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Synthesis of palm kernel oil alkanolamide using lipase

Fatty monoethanolamides were synthesized in organic solvent from palm kernel olein (PKL) and palm kernel stearin (PKS) using a lipase from Candida rugosa. The transamidation reactions of PKL and PKS were enhanced in the presence of lipase. The optimal yield was achieved at reaction time 72 hours for...

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Main Authors: Abdul Rahman, M.B., Yap, C.L., Dzulkefly, K., Abdul Rahman, R.N.Z.
Format: Article
Language:English
Published: 2003
Online Access:http://psasir.upm.edu.my/id/eprint/114113/1/114113.pdf
http://psasir.upm.edu.my/id/eprint/114113/
https://www.jstage.jst.go.jp/article/jos/52/2/52_2_65/_article
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spelling my.upm.eprints.1141132024-12-10T01:48:13Z http://psasir.upm.edu.my/id/eprint/114113/ Synthesis of palm kernel oil alkanolamide using lipase Abdul Rahman, M.B. Yap, C.L. Dzulkefly, K. Abdul Rahman, R.N.Z. Fatty monoethanolamides were synthesized in organic solvent from palm kernel olein (PKL) and palm kernel stearin (PKS) using a lipase from Candida rugosa. The transamidation reactions of PKL and PKS were enhanced in the presence of lipase. The optimal yield was achieved at reaction time 72 hours for both systems with PKL and PKS as the substrates. For PKL as the substrates, the optimal reaction temperature was 40°C, whereas with PKS as the substrate, no optimum temperature was found (in the range of temperature studied) where the relative yield increased with the increasing temperature. Lipase functioned better in hydrophobic solvents compared to hydrophilic solvents. The best solvent for the reactions was isooctane. Increasing the amount of the monoethanolamine used resulted in the increasing solubility of the reactants and products, hence, increasing the yield of the product. For PKL, increasing the mole ratio of PKL:monoethanolamine from 1:1 to 1:15 increased the relative yield to 4.5 fold. However, for PKS, the increase was only 2.5 fold. The optimal ratio of enzyme/PKL (or PKS) was 0.035. In the water activity studies, the preequilibrium and the direct salt hydrate addition methods were used. Generally, PKL always showed the higher relative yield compared to PKS. At the optimum condition at room temperature, the yield of PKL monoethanolamide was 77.0% and the yield of PKS monoethanolamide was 39.0%. Kinetic studies also showed a clear preference to PKL in which its Km value was 10-fold lower than that of PKS at room temperature. © 2003, Japan Oil Chemists' Society. All rights reserved. 2003 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/114113/1/114113.pdf Abdul Rahman, M.B. and Yap, C.L. and Dzulkefly, K. and Abdul Rahman, R.N.Z. (2003) Synthesis of palm kernel oil alkanolamide using lipase. Journal of Oleo Science, 52 (2). pp. 65-72. ISSN 1345-8957; eISSN: 1347-3352 https://www.jstage.jst.go.jp/article/jos/52/2/52_2_65/_article 10.5650/jos.52.65
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Fatty monoethanolamides were synthesized in organic solvent from palm kernel olein (PKL) and palm kernel stearin (PKS) using a lipase from Candida rugosa. The transamidation reactions of PKL and PKS were enhanced in the presence of lipase. The optimal yield was achieved at reaction time 72 hours for both systems with PKL and PKS as the substrates. For PKL as the substrates, the optimal reaction temperature was 40°C, whereas with PKS as the substrate, no optimum temperature was found (in the range of temperature studied) where the relative yield increased with the increasing temperature. Lipase functioned better in hydrophobic solvents compared to hydrophilic solvents. The best solvent for the reactions was isooctane. Increasing the amount of the monoethanolamine used resulted in the increasing solubility of the reactants and products, hence, increasing the yield of the product. For PKL, increasing the mole ratio of PKL:monoethanolamine from 1:1 to 1:15 increased the relative yield to 4.5 fold. However, for PKS, the increase was only 2.5 fold. The optimal ratio of enzyme/PKL (or PKS) was 0.035. In the water activity studies, the preequilibrium and the direct salt hydrate addition methods were used. Generally, PKL always showed the higher relative yield compared to PKS. At the optimum condition at room temperature, the yield of PKL monoethanolamide was 77.0% and the yield of PKS monoethanolamide was 39.0%. Kinetic studies also showed a clear preference to PKL in which its Km value was 10-fold lower than that of PKS at room temperature. © 2003, Japan Oil Chemists' Society. All rights reserved.
format Article
author Abdul Rahman, M.B.
Yap, C.L.
Dzulkefly, K.
Abdul Rahman, R.N.Z.
spellingShingle Abdul Rahman, M.B.
Yap, C.L.
Dzulkefly, K.
Abdul Rahman, R.N.Z.
Synthesis of palm kernel oil alkanolamide using lipase
author_facet Abdul Rahman, M.B.
Yap, C.L.
Dzulkefly, K.
Abdul Rahman, R.N.Z.
author_sort Abdul Rahman, M.B.
title Synthesis of palm kernel oil alkanolamide using lipase
title_short Synthesis of palm kernel oil alkanolamide using lipase
title_full Synthesis of palm kernel oil alkanolamide using lipase
title_fullStr Synthesis of palm kernel oil alkanolamide using lipase
title_full_unstemmed Synthesis of palm kernel oil alkanolamide using lipase
title_sort synthesis of palm kernel oil alkanolamide using lipase
publishDate 2003
url http://psasir.upm.edu.my/id/eprint/114113/1/114113.pdf
http://psasir.upm.edu.my/id/eprint/114113/
https://www.jstage.jst.go.jp/article/jos/52/2/52_2_65/_article
_version_ 1818835919127445504
score 13.239859

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