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Identification of skin bacterial profiles of early deceased bodies and the rto post-mortem interval

Post-mortem microbiology (PMM) is an important tool in identifying possible causes of sudden unexpected death, as an infectious cause is highly suspected. However, contamination is a major problem in microbiology, and this has increased the difficulty determining the true pathogen that contributes t...

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Main Authors: Chong, C.K., Emamjomeh, M., Joseph, N., Siew, S.F., Maeda, T., Mustapha, N.A., Hoshiko, Y., Muthanna, A., Amin-Nordin, S.
Format: Article
Language:English
Published: Malaysian Society for Parasitology 2024
Online Access:http://psasir.upm.edu.my/id/eprint/113537/1/113537.pdf
http://psasir.upm.edu.my/id/eprint/113537/
https://msptm.org/files/Vol41No1/tb-41-1-013-Chong-C-K.pdf
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spelling my.upm.eprints.1135372024-11-26T03:47:25Z http://psasir.upm.edu.my/id/eprint/113537/ Identification of skin bacterial profiles of early deceased bodies and the rto post-mortem interval Chong, C.K. Emamjomeh, M. Joseph, N. Siew, S.F. Maeda, T. Mustapha, N.A. Hoshiko, Y. Muthanna, A. Amin-Nordin, S. Post-mortem microbiology (PMM) is an important tool in identifying possible causes of sudden unexpected death, as an infectious cause is highly suspected. However, contamination is a major problem in microbiology, and this has increased the difficulty determining the true pathogen that contributes to death in post-mortem cases. Skin commensals are common contaminants in blood cultures. This study was conducted to investigate the skin flora on early deceased bodies and observe the bacteria detected at different post-mortem intervals (PMIs). As blood is usually drawn from the neck and femoral sites for PMM examination, the two body sites were chosen as the sampling sites. Skin swab samples from the neck and femoral (n=80) of each early deceased body were collected by sterile cotton swabs. DNA was extracted from the swabs and then subjected to high-throughput 16S rRNA sequencing by using the Illumina MiSeq platform. Staphylococcus was found to be the most dominant genus in both neck and femoral sites. LEfSe results showed that Cutibacterium is significantly different at the neck site while Corynebacterium is more abundant at femoral site. There are significant differences at genus level between PMI<5H and PMI>5H at both neck and femoral sites. The findings of the present study may act as a reference for microbiologists and forensic pathologists when mixed growth or contamination occurs in post-mortem blood cultures. Malaysian Society for Parasitology 2024 Article PeerReviewed text en cc_by_nc_4 http://psasir.upm.edu.my/id/eprint/113537/1/113537.pdf Chong, C.K. and Emamjomeh, M. and Joseph, N. and Siew, S.F. and Maeda, T. and Mustapha, N.A. and Hoshiko, Y. and Muthanna, A. and Amin-Nordin, S. (2024) Identification of skin bacterial profiles of early deceased bodies and the rto post-mortem interval. Tropical Biomedicine, 41 (1). pp. 109-117. ISSN 0127-5720; eISSN: 2521-9855 https://msptm.org/files/Vol41No1/tb-41-1-013-Chong-C-K.pdf 10.47665/tb.41.1.013
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Post-mortem microbiology (PMM) is an important tool in identifying possible causes of sudden unexpected death, as an infectious cause is highly suspected. However, contamination is a major problem in microbiology, and this has increased the difficulty determining the true pathogen that contributes to death in post-mortem cases. Skin commensals are common contaminants in blood cultures. This study was conducted to investigate the skin flora on early deceased bodies and observe the bacteria detected at different post-mortem intervals (PMIs). As blood is usually drawn from the neck and femoral sites for PMM examination, the two body sites were chosen as the sampling sites. Skin swab samples from the neck and femoral (n=80) of each early deceased body were collected by sterile cotton swabs. DNA was extracted from the swabs and then subjected to high-throughput 16S rRNA sequencing by using the Illumina MiSeq platform. Staphylococcus was found to be the most dominant genus in both neck and femoral sites. LEfSe results showed that Cutibacterium is significantly different at the neck site while Corynebacterium is more abundant at femoral site. There are significant differences at genus level between PMI<5H and PMI>5H at both neck and femoral sites. The findings of the present study may act as a reference for microbiologists and forensic pathologists when mixed growth or contamination occurs in post-mortem blood cultures.
format Article
author Chong, C.K.
Emamjomeh, M.
Joseph, N.
Siew, S.F.
Maeda, T.
Mustapha, N.A.
Hoshiko, Y.
Muthanna, A.
Amin-Nordin, S.
spellingShingle Chong, C.K.
Emamjomeh, M.
Joseph, N.
Siew, S.F.
Maeda, T.
Mustapha, N.A.
Hoshiko, Y.
Muthanna, A.
Amin-Nordin, S.
Identification of skin bacterial profiles of early deceased bodies and the rto post-mortem interval
author_facet Chong, C.K.
Emamjomeh, M.
Joseph, N.
Siew, S.F.
Maeda, T.
Mustapha, N.A.
Hoshiko, Y.
Muthanna, A.
Amin-Nordin, S.
author_sort Chong, C.K.
title Identification of skin bacterial profiles of early deceased bodies and the rto post-mortem interval
title_short Identification of skin bacterial profiles of early deceased bodies and the rto post-mortem interval
title_full Identification of skin bacterial profiles of early deceased bodies and the rto post-mortem interval
title_fullStr Identification of skin bacterial profiles of early deceased bodies and the rto post-mortem interval
title_full_unstemmed Identification of skin bacterial profiles of early deceased bodies and the rto post-mortem interval
title_sort identification of skin bacterial profiles of early deceased bodies and the rto post-mortem interval
publisher Malaysian Society for Parasitology
publishDate 2024
url http://psasir.upm.edu.my/id/eprint/113537/1/113537.pdf
http://psasir.upm.edu.my/id/eprint/113537/
https://msptm.org/files/Vol41No1/tb-41-1-013-Chong-C-K.pdf
_version_ 1817844674532999168
score 13.223943

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