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Astragalus grahamianus extract: a novel source of bioactive compounds with antioxidant and neuroprotective activities

The Astragalus grahamianus (AG) Royle ex. Benth is traditionally used for the treatment of various human disorders. The current research work is aimed to explore the neuroprotective anti-Parkinson effects of various fractions of Astragalus grahamianus (A. grahamianus). Fine powder of Astragalus grah...

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Main Authors: Khan, M.W., Khan, R.A., Ahmad, M., Alkreathy, H.M., Mushtaq, N., Alam, O., Khan, M.I., Ullah, A., Khan, H.U., Haq, N.U., Khan, W.R.
Format: Article
Language:English
Published: Instituto Internacional de Ecologia 2024
Online Access:http://psasir.upm.edu.my/id/eprint/113921/1/113921.pdf
http://psasir.upm.edu.my/id/eprint/113921/
https://www.scielo.br/j/bjb/a/5ptBcLhhy9ykTkLqPH4xQbK/?lang=en
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spelling my.upm.eprints.1139212024-11-25T03:47:21Z http://psasir.upm.edu.my/id/eprint/113921/ Astragalus grahamianus extract: a novel source of bioactive compounds with antioxidant and neuroprotective activities Khan, M.W. Khan, R.A. Ahmad, M. Alkreathy, H.M. Mushtaq, N. Alam, O. Khan, M.I. Ullah, A. Khan, H.U. Haq, N.U. Khan, W.R. The Astragalus grahamianus (AG) Royle ex. Benth is traditionally used for the treatment of various human disorders. The current research work is aimed to explore the neuroprotective anti-Parkinson effects of various fractions of Astragalus grahamianus (A. grahamianus). Fine powder of Astragalus grahamianus was extracted with 70% methanol and then fractionated with various solvents on the basis of polarity. Standard protocols were used to investigate the bioactive constituents present in the various plant fractions. In-vitro antioxidant potential of various fractions was checked using diverse free radicals. In-vivo rats model was used to determined the neuroprotective effects of methanol fraction of A. grahamianus. The results revealed that various fractions of A. grahamianus contain flavonoids, cardiac glycosides, steroids, gums, terpenes, proteins, and carbohydrates except chloroform fraction lake the presence of steroids, cardiac glycosides, gums and saponins, aqueous fraction of steroids, terpenoids, gums and saponins, n-Hexane fraction steroids, carbohydrates, alkaloids, gums and flavonoids. The highest amount of total phenolic contents was found in AGME (32.67 ± 2.3 mg GAE/g). The AGME also showed enhanced free radicals cations potential against DPPH, ABTS and H2O2, respectively. The correlation between AOA (antioxidant activity) and TPC (total phenolic contents) revealed to be substantial. Relative R2 values for ABTS, H2O2, and DPPH activity are 0.9974, 0.9845, and 0.9678, respectively. The in-vivo neuroprotective activities showed significant results. Our findings highlight significant antioxidant, and neuroprotective possessions of AGME attributed to powerful bioactive compounds. Instituto Internacional de Ecologia 2024-05 Article PeerReviewed text en cc_by_4 http://psasir.upm.edu.my/id/eprint/113921/1/113921.pdf Khan, M.W. and Khan, R.A. and Ahmad, M. and Alkreathy, H.M. and Mushtaq, N. and Alam, O. and Khan, M.I. and Ullah, A. and Khan, H.U. and Haq, N.U. and Khan, W.R. (2024) Astragalus grahamianus extract: a novel source of bioactive compounds with antioxidant and neuroprotective activities. Brazilian Journal of Biology, 84. art. no. e281217. pp. 1-12. ISSN 1519-6984; eISSN: 1678-4375 https://www.scielo.br/j/bjb/a/5ptBcLhhy9ykTkLqPH4xQbK/?lang=en 10.1590/1519-6984.281217
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The Astragalus grahamianus (AG) Royle ex. Benth is traditionally used for the treatment of various human disorders. The current research work is aimed to explore the neuroprotective anti-Parkinson effects of various fractions of Astragalus grahamianus (A. grahamianus). Fine powder of Astragalus grahamianus was extracted with 70% methanol and then fractionated with various solvents on the basis of polarity. Standard protocols were used to investigate the bioactive constituents present in the various plant fractions. In-vitro antioxidant potential of various fractions was checked using diverse free radicals. In-vivo rats model was used to determined the neuroprotective effects of methanol fraction of A. grahamianus. The results revealed that various fractions of A. grahamianus contain flavonoids, cardiac glycosides, steroids, gums, terpenes, proteins, and carbohydrates except chloroform fraction lake the presence of steroids, cardiac glycosides, gums and saponins, aqueous fraction of steroids, terpenoids, gums and saponins, n-Hexane fraction steroids, carbohydrates, alkaloids, gums and flavonoids. The highest amount of total phenolic contents was found in AGME (32.67 ± 2.3 mg GAE/g). The AGME also showed enhanced free radicals cations potential against DPPH, ABTS and H2O2, respectively. The correlation between AOA (antioxidant activity) and TPC (total phenolic contents) revealed to be substantial. Relative R2 values for ABTS, H2O2, and DPPH activity are 0.9974, 0.9845, and 0.9678, respectively. The in-vivo neuroprotective activities showed significant results. Our findings highlight significant antioxidant, and neuroprotective possessions of AGME attributed to powerful bioactive compounds.
format Article
author Khan, M.W.
Khan, R.A.
Ahmad, M.
Alkreathy, H.M.
Mushtaq, N.
Alam, O.
Khan, M.I.
Ullah, A.
Khan, H.U.
Haq, N.U.
Khan, W.R.
spellingShingle Khan, M.W.
Khan, R.A.
Ahmad, M.
Alkreathy, H.M.
Mushtaq, N.
Alam, O.
Khan, M.I.
Ullah, A.
Khan, H.U.
Haq, N.U.
Khan, W.R.
Astragalus grahamianus extract: a novel source of bioactive compounds with antioxidant and neuroprotective activities
author_facet Khan, M.W.
Khan, R.A.
Ahmad, M.
Alkreathy, H.M.
Mushtaq, N.
Alam, O.
Khan, M.I.
Ullah, A.
Khan, H.U.
Haq, N.U.
Khan, W.R.
author_sort Khan, M.W.
title Astragalus grahamianus extract: a novel source of bioactive compounds with antioxidant and neuroprotective activities
title_short Astragalus grahamianus extract: a novel source of bioactive compounds with antioxidant and neuroprotective activities
title_full Astragalus grahamianus extract: a novel source of bioactive compounds with antioxidant and neuroprotective activities
title_fullStr Astragalus grahamianus extract: a novel source of bioactive compounds with antioxidant and neuroprotective activities
title_full_unstemmed Astragalus grahamianus extract: a novel source of bioactive compounds with antioxidant and neuroprotective activities
title_sort astragalus grahamianus extract: a novel source of bioactive compounds with antioxidant and neuroprotective activities
publisher Instituto Internacional de Ecologia
publishDate 2024
url http://psasir.upm.edu.my/id/eprint/113921/1/113921.pdf
http://psasir.upm.edu.my/id/eprint/113921/
https://www.scielo.br/j/bjb/a/5ptBcLhhy9ykTkLqPH4xQbK/?lang=en
_version_ 1817844663477862400
score 13.23648

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