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Evaluation of glutathione S-transferases expression as biomarkers by heavy metals in Geloina expansa from Sepang Besar River, Selangor, Malaysia

Glutathione S-transferases (GSTs) are enzymes involved in phase II of detoxification metabolism and could be used as biomarkers for water pollution. This study aims to determine heavy metal concentrations in the soft tissue of the mangrove clam Geloina expansa, as well as the expression of GSTs in t...

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Main Authors: Wakid, Sarini Ahmad, Abd Aziz, Nor Azwady Abd, Alias, Zazali, Mustafa, Muskhazli, Syazwan, Wan Mohd, Zulkifli, Syaizwan Zahmir
Format: Article
Language:English
Published: Universiti Putra Malaysia Press 2024
Online Access:http://psasir.upm.edu.my/id/eprint/113874/1/113874.pdf
http://psasir.upm.edu.my/id/eprint/113874/
http://www.pertanika.upm.edu.my/pjst/browse/regular-issue?article=JST-4439-2023
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spelling my.upm.eprints.1138742025-01-14T08:39:12Z http://psasir.upm.edu.my/id/eprint/113874/ Evaluation of glutathione S-transferases expression as biomarkers by heavy metals in Geloina expansa from Sepang Besar River, Selangor, Malaysia Wakid, Sarini Ahmad Abd Aziz, Nor Azwady Abd Alias, Zazali Mustafa, Muskhazli Syazwan, Wan Mohd Zulkifli, Syaizwan Zahmir Glutathione S-transferases (GSTs) are enzymes involved in phase II of detoxification metabolism and could be used as biomarkers for water pollution. This study aims to determine heavy metal concentrations in the soft tissue of the mangrove clam Geloina expansa, as well as the expression of GSTs in the species. The acid digestion method was used to digest the samples, followed by a standard USEPA 6010B procedure using inductively coupled plasma optical emission spectrometry (ICP-OES) to measure the heavy metal contents in the samples. GST enzyme activity was measured using 1-chloro-2, 4-dinitrobenzene (CDNB) as substrate. One-way ANOVA was performed to compare the mean values of heavy metal concentration, protein concentration, enzyme activity, and specific activity. There was a significant difference (p<0.05) for Zn, total protein, and specific activity in G. expansa, but no significant difference in Pb, Cu and enzyme activity. GST enzyme activities were estimated at 0.16 ± 0.01 µmol/min, with a protein content of 1.24 ± 0.04 mg. The specific activity for GST was 0.13 ± 0.01 µmol/ min/mg, calculated as the ratio of enzyme activity to the total protein. GST-specific activity positively correlates with Pb concentration in the soft tissue of G. expansa. Detailed studies on the effects of pollution on the expression of GST need to be further investigated for the future use of this species as an efficient biomarker model. Universiti Putra Malaysia Press 2024-07 Article PeerReviewed text en cc_by_nc_nd_4 http://psasir.upm.edu.my/id/eprint/113874/1/113874.pdf Wakid, Sarini Ahmad and Abd Aziz, Nor Azwady Abd and Alias, Zazali and Mustafa, Muskhazli and Syazwan, Wan Mohd and Zulkifli, Syaizwan Zahmir (2024) Evaluation of glutathione S-transferases expression as biomarkers by heavy metals in Geloina expansa from Sepang Besar River, Selangor, Malaysia. Pertanika Journal of Science and Technology, 32 (4). pp. 1439-1460. ISSN 0128-7680; eISSN: 2231-8526 http://www.pertanika.upm.edu.my/pjst/browse/regular-issue?article=JST-4439-2023 10.47836/pjst.32.4.01
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Glutathione S-transferases (GSTs) are enzymes involved in phase II of detoxification metabolism and could be used as biomarkers for water pollution. This study aims to determine heavy metal concentrations in the soft tissue of the mangrove clam Geloina expansa, as well as the expression of GSTs in the species. The acid digestion method was used to digest the samples, followed by a standard USEPA 6010B procedure using inductively coupled plasma optical emission spectrometry (ICP-OES) to measure the heavy metal contents in the samples. GST enzyme activity was measured using 1-chloro-2, 4-dinitrobenzene (CDNB) as substrate. One-way ANOVA was performed to compare the mean values of heavy metal concentration, protein concentration, enzyme activity, and specific activity. There was a significant difference (p<0.05) for Zn, total protein, and specific activity in G. expansa, but no significant difference in Pb, Cu and enzyme activity. GST enzyme activities were estimated at 0.16 ± 0.01 µmol/min, with a protein content of 1.24 ± 0.04 mg. The specific activity for GST was 0.13 ± 0.01 µmol/ min/mg, calculated as the ratio of enzyme activity to the total protein. GST-specific activity positively correlates with Pb concentration in the soft tissue of G. expansa. Detailed studies on the effects of pollution on the expression of GST need to be further investigated for the future use of this species as an efficient biomarker model.
format Article
author Wakid, Sarini Ahmad
Abd Aziz, Nor Azwady Abd
Alias, Zazali
Mustafa, Muskhazli
Syazwan, Wan Mohd
Zulkifli, Syaizwan Zahmir
spellingShingle Wakid, Sarini Ahmad
Abd Aziz, Nor Azwady Abd
Alias, Zazali
Mustafa, Muskhazli
Syazwan, Wan Mohd
Zulkifli, Syaizwan Zahmir
Evaluation of glutathione S-transferases expression as biomarkers by heavy metals in Geloina expansa from Sepang Besar River, Selangor, Malaysia
author_facet Wakid, Sarini Ahmad
Abd Aziz, Nor Azwady Abd
Alias, Zazali
Mustafa, Muskhazli
Syazwan, Wan Mohd
Zulkifli, Syaizwan Zahmir
author_sort Wakid, Sarini Ahmad
title Evaluation of glutathione S-transferases expression as biomarkers by heavy metals in Geloina expansa from Sepang Besar River, Selangor, Malaysia
title_short Evaluation of glutathione S-transferases expression as biomarkers by heavy metals in Geloina expansa from Sepang Besar River, Selangor, Malaysia
title_full Evaluation of glutathione S-transferases expression as biomarkers by heavy metals in Geloina expansa from Sepang Besar River, Selangor, Malaysia
title_fullStr Evaluation of glutathione S-transferases expression as biomarkers by heavy metals in Geloina expansa from Sepang Besar River, Selangor, Malaysia
title_full_unstemmed Evaluation of glutathione S-transferases expression as biomarkers by heavy metals in Geloina expansa from Sepang Besar River, Selangor, Malaysia
title_sort evaluation of glutathione s-transferases expression as biomarkers by heavy metals in geloina expansa from sepang besar river, selangor, malaysia
publisher Universiti Putra Malaysia Press
publishDate 2024
url http://psasir.upm.edu.my/id/eprint/113874/1/113874.pdf
http://psasir.upm.edu.my/id/eprint/113874/
http://www.pertanika.upm.edu.my/pjst/browse/regular-issue?article=JST-4439-2023
_version_ 1823093081344311296
score 13.239859

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