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Epidemiology of rumen fluke infection in selected buffalo farms in perak, malaysia: prevalence, molecular species identification, and associated risk factors

Rumen flukes cause heavy economic losses in the ruminant industry worldwide, especially in tropical and subtropical countries. This study estimated the prevalence of rumen flukes in buffaloes, identified the species diversity, and determined risk factors associated with rumen fluke prevalence in Per...

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Main Authors: Ahmad Tookhy, Nazir, Md Isa, Nur-Mahiza, Abd Rahaman, Yasmin, Ahmad, Nur Indah, Sharma, Reuben Sunil Kumar, Idris, Lokman Hakim, Mansor, Rozaihan, Bui, Dung Thi, Hamzah, Noor Hazfalinda, Shakhes, Shoaib Ahmad
Format: Article
Language:English
Published: Springer Science and Business Media Deutschland 2024
Online Access:http://psasir.upm.edu.my/id/eprint/113510/1/113510.pdf
http://psasir.upm.edu.my/id/eprint/113510/
https://link.springer.com/article/10.1007/s00436-024-08219-9
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spelling my.upm.eprints.1135102024-11-26T02:41:16Z http://psasir.upm.edu.my/id/eprint/113510/ Epidemiology of rumen fluke infection in selected buffalo farms in perak, malaysia: prevalence, molecular species identification, and associated risk factors Ahmad Tookhy, Nazir Md Isa, Nur-Mahiza Abd Rahaman, Yasmin Ahmad, Nur Indah Sharma, Reuben Sunil Kumar Idris, Lokman Hakim Mansor, Rozaihan Bui, Dung Thi Hamzah, Noor Hazfalinda Shakhes, Shoaib Ahmad Rumen flukes cause heavy economic losses in the ruminant industry worldwide, especially in tropical and subtropical countries. This study estimated the prevalence of rumen flukes in buffaloes, identified the species diversity, and determined risk factors associated with rumen fluke prevalence in Perak, Peninsular Malaysia. A cross-sectional study was conducted, and 321 faecal samples were collected from six buffalo farms. A structured questionnaire was developed, and farmers were interviewed to obtain information regarding risk factors associated with rumen fluke infection. The faecal samples were examined using sedimentation and Flukefinder® techniques. Genomic DNA was extracted from the fluke eggs recovered using the Flukefinder® method, and the internal transcribed spacer 2 (ITS2) fragment was amplified and sequenced to facilitate species identification. The results showed that the overall prevalence of rumen fluke across the sampled farms was 40.2% (129/321). Three rumen fluke species were identified, namely, Fischoederius elongatus, F. cobboldi, and Orthocoelium streptocoelium. Several management factors had a significant association (P < 0.05) with rumen fluke prevalence, including production type, cleaning of the stable, drinking water system, flooding around the farm, grazing system, pasture sharing with other livestock, and deworming program. This work constitutes the first attempt to understand the epidemiology of rumen fluke infection in the region and suggests that good farm management, pasture management, choosing appropriate drugs, and proper husbandry practices may improve buffalo health and production in areas where rumen flukes are prevalent. Springer Science and Business Media Deutschland 2024 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/113510/1/113510.pdf Ahmad Tookhy, Nazir and Md Isa, Nur-Mahiza and Abd Rahaman, Yasmin and Ahmad, Nur Indah and Sharma, Reuben Sunil Kumar and Idris, Lokman Hakim and Mansor, Rozaihan and Bui, Dung Thi and Hamzah, Noor Hazfalinda and Shakhes, Shoaib Ahmad (2024) Epidemiology of rumen fluke infection in selected buffalo farms in perak, malaysia: prevalence, molecular species identification, and associated risk factors. Parasitology Research, 123 (5). art. no. 199. pp. 1-11. ISSN 0932-0113; eISSN: 1432-1955 https://link.springer.com/article/10.1007/s00436-024-08219-9 10.1007/s00436-024-08219-9
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Rumen flukes cause heavy economic losses in the ruminant industry worldwide, especially in tropical and subtropical countries. This study estimated the prevalence of rumen flukes in buffaloes, identified the species diversity, and determined risk factors associated with rumen fluke prevalence in Perak, Peninsular Malaysia. A cross-sectional study was conducted, and 321 faecal samples were collected from six buffalo farms. A structured questionnaire was developed, and farmers were interviewed to obtain information regarding risk factors associated with rumen fluke infection. The faecal samples were examined using sedimentation and Flukefinder® techniques. Genomic DNA was extracted from the fluke eggs recovered using the Flukefinder® method, and the internal transcribed spacer 2 (ITS2) fragment was amplified and sequenced to facilitate species identification. The results showed that the overall prevalence of rumen fluke across the sampled farms was 40.2% (129/321). Three rumen fluke species were identified, namely, Fischoederius elongatus, F. cobboldi, and Orthocoelium streptocoelium. Several management factors had a significant association (P < 0.05) with rumen fluke prevalence, including production type, cleaning of the stable, drinking water system, flooding around the farm, grazing system, pasture sharing with other livestock, and deworming program. This work constitutes the first attempt to understand the epidemiology of rumen fluke infection in the region and suggests that good farm management, pasture management, choosing appropriate drugs, and proper husbandry practices may improve buffalo health and production in areas where rumen flukes are prevalent.
format Article
author Ahmad Tookhy, Nazir
Md Isa, Nur-Mahiza
Abd Rahaman, Yasmin
Ahmad, Nur Indah
Sharma, Reuben Sunil Kumar
Idris, Lokman Hakim
Mansor, Rozaihan
Bui, Dung Thi
Hamzah, Noor Hazfalinda
Shakhes, Shoaib Ahmad
spellingShingle Ahmad Tookhy, Nazir
Md Isa, Nur-Mahiza
Abd Rahaman, Yasmin
Ahmad, Nur Indah
Sharma, Reuben Sunil Kumar
Idris, Lokman Hakim
Mansor, Rozaihan
Bui, Dung Thi
Hamzah, Noor Hazfalinda
Shakhes, Shoaib Ahmad
Epidemiology of rumen fluke infection in selected buffalo farms in perak, malaysia: prevalence, molecular species identification, and associated risk factors
author_facet Ahmad Tookhy, Nazir
Md Isa, Nur-Mahiza
Abd Rahaman, Yasmin
Ahmad, Nur Indah
Sharma, Reuben Sunil Kumar
Idris, Lokman Hakim
Mansor, Rozaihan
Bui, Dung Thi
Hamzah, Noor Hazfalinda
Shakhes, Shoaib Ahmad
author_sort Ahmad Tookhy, Nazir
title Epidemiology of rumen fluke infection in selected buffalo farms in perak, malaysia: prevalence, molecular species identification, and associated risk factors
title_short Epidemiology of rumen fluke infection in selected buffalo farms in perak, malaysia: prevalence, molecular species identification, and associated risk factors
title_full Epidemiology of rumen fluke infection in selected buffalo farms in perak, malaysia: prevalence, molecular species identification, and associated risk factors
title_fullStr Epidemiology of rumen fluke infection in selected buffalo farms in perak, malaysia: prevalence, molecular species identification, and associated risk factors
title_full_unstemmed Epidemiology of rumen fluke infection in selected buffalo farms in perak, malaysia: prevalence, molecular species identification, and associated risk factors
title_sort epidemiology of rumen fluke infection in selected buffalo farms in perak, malaysia: prevalence, molecular species identification, and associated risk factors
publisher Springer Science and Business Media Deutschland
publishDate 2024
url http://psasir.upm.edu.my/id/eprint/113510/1/113510.pdf
http://psasir.upm.edu.my/id/eprint/113510/
https://link.springer.com/article/10.1007/s00436-024-08219-9
_version_ 1817844670902829056
score 13.223943

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