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Identification of RAPD markers closely linked to the mlo-locus in barley

Developing resistance to powdery mildew, Erysiphe graminis f.sp. hordei, is a major goal of many barley breeding programmes. Several resistance genes have been tagged or mapped with molecular markers. The mlo gene confers durable resistance towards all known isolates of the pathogen. In this study,...

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Main Authors: Manninen, O.M., Turpeinen, T., Nissilä, E.
Format: Article
Language:English
Published: Blackwell Publishing Ltdcustomerservices@oxonblackwellpublishing.com 1997
Online Access:http://psasir.upm.edu.my/id/eprint/114111/1/114111.pdf
http://psasir.upm.edu.my/id/eprint/114111/
https://onlinelibrary.wiley.com/doi/10.1111/j.1439-0523.1997.tb01031.x
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spelling my.upm.eprints.1141112024-12-10T01:47:57Z http://psasir.upm.edu.my/id/eprint/114111/ Identification of RAPD markers closely linked to the mlo-locus in barley Manninen, O.M. Turpeinen, T. Nissilä, E. Developing resistance to powdery mildew, Erysiphe graminis f.sp. hordei, is a major goal of many barley breeding programmes. Several resistance genes have been tagged or mapped with molecular markers. The mlo gene confers durable resistance towards all known isolates of the pathogen. In this study, RAPD markers and bulked segregant analysis were used to determine PCR-based markers linked to the mlo-locus. Sixty doubled haploid lines from a cross between an isogenic line of 'Ingrid' carrying the mlo11 allele and a susceptible cv. 'Pokko' were used as plant material. Seven linked RAPD markers were found, the closest lying 1.6 cM away from the resistance gene. When eight barley varieties were assayed for the presence of this band, F4-980, it was found in the resistant varieties but not in the susceptible ones. The linked marker bands could be amplified from DNA-samples prepared by using three different methods, including a quick squash technique. PCR-based markers linked to the resistance gene can be used as tools for selection in breeding programmes. Blackwell Publishing Ltdcustomerservices@oxonblackwellpublishing.com 1997 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/114111/1/114111.pdf Manninen, O.M. and Turpeinen, T. and Nissilä, E. (1997) Identification of RAPD markers closely linked to the mlo-locus in barley. Plant Breeding, 116 (5). pp. 461-464. ISSN 0179-9541; eISSN: 0179-9541 https://onlinelibrary.wiley.com/doi/10.1111/j.1439-0523.1997.tb01031.x 10.1111/j.1439-0523.1997.tb01031.x
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Developing resistance to powdery mildew, Erysiphe graminis f.sp. hordei, is a major goal of many barley breeding programmes. Several resistance genes have been tagged or mapped with molecular markers. The mlo gene confers durable resistance towards all known isolates of the pathogen. In this study, RAPD markers and bulked segregant analysis were used to determine PCR-based markers linked to the mlo-locus. Sixty doubled haploid lines from a cross between an isogenic line of 'Ingrid' carrying the mlo11 allele and a susceptible cv. 'Pokko' were used as plant material. Seven linked RAPD markers were found, the closest lying 1.6 cM away from the resistance gene. When eight barley varieties were assayed for the presence of this band, F4-980, it was found in the resistant varieties but not in the susceptible ones. The linked marker bands could be amplified from DNA-samples prepared by using three different methods, including a quick squash technique. PCR-based markers linked to the resistance gene can be used as tools for selection in breeding programmes.
format Article
author Manninen, O.M.
Turpeinen, T.
Nissilä, E.
spellingShingle Manninen, O.M.
Turpeinen, T.
Nissilä, E.
Identification of RAPD markers closely linked to the mlo-locus in barley
author_facet Manninen, O.M.
Turpeinen, T.
Nissilä, E.
author_sort Manninen, O.M.
title Identification of RAPD markers closely linked to the mlo-locus in barley
title_short Identification of RAPD markers closely linked to the mlo-locus in barley
title_full Identification of RAPD markers closely linked to the mlo-locus in barley
title_fullStr Identification of RAPD markers closely linked to the mlo-locus in barley
title_full_unstemmed Identification of RAPD markers closely linked to the mlo-locus in barley
title_sort identification of rapd markers closely linked to the mlo-locus in barley
publisher Blackwell Publishing Ltdcustomerservices@oxonblackwellpublishing.com
publishDate 1997
url http://psasir.upm.edu.my/id/eprint/114111/1/114111.pdf
http://psasir.upm.edu.my/id/eprint/114111/
https://onlinelibrary.wiley.com/doi/10.1111/j.1439-0523.1997.tb01031.x
_version_ 1818835918992179200
score 13.223943

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