4 ± 0.2 × 1.2 ± 0.1, and 3.5 ± 0.7 × 1.0 ± 0.0, respectively. They show positive urease, catalase, and oxidase activities, while none of them can degrade starch, gelatine, or cellulose, as well as glucose fermentation (MR test) and metabolism actions (VP test), producing indole and hydrogen sulphide gaseous. Only isolate OJ154 demonstrates positive casein hydrolysis and nitrate reduction activities, while only isolate ML8 can utilise citrate but not in lipid degradation. Their sequence analysis of the 16S rRNA indicated that OJ4 and ML8 are Methylobacterium radiotolerans with a similarity of 99%, whereas, OJ154 is Methylorubrum salsuginis with a similarity of 99%. To conclude, PPFM bacteria from the leaves of C. caudatus, O. javanica, and M. lunu-ankenda have been isolated and characterised in particular. © The Author(s) 2024. Open Access. Journal of Pure and Applied Microbiology 2024-08-29 Article PeerReviewed text en cc_by_4 http://psasir.upm.edu.my/id/eprint/114227/1/114227.pdf Ramli, Nur Isti'anah and Abas, Faridah and Ismail, Intan Safinar and Rukayadi, Yaya and Jurime, Nuramiratul Hafizah (2024) Isolation and characterisation of Pink Pigmented Facultative Methylotrophs (PPFM) from Malaysian ulam leaves. Journal of Pure and Applied Microbiology, 18 (3). pp. 1903-1919. ISSN 0973-7510; eISSN: 2581-690X https://microbiologyjournal.org/isolation-and-characterisation-of-pink-pigmented-facultative-methylotrophs-ppfm-from-malaysian-ulam-leaves/ 10.22207/JPAM.18.3.41
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description This study aims to isolate and characterise the Pink Pigmented Facultative Methylotrophs (PPFM) bacteria from the leaves of common Malaysian table salad (ulam). The colonies of PPFM bacteria were obtained using selectively modified Pseudomonas agar based on the appearance of the pink pigment colony. The three selected isolates labelled OJ4, OJ154 [Oenanthe javanica (Blume) DC.] and ML8 [from Melicope lunu-ankenda (Gaertn.) T. G. Hartley] were chosen for characterisation. The result showed the PPFM bacteria colonies (log CFU/g) at the leaf surface of C. caudatus are 4.4 ± 0.1, significantly higher than O. javanica, 3.8 ± 0.2, and M. lunu-ankenda, 3.2 ± 0.1. The selected isolates belong to the Gram-negative group, motile with rod shape with size [length (l) × width (w)] in µm unit 4.3 ± 1.1 × 1.6 ± 0.6, 5.4 ± 0.2 × 1.2 ± 0.1, and 3.5 ± 0.7 × 1.0 ± 0.0, respectively. They show positive urease, catalase, and oxidase activities, while none of them can degrade starch, gelatine, or cellulose, as well as glucose fermentation (MR test) and metabolism actions (VP test), producing indole and hydrogen sulphide gaseous. Only isolate OJ154 demonstrates positive casein hydrolysis and nitrate reduction activities, while only isolate ML8 can utilise citrate but not in lipid degradation. Their sequence analysis of the 16S rRNA indicated that OJ4 and ML8 are Methylobacterium radiotolerans with a similarity of 99%, whereas, OJ154 is Methylorubrum salsuginis with a similarity of 99%. To conclude, PPFM bacteria from the leaves of C. caudatus, O. javanica, and M. lunu-ankenda have been isolated and characterised in particular. © The Author(s) 2024. Open Access.
format Article
author Ramli, Nur Isti'anah
Abas, Faridah
Ismail, Intan Safinar
Rukayadi, Yaya
Jurime, Nuramiratul Hafizah
spellingShingle Ramli, Nur Isti'anah
Abas, Faridah
Ismail, Intan Safinar
Rukayadi, Yaya
Jurime, Nuramiratul Hafizah
Isolation and characterisation of Pink Pigmented Facultative Methylotrophs (PPFM) from Malaysian ulam leaves
author_facet Ramli, Nur Isti'anah
Abas, Faridah
Ismail, Intan Safinar
Rukayadi, Yaya
Jurime, Nuramiratul Hafizah
author_sort Ramli, Nur Isti'anah
title Isolation and characterisation of Pink Pigmented Facultative Methylotrophs (PPFM) from Malaysian ulam leaves
title_short Isolation and characterisation of Pink Pigmented Facultative Methylotrophs (PPFM) from Malaysian ulam leaves
title_full Isolation and characterisation of Pink Pigmented Facultative Methylotrophs (PPFM) from Malaysian ulam leaves
title_fullStr Isolation and characterisation of Pink Pigmented Facultative Methylotrophs (PPFM) from Malaysian ulam leaves
title_full_unstemmed Isolation and characterisation of Pink Pigmented Facultative Methylotrophs (PPFM) from Malaysian ulam leaves
title_sort isolation and characterisation of pink pigmented facultative methylotrophs (ppfm) from malaysian ulam leaves
publisher Journal of Pure and Applied Microbiology
publishDate 2024
url http://psasir.upm.edu.my/id/eprint/114227/1/114227.pdf
http://psasir.upm.edu.my/id/eprint/114227/
https://microbiologyjournal.org/isolation-and-characterisation-of-pink-pigmented-facultative-methylotrophs-ppfm-from-malaysian-ulam-leaves/
_version_ 1821003747665379328
score 13.244413

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