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Isolation, characterization and identification of plant growth-promoting rhizobacteria from the rhizosphere of Acacia mangium at sandy BRIS soil

This study has isolated, characterized, and identified potential plant growth-promoting rhizobacteria (PGPR) with multiple PGP characteristics (N2-fixation, P- and K-solubilization, IAA, and siderophores production) from the rhizosphere BRIS soil of Acacia mangium. A total of 24 pure colonies were i...

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Main Authors: Mustapha, Zakiah, Othman, Radziah, Nik Muhammad Nasir, Nik Nurnaeimah, Zawawi, Dhiya Dalila
Format: Article
Language:English
Published: Higher Education Press Limited Company 2024
Online Access:http://psasir.upm.edu.my/id/eprint/113861/1/113861.pdf
http://psasir.upm.edu.my/id/eprint/113861/
https://journal.hep.com.cn/fase/EN/10.15302/J-FASE-2024559
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spelling my.upm.eprints.1138612025-01-31T03:37:37Z http://psasir.upm.edu.my/id/eprint/113861/ Isolation, characterization and identification of plant growth-promoting rhizobacteria from the rhizosphere of Acacia mangium at sandy BRIS soil Mustapha, Zakiah Othman, Radziah Nik Muhammad Nasir, Nik Nurnaeimah Zawawi, Dhiya Dalila This study has isolated, characterized, and identified potential plant growth-promoting rhizobacteria (PGPR) with multiple PGP characteristics (N2-fixation, P- and K-solubilization, IAA, and siderophores production) from the rhizosphere BRIS soil of Acacia mangium. A total of 24 pure colonies were isolated and only 8 colonies were selected for further evaluation of the growth rate in 5% organic molasses medium supplemented with 2% KNO3. Based on the biochemical, potential PGP characteristics and growth performance, 3 superior PGPR strains were selected and identified as Paraburkholderia unamae (UA1), Bacillus amyloliquefaciens (UA6), and Enterobacter asburiae (UAA2) by partial sequencing of the 16S rRNA gene. The selected bacterial strains either in single or mixed (UA1 + UA6 + UAA2) cultures have shown a significant biochemical estimation of the PGP characteristics. Each strain has its own PGPR traits superiority with UA1 showing the best PGP characteristic followed by UA6 and UAA2. The use of mixed bacterial strains was beneficial as it showed the best performance in N2-fixation, siderophores production, and significant effect on corn phenology, growth and yield compared to using a single strain. These types of microbes showed potential to be used as biofertilizer and should be exploited more. Higher Education Press Limited Company 2024 Article PeerReviewed text en cc_by_4 http://psasir.upm.edu.my/id/eprint/113861/1/113861.pdf Mustapha, Zakiah and Othman, Radziah and Nik Muhammad Nasir, Nik Nurnaeimah and Zawawi, Dhiya Dalila (2024) Isolation, characterization and identification of plant growth-promoting rhizobacteria from the rhizosphere of Acacia mangium at sandy BRIS soil. Frontiers of Agricultural Science and Engineering, 11 (3). pp. 499-513. ISSN 2095-7505; eISSN: 2095-977X https://journal.hep.com.cn/fase/EN/10.15302/J-FASE-2024559 10.15302/J-FASE-2024559
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description This study has isolated, characterized, and identified potential plant growth-promoting rhizobacteria (PGPR) with multiple PGP characteristics (N2-fixation, P- and K-solubilization, IAA, and siderophores production) from the rhizosphere BRIS soil of Acacia mangium. A total of 24 pure colonies were isolated and only 8 colonies were selected for further evaluation of the growth rate in 5% organic molasses medium supplemented with 2% KNO3. Based on the biochemical, potential PGP characteristics and growth performance, 3 superior PGPR strains were selected and identified as Paraburkholderia unamae (UA1), Bacillus amyloliquefaciens (UA6), and Enterobacter asburiae (UAA2) by partial sequencing of the 16S rRNA gene. The selected bacterial strains either in single or mixed (UA1 + UA6 + UAA2) cultures have shown a significant biochemical estimation of the PGP characteristics. Each strain has its own PGPR traits superiority with UA1 showing the best PGP characteristic followed by UA6 and UAA2. The use of mixed bacterial strains was beneficial as it showed the best performance in N2-fixation, siderophores production, and significant effect on corn phenology, growth and yield compared to using a single strain. These types of microbes showed potential to be used as biofertilizer and should be exploited more.
format Article
author Mustapha, Zakiah
Othman, Radziah
Nik Muhammad Nasir, Nik Nurnaeimah
Zawawi, Dhiya Dalila
spellingShingle Mustapha, Zakiah
Othman, Radziah
Nik Muhammad Nasir, Nik Nurnaeimah
Zawawi, Dhiya Dalila
Isolation, characterization and identification of plant growth-promoting rhizobacteria from the rhizosphere of Acacia mangium at sandy BRIS soil
author_facet Mustapha, Zakiah
Othman, Radziah
Nik Muhammad Nasir, Nik Nurnaeimah
Zawawi, Dhiya Dalila
author_sort Mustapha, Zakiah
title Isolation, characterization and identification of plant growth-promoting rhizobacteria from the rhizosphere of Acacia mangium at sandy BRIS soil
title_short Isolation, characterization and identification of plant growth-promoting rhizobacteria from the rhizosphere of Acacia mangium at sandy BRIS soil
title_full Isolation, characterization and identification of plant growth-promoting rhizobacteria from the rhizosphere of Acacia mangium at sandy BRIS soil
title_fullStr Isolation, characterization and identification of plant growth-promoting rhizobacteria from the rhizosphere of Acacia mangium at sandy BRIS soil
title_full_unstemmed Isolation, characterization and identification of plant growth-promoting rhizobacteria from the rhizosphere of Acacia mangium at sandy BRIS soil
title_sort isolation, characterization and identification of plant growth-promoting rhizobacteria from the rhizosphere of acacia mangium at sandy bris soil
publisher Higher Education Press Limited Company
publishDate 2024
url http://psasir.upm.edu.my/id/eprint/113861/1/113861.pdf
http://psasir.upm.edu.my/id/eprint/113861/
https://journal.hep.com.cn/fase/EN/10.15302/J-FASE-2024559
_version_ 1823093080338726912
score 13.244413

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