Staff View: Molecular typing of Salmonella weltevreden and Salmonella chincol by pulsed-field gel electrophoresis (PFGE) and enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR)

Molecular typing of Salmonella weltevreden and Salmonella chincol by pulsed-field gel electrophoresis (PFGE) and enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR)

Genomic DNA of Salmonella weltevreden (10 isolates from poultry, two isolates each from raw vegetables and river water) and S. chincol (15 isolates from poultry) were characterized by pulsed-field gel electrophoresis (PFGE) and enterobacterial repetitive intergenic consensus-polymerase chain reactio...

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Main Authors:	Sahilah, A.M., Son, R., Rusul, G., Samuel, L., Hassan, Z., Lum, K.Y., Ahmad, M.A.
Format:	Article
Language:	English
Published:	Kluwer Academic Publishers 2000
Online Access:	http://psasir.upm.edu.my/id/eprint/114057/1/114057.pdf http://psasir.upm.edu.my/id/eprint/114057/ https://link.springer.com/article/10.1023/A:1008967914499?error=cookies_not_supported&code=9fbe88f4-98c7-4c34-97e5-a392ed99ff25
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id	my.upm.eprints.114057
record_format	eprints
spelling	my.upm.eprints.1140572024-12-08T08:16:15Z http://psasir.upm.edu.my/id/eprint/114057/ Molecular typing of Salmonella weltevreden and Salmonella chincol by pulsed-field gel electrophoresis (PFGE) and enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) Sahilah, A.M. Son, R. Rusul, G. Samuel, L. Hassan, Z. Lum, K.Y. Ahmad, M.A. Genomic DNA of Salmonella weltevreden (10 isolates from poultry, two isolates each from raw vegetables and river water) and S. chincol (15 isolates from poultry) were characterized by pulsed-field gel electrophoresis (PFGE) and enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) analysis. These isolates originated from a single location in Kajang, Selangor. The results of the PFGE and ERIC-PCR were analysed and comparisons were made using GelCompar software. ERIC-PCR with primers ERIC1R and ERIC2 discriminated the S. weltevreden into five clusters and two single isolates and S. chincol into two clusters and two single isolates at a similarity level of 80%, respectively. PFGE produced a single cluster and eight single isolates for S. weltevreden, and one cluster and 11 single isolates for S. chincol at a similarity level of 80% after digestion with the restriction enzyme XbaI, respectively. These results demonstrate that both PFGE and ERIC-PCR are suitable tools for molecular typing of the isolates examined. Kluwer Academic Publishers 2000 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/114057/1/114057.pdf Sahilah, A.M. and Son, R. and Rusul, G. and Samuel, L. and Hassan, Z. and Lum, K.Y. and Ahmad, M.A. (2000) Molecular typing of Salmonella weltevreden and Salmonella chincol by pulsed-field gel electrophoresis (PFGE) and enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR). World Journal of Microbiology and Biotechnology, 16 (7). pp. 621-624. ISSN 0959-3993 https://link.springer.com/article/10.1023/A:1008967914499?error=cookies_not_supported&code=9fbe88f4-98c7-4c34-97e5-a392ed99ff25 10.1023/a:1008967914499
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
language	English
description	Genomic DNA of Salmonella weltevreden (10 isolates from poultry, two isolates each from raw vegetables and river water) and S. chincol (15 isolates from poultry) were characterized by pulsed-field gel electrophoresis (PFGE) and enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) analysis. These isolates originated from a single location in Kajang, Selangor. The results of the PFGE and ERIC-PCR were analysed and comparisons were made using GelCompar software. ERIC-PCR with primers ERIC1R and ERIC2 discriminated the S. weltevreden into five clusters and two single isolates and S. chincol into two clusters and two single isolates at a similarity level of 80%, respectively. PFGE produced a single cluster and eight single isolates for S. weltevreden, and one cluster and 11 single isolates for S. chincol at a similarity level of 80% after digestion with the restriction enzyme XbaI, respectively. These results demonstrate that both PFGE and ERIC-PCR are suitable tools for molecular typing of the isolates examined.
format	Article
author	Sahilah, A.M. Son, R. Rusul, G. Samuel, L. Hassan, Z. Lum, K.Y. Ahmad, M.A.
spellingShingle	Sahilah, A.M. Son, R. Rusul, G. Samuel, L. Hassan, Z. Lum, K.Y. Ahmad, M.A. Molecular typing of Salmonella weltevreden and Salmonella chincol by pulsed-field gel electrophoresis (PFGE) and enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR)
author_facet	Sahilah, A.M. Son, R. Rusul, G. Samuel, L. Hassan, Z. Lum, K.Y. Ahmad, M.A.
author_sort	Sahilah, A.M.
title	Molecular typing of Salmonella weltevreden and Salmonella chincol by pulsed-field gel electrophoresis (PFGE) and enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR)
title_short	Molecular typing of Salmonella weltevreden and Salmonella chincol by pulsed-field gel electrophoresis (PFGE) and enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR)
title_full	Molecular typing of Salmonella weltevreden and Salmonella chincol by pulsed-field gel electrophoresis (PFGE) and enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR)
title_fullStr	Molecular typing of Salmonella weltevreden and Salmonella chincol by pulsed-field gel electrophoresis (PFGE) and enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR)
title_full_unstemmed	Molecular typing of Salmonella weltevreden and Salmonella chincol by pulsed-field gel electrophoresis (PFGE) and enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR)
title_sort	molecular typing of salmonella weltevreden and salmonella chincol by pulsed-field gel electrophoresis (pfge) and enterobacterial repetitive intergenic consensus-polymerase chain reaction (eric-pcr)
publisher	Kluwer Academic Publishers
publishDate	2000
url	http://psasir.upm.edu.my/id/eprint/114057/1/114057.pdf http://psasir.upm.edu.my/id/eprint/114057/ https://link.springer.com/article/10.1023/A:1008967914499?error=cookies_not_supported&code=9fbe88f4-98c7-4c34-97e5-a392ed99ff25
_version_	1818835916750323712
score	13.223943

Molecular typing of Salmonella weltevreden and Salmonella chincol by pulsed-field gel electrophoresis (PFGE) and enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR)

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