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Antibacterial activity of free and liposome-encapsulated Tylosin against planktonic and biofilm forms of Corynebacterium pseudotuberculosis isolated from goats

Corynebacterium pseudotuberculosis causes infection in goats, sheep, cattle, and humans. The disease imposing huge economic losses such as reducing milk, meat, and wool production and downgrading and condemnation of affected carcass to goat-rearing industry. Despite all its devastating effects, no e...

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Main Authors: Saddiqi, Mohammad Ehsan, Kadir, Arifah Abdul
Format: Article
Language:English
Published: Istanbul University 2024
Online Access:http://psasir.upm.edu.my/id/eprint/113554/1/113554.pdf
http://psasir.upm.edu.my/id/eprint/113554/
https://actavet.org/en/antibacterial-activity-of-free-and-liposome-encapsulated-tylosin-against-planktonic-and-biofilm-forms-of-corynebacterium-pseudotuberculosis-isolated-from-goats-13650
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spelling my.upm.eprints.1135542024-11-26T04:18:15Z http://psasir.upm.edu.my/id/eprint/113554/ Antibacterial activity of free and liposome-encapsulated Tylosin against planktonic and biofilm forms of Corynebacterium pseudotuberculosis isolated from goats Saddiqi, Mohammad Ehsan Kadir, Arifah Abdul Corynebacterium pseudotuberculosis causes infection in goats, sheep, cattle, and humans. The disease imposing huge economic losses such as reducing milk, meat, and wool production and downgrading and condemnation of affected carcass to goat-rearing industry. Despite all its devastating effects, no effective antimicrobial was suggested for the treatment of this pathogen due to its highly resistant nature. Development and utilization of nanoparticles has paved the way to revive the efficacy of conventional antibiotics. Encapsulation of antibiotics within liposome as a nanoparticle may present a potential approach for successful treatment of resistant bacterial infections. The aim of this research was therefore to evaluate the antibacterial activity of free and liposome-encapsulating tylosin against Corynebacterium pseudotuberculosis. The minimum inhibitory concentration of free and liposome-encapsulated tylosin was determined by microbroth dilution and resazurin colorimetric-based methods. The antibiofilm activity of free and liposomal tylosin was assessed by crystal violet and resazurin techniques. Acridine orange/ethidium bromide staining assay was performed to assess the effect of free and liposomal tylosin on pre-formed biofilm of Corynebacterium pseudotuberculosis. The findings demonstrated minimum inhibitory concentration value of 1 μg/mL and 32 μg/mL for free and liposomal tylosin, respectively. The biofilm inhibitory data showed that free tylosin inhibited the formation of Corynebacterium pseudotuberculosis at 2 μg/mL, while this concentration was 64 μg/mL for liposomal tylosin. The antibiofilm concentration was 1024 μg/mL and 2048 μg/mL for liposome-encapsulated and free tylosin, respectively. It can be concluded that liposome as a nanocarrier can offer an excellent system of drug delivery for enhancing the efficacy of antibiotics against resistant form of Corynebacterium pseudotuberculosis. Istanbul University 2024 Article PeerReviewed text en cc_by_nc_4 http://psasir.upm.edu.my/id/eprint/113554/1/113554.pdf Saddiqi, Mohammad Ehsan and Kadir, Arifah Abdul (2024) Antibacterial activity of free and liposome-encapsulated Tylosin against planktonic and biofilm forms of Corynebacterium pseudotuberculosis isolated from goats. Acta Veterinaria Eurasia, 50 (2). pp. 119-127. ISSN 2619-905X; eISSN: 2619-905X https://actavet.org/en/antibacterial-activity-of-free-and-liposome-encapsulated-tylosin-against-planktonic-and-biofilm-forms-of-corynebacterium-pseudotuberculosis-isolated-from-goats-13650 10.5152/actavet.2024.23070
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Corynebacterium pseudotuberculosis causes infection in goats, sheep, cattle, and humans. The disease imposing huge economic losses such as reducing milk, meat, and wool production and downgrading and condemnation of affected carcass to goat-rearing industry. Despite all its devastating effects, no effective antimicrobial was suggested for the treatment of this pathogen due to its highly resistant nature. Development and utilization of nanoparticles has paved the way to revive the efficacy of conventional antibiotics. Encapsulation of antibiotics within liposome as a nanoparticle may present a potential approach for successful treatment of resistant bacterial infections. The aim of this research was therefore to evaluate the antibacterial activity of free and liposome-encapsulating tylosin against Corynebacterium pseudotuberculosis. The minimum inhibitory concentration of free and liposome-encapsulated tylosin was determined by microbroth dilution and resazurin colorimetric-based methods. The antibiofilm activity of free and liposomal tylosin was assessed by crystal violet and resazurin techniques. Acridine orange/ethidium bromide staining assay was performed to assess the effect of free and liposomal tylosin on pre-formed biofilm of Corynebacterium pseudotuberculosis. The findings demonstrated minimum inhibitory concentration value of 1 μg/mL and 32 μg/mL for free and liposomal tylosin, respectively. The biofilm inhibitory data showed that free tylosin inhibited the formation of Corynebacterium pseudotuberculosis at 2 μg/mL, while this concentration was 64 μg/mL for liposomal tylosin. The antibiofilm concentration was 1024 μg/mL and 2048 μg/mL for liposome-encapsulated and free tylosin, respectively. It can be concluded that liposome as a nanocarrier can offer an excellent system of drug delivery for enhancing the efficacy of antibiotics against resistant form of Corynebacterium pseudotuberculosis.
format Article
author Saddiqi, Mohammad Ehsan
Kadir, Arifah Abdul
spellingShingle Saddiqi, Mohammad Ehsan
Kadir, Arifah Abdul
Antibacterial activity of free and liposome-encapsulated Tylosin against planktonic and biofilm forms of Corynebacterium pseudotuberculosis isolated from goats
author_facet Saddiqi, Mohammad Ehsan
Kadir, Arifah Abdul
author_sort Saddiqi, Mohammad Ehsan
title Antibacterial activity of free and liposome-encapsulated Tylosin against planktonic and biofilm forms of Corynebacterium pseudotuberculosis isolated from goats
title_short Antibacterial activity of free and liposome-encapsulated Tylosin against planktonic and biofilm forms of Corynebacterium pseudotuberculosis isolated from goats
title_full Antibacterial activity of free and liposome-encapsulated Tylosin against planktonic and biofilm forms of Corynebacterium pseudotuberculosis isolated from goats
title_fullStr Antibacterial activity of free and liposome-encapsulated Tylosin against planktonic and biofilm forms of Corynebacterium pseudotuberculosis isolated from goats
title_full_unstemmed Antibacterial activity of free and liposome-encapsulated Tylosin against planktonic and biofilm forms of Corynebacterium pseudotuberculosis isolated from goats
title_sort antibacterial activity of free and liposome-encapsulated tylosin against planktonic and biofilm forms of corynebacterium pseudotuberculosis isolated from goats
publisher Istanbul University
publishDate 2024
url http://psasir.upm.edu.my/id/eprint/113554/1/113554.pdf
http://psasir.upm.edu.my/id/eprint/113554/
https://actavet.org/en/antibacterial-activity-of-free-and-liposome-encapsulated-tylosin-against-planktonic-and-biofilm-forms-of-corynebacterium-pseudotuberculosis-isolated-from-goats-13650
_version_ 1817844676997152768
score 13.244413

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