Cover Image

Population Characterization of Planktonic Shrimp, Acetes japonicus (Decapoda: Sergestidae) using RAPD Technique

Genomic DNA of sergestid shrimp, Acetes japonicus was successfully extracted by using the Promega Wizard Genomic DNA Purification Kit. Population genetic characterization of A. japonicus along the western coast of Peninsular Malaysia (state of Perak, Malacca and Kedah) was examined using the Random...

Full description

Saved in:
Bibliographic Details
Main Authors: Deraman, Aziz, Siraj, Siti Shapor, Arshad, Aziz, Nurul Amin, Sanker Mohammad, Harmin, Sharr Azni
Format: Article
Language:English
English
Published: 2010
Online Access:http://psasir.upm.edu.my/id/eprint/11388/1/Population_Characterization_of_Planktonic_Shrimp.pdf
http://psasir.upm.edu.my/id/eprint/11388/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.11388
record_format eprints
spelling my.upm.eprints.113882013-05-27T07:48:49Z http://psasir.upm.edu.my/id/eprint/11388/ Population Characterization of Planktonic Shrimp, Acetes japonicus (Decapoda: Sergestidae) using RAPD Technique Deraman, Aziz Siraj, Siti Shapor Arshad, Aziz Nurul Amin, Sanker Mohammad Harmin, Sharr Azni Genomic DNA of sergestid shrimp, Acetes japonicus was successfully extracted by using the Promega Wizard Genomic DNA Purification Kit. Population genetic characterization of A. japonicus along the western coast of Peninsular Malaysia (state of Perak, Malacca and Kedah) was examined using the Random Amplified Polymorphic DNA (RAPD) marker. Twenty oligonucleotides from operon A kit were used to screen the populations, of which 6 were able to be amplified (OPA03, OPA04, OPA07, OPA09, OPA10, OPA16). The percentages of polymorphic bands of the three populations investigated varied from 57.77 to 87.77%. Genetic distances between populations and cluster analysis from UPGMA grouped the populations into two major clusters. The Perak and Malacca populations were in one cluster, while the Kedah population was clustered by itself, indicating a different population. The genetic distance was highest as 0.0999 for the Kedah and the Malacca populations while lowest value was 0.0413 for the Perak and the Malacca populations, which probably have a closed ancestral relationship. The results of this study suggested that RAPD analysis, if carried out carefully and accurately would give a good indication of the separation between individuals of different populations and is suitable for identification of closely related genotypes. 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/11388/1/Population_Characterization_of_Planktonic_Shrimp.pdf Deraman, Aziz and Siraj, Siti Shapor and Arshad, Aziz and Nurul Amin, Sanker Mohammad and Harmin, Sharr Azni (2010) Population Characterization of Planktonic Shrimp, Acetes japonicus (Decapoda: Sergestidae) using RAPD Technique. Journal of Biological Sciences, 10 (4). pp. 355-361. ISSN 1727-3048 10.3923/jbs.2010.355.361 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description Genomic DNA of sergestid shrimp, Acetes japonicus was successfully extracted by using the Promega Wizard Genomic DNA Purification Kit. Population genetic characterization of A. japonicus along the western coast of Peninsular Malaysia (state of Perak, Malacca and Kedah) was examined using the Random Amplified Polymorphic DNA (RAPD) marker. Twenty oligonucleotides from operon A kit were used to screen the populations, of which 6 were able to be amplified (OPA03, OPA04, OPA07, OPA09, OPA10, OPA16). The percentages of polymorphic bands of the three populations investigated varied from 57.77 to 87.77%. Genetic distances between populations and cluster analysis from UPGMA grouped the populations into two major clusters. The Perak and Malacca populations were in one cluster, while the Kedah population was clustered by itself, indicating a different population. The genetic distance was highest as 0.0999 for the Kedah and the Malacca populations while lowest value was 0.0413 for the Perak and the Malacca populations, which probably have a closed ancestral relationship. The results of this study suggested that RAPD analysis, if carried out carefully and accurately would give a good indication of the separation between individuals of different populations and is suitable for identification of closely related genotypes.
format Article
author Deraman, Aziz
Siraj, Siti Shapor
Arshad, Aziz
Nurul Amin, Sanker Mohammad
Harmin, Sharr Azni
spellingShingle Deraman, Aziz
Siraj, Siti Shapor
Arshad, Aziz
Nurul Amin, Sanker Mohammad
Harmin, Sharr Azni
Population Characterization of Planktonic Shrimp, Acetes japonicus (Decapoda: Sergestidae) using RAPD Technique
author_facet Deraman, Aziz
Siraj, Siti Shapor
Arshad, Aziz
Nurul Amin, Sanker Mohammad
Harmin, Sharr Azni
author_sort Deraman, Aziz
title Population Characterization of Planktonic Shrimp, Acetes japonicus (Decapoda: Sergestidae) using RAPD Technique
title_short Population Characterization of Planktonic Shrimp, Acetes japonicus (Decapoda: Sergestidae) using RAPD Technique
title_full Population Characterization of Planktonic Shrimp, Acetes japonicus (Decapoda: Sergestidae) using RAPD Technique
title_fullStr Population Characterization of Planktonic Shrimp, Acetes japonicus (Decapoda: Sergestidae) using RAPD Technique
title_full_unstemmed Population Characterization of Planktonic Shrimp, Acetes japonicus (Decapoda: Sergestidae) using RAPD Technique
title_sort population characterization of planktonic shrimp, acetes japonicus (decapoda: sergestidae) using rapd technique
publishDate 2010
url http://psasir.upm.edu.my/id/eprint/11388/1/Population_Characterization_of_Planktonic_Shrimp.pdf
http://psasir.upm.edu.my/id/eprint/11388/
_version_ 1643824811181342720
score 13.211869

Similar Items