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The effect of the molecular weight of blackberry polysaccharides on gut microbiota modulation and hypoglycemic effect in vivo

Blackberry polysaccharides with certain molecular weight distribution have good bioactivity. In this research, type 2 diabetes mice were used to investigate the hypoglycemic effect of blackberry polysaccharides with three different molecular weights, BBP (603.59 kDa), BBP-8 (408.13 kDa) and BBP-24 (...

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Main Authors: Xi, Lai, Weibing, Xu, Shuyong, Fu, Sheng-Hua, Li, Xiong, Fu, Chin-Ping, Tan, Ping-Ping, Wang, Zu-Man, Dou, Chun, Chen
Format: Article
Published: Royal Society of Chemistry 2024
Online Access:http://psasir.upm.edu.my/id/eprint/113837/
https://pubs.rsc.org/en/content/articlelanding/2024/fo/d4fo01989j
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spelling my.upm.eprints.1138372025-02-10T07:30:15Z http://psasir.upm.edu.my/id/eprint/113837/ The effect of the molecular weight of blackberry polysaccharides on gut microbiota modulation and hypoglycemic effect in vivo Xi, Lai Weibing, Xu Shuyong, Fu Sheng-Hua, Li Xiong, Fu Chin-Ping, Tan Ping-Ping, Wang Zu-Man, Dou Chun, Chen Blackberry polysaccharides with certain molecular weight distribution have good bioactivity. In this research, type 2 diabetes mice were used to investigate the hypoglycemic effect of blackberry polysaccharides with three different molecular weights, BBP (603.59 kDa), BBP-8 (408.13 kDa) and BBP-24 (247.62 kDa), through gut microbiota modulation. Blackberry polysaccharides exhibited stronger hypoglycemic activity after degradation, and the FBG of BBP, BBP-8 and BBP-24 was reduced to 20.21 ± 4.17 mmol L−1, 20.6 ± 7.23 mmol L−1 and 17.32 ± 6.59 mmol L−1 and OGTT-AUC was reduced by 14.76%, 19.80% and 25.04%, respectively, after 8-week intervention. Furthermore, 16S rRNA gene sequencing analysis indicated that BBP, BBP-8 and BBP-24 could reshape the diversity and composition of the gut microbiota. From 0 to 4 weeks, the F/B of BBP, BBP-8 and BBP-24 reduced by 56.44%, 47.19% and 62.04%, reaching 3.39, 6.54, and 3.11 in the 8th week, respectively, which suggested the faster utilization of BBP-24. Moreover, the intervention the three blackberry polysaccharides increased the relative abundance of the targeted beneficial bacteria Oscillospira and Bacteroidaceae Bacteroides and decreased the relative abundance of the pathogenic bacterium Allobaculum. In general, the result demonstrated that blackberry polysaccharides with a lower molecular weight are more easily fermented, making the theoretical basis for the development of blackberry polysaccharides as a probiotic food to rapidly regulate intestinal flora for type 2 diabetes. Royal Society of Chemistry 2024-07 Article PeerReviewed Xi, Lai and Weibing, Xu and Shuyong, Fu and Sheng-Hua, Li and Xiong, Fu and Chin-Ping, Tan and Ping-Ping, Wang and Zu-Man, Dou and Chun, Chen (2024) The effect of the molecular weight of blackberry polysaccharides on gut microbiota modulation and hypoglycemic effect in vivo. Food and Function, 15 (16). pp. 8586-8603. ISSN 2042-6496; eISSN: 2042-650X https://pubs.rsc.org/en/content/articlelanding/2024/fo/d4fo01989j 10.1039/d4fo01989j
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description Blackberry polysaccharides with certain molecular weight distribution have good bioactivity. In this research, type 2 diabetes mice were used to investigate the hypoglycemic effect of blackberry polysaccharides with three different molecular weights, BBP (603.59 kDa), BBP-8 (408.13 kDa) and BBP-24 (247.62 kDa), through gut microbiota modulation. Blackberry polysaccharides exhibited stronger hypoglycemic activity after degradation, and the FBG of BBP, BBP-8 and BBP-24 was reduced to 20.21 ± 4.17 mmol L−1, 20.6 ± 7.23 mmol L−1 and 17.32 ± 6.59 mmol L−1 and OGTT-AUC was reduced by 14.76%, 19.80% and 25.04%, respectively, after 8-week intervention. Furthermore, 16S rRNA gene sequencing analysis indicated that BBP, BBP-8 and BBP-24 could reshape the diversity and composition of the gut microbiota. From 0 to 4 weeks, the F/B of BBP, BBP-8 and BBP-24 reduced by 56.44%, 47.19% and 62.04%, reaching 3.39, 6.54, and 3.11 in the 8th week, respectively, which suggested the faster utilization of BBP-24. Moreover, the intervention the three blackberry polysaccharides increased the relative abundance of the targeted beneficial bacteria Oscillospira and Bacteroidaceae Bacteroides and decreased the relative abundance of the pathogenic bacterium Allobaculum. In general, the result demonstrated that blackberry polysaccharides with a lower molecular weight are more easily fermented, making the theoretical basis for the development of blackberry polysaccharides as a probiotic food to rapidly regulate intestinal flora for type 2 diabetes.
format Article
author Xi, Lai
Weibing, Xu
Shuyong, Fu
Sheng-Hua, Li
Xiong, Fu
Chin-Ping, Tan
Ping-Ping, Wang
Zu-Man, Dou
Chun, Chen
spellingShingle Xi, Lai
Weibing, Xu
Shuyong, Fu
Sheng-Hua, Li
Xiong, Fu
Chin-Ping, Tan
Ping-Ping, Wang
Zu-Man, Dou
Chun, Chen
The effect of the molecular weight of blackberry polysaccharides on gut microbiota modulation and hypoglycemic effect in vivo
author_facet Xi, Lai
Weibing, Xu
Shuyong, Fu
Sheng-Hua, Li
Xiong, Fu
Chin-Ping, Tan
Ping-Ping, Wang
Zu-Man, Dou
Chun, Chen
author_sort Xi, Lai
title The effect of the molecular weight of blackberry polysaccharides on gut microbiota modulation and hypoglycemic effect in vivo
title_short The effect of the molecular weight of blackberry polysaccharides on gut microbiota modulation and hypoglycemic effect in vivo
title_full The effect of the molecular weight of blackberry polysaccharides on gut microbiota modulation and hypoglycemic effect in vivo
title_fullStr The effect of the molecular weight of blackberry polysaccharides on gut microbiota modulation and hypoglycemic effect in vivo
title_full_unstemmed The effect of the molecular weight of blackberry polysaccharides on gut microbiota modulation and hypoglycemic effect in vivo
title_sort effect of the molecular weight of blackberry polysaccharides on gut microbiota modulation and hypoglycemic effect in vivo
publisher Royal Society of Chemistry
publishDate 2024
url http://psasir.upm.edu.my/id/eprint/113837/
https://pubs.rsc.org/en/content/articlelanding/2024/fo/d4fo01989j
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score 13.239859

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