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Genome mining of Lactiplantibacillus plantarum PA21: insights into its antimicrobial potential

Background: The dramatic increase of antimicrobial resistance in the healthcare realm has become inexorably linked to the abuse of antibiotics over the years. Therefore, this study seeks to identify potential postbiotic metabolites derived from lactic acid bacteria such as Lactiplantibacillus planta...

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Main Authors: Isaac, Sharleen Livina, Abdul Malek, Ahmad Zuhairi, Hazif, Nurul Syafika, Roslan, Farah Syahrain, Mohd Hashim, Amalia, Song, Adelene Ai-Lian, Abdul Rahim, Raha, Wan Nur Ismah, Wan Ahmad Kamil
Format: Article
Language:English
Published: BioMed Central 2024
Online Access:http://psasir.upm.edu.my/id/eprint/113472/1/113472.pdf
http://psasir.upm.edu.my/id/eprint/113472/
https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-024-10451-7
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spelling my.upm.eprints.1134722024-11-25T07:06:46Z http://psasir.upm.edu.my/id/eprint/113472/ Genome mining of Lactiplantibacillus plantarum PA21: insights into its antimicrobial potential Isaac, Sharleen Livina Abdul Malek, Ahmad Zuhairi Hazif, Nurul Syafika Roslan, Farah Syahrain Mohd Hashim, Amalia Song, Adelene Ai-Lian Abdul Rahim, Raha Wan Nur Ismah, Wan Ahmad Kamil Background: The dramatic increase of antimicrobial resistance in the healthcare realm has become inexorably linked to the abuse of antibiotics over the years. Therefore, this study seeks to identify potential postbiotic metabolites derived from lactic acid bacteria such as Lactiplantibacillus plantarum that could exhibit antimicrobial properties against multi-drug resistant pathogens. Results: In the present work, the genome sequence of Lactiplantibacillus plantarum PA21 consisting of three contigs was assembled to a size of 3,218,706 bp. Phylogenomic analysis and average nucleotide identity (ANI) revealed L. plantarum PA21 is closely related to genomes isolated from diverse niches such as dairy products, food, and animals. Genome mining through the BAGEL4 and antiSMASH database revealed four bacteriocins in a single cluster and four regions of biosynthetic gene clusters responsible for the production of bioactive compounds. The potential probiotic genes indirectly responsible for postbiotic metabolites production were also identified. Additionally, in vitro studies showed that the L. plantarum PA21 cell-free supernatant exhibited antimicrobial activity against all nine methicillin-resistant Staphylococcus aureus (MRSA) and three out of 13 Klebsiella pneumoniae clinical isolates tested. Conclusion: Results in this study demonstrates that L. plantarum PA21 postbiotic metabolites is a prolific source of antimicrobials against multi-drug resistant pathogens with potential antimicrobial properties. BioMed Central 2024 Article PeerReviewed text en cc_by_4 http://psasir.upm.edu.my/id/eprint/113472/1/113472.pdf Isaac, Sharleen Livina and Abdul Malek, Ahmad Zuhairi and Hazif, Nurul Syafika and Roslan, Farah Syahrain and Mohd Hashim, Amalia and Song, Adelene Ai-Lian and Abdul Rahim, Raha and Wan Nur Ismah, Wan Ahmad Kamil (2024) Genome mining of Lactiplantibacillus plantarum PA21: insights into its antimicrobial potential. BMC Genomics, 25 (1). art. no. 571. pp. 1-13. ISSN 1471-2164; eISSN: 1471-2164 https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-024-10451-7 10.1186/s12864-024-10451-7
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Background: The dramatic increase of antimicrobial resistance in the healthcare realm has become inexorably linked to the abuse of antibiotics over the years. Therefore, this study seeks to identify potential postbiotic metabolites derived from lactic acid bacteria such as Lactiplantibacillus plantarum that could exhibit antimicrobial properties against multi-drug resistant pathogens. Results: In the present work, the genome sequence of Lactiplantibacillus plantarum PA21 consisting of three contigs was assembled to a size of 3,218,706 bp. Phylogenomic analysis and average nucleotide identity (ANI) revealed L. plantarum PA21 is closely related to genomes isolated from diverse niches such as dairy products, food, and animals. Genome mining through the BAGEL4 and antiSMASH database revealed four bacteriocins in a single cluster and four regions of biosynthetic gene clusters responsible for the production of bioactive compounds. The potential probiotic genes indirectly responsible for postbiotic metabolites production were also identified. Additionally, in vitro studies showed that the L. plantarum PA21 cell-free supernatant exhibited antimicrobial activity against all nine methicillin-resistant Staphylococcus aureus (MRSA) and three out of 13 Klebsiella pneumoniae clinical isolates tested. Conclusion: Results in this study demonstrates that L. plantarum PA21 postbiotic metabolites is a prolific source of antimicrobials against multi-drug resistant pathogens with potential antimicrobial properties.
format Article
author Isaac, Sharleen Livina
Abdul Malek, Ahmad Zuhairi
Hazif, Nurul Syafika
Roslan, Farah Syahrain
Mohd Hashim, Amalia
Song, Adelene Ai-Lian
Abdul Rahim, Raha
Wan Nur Ismah, Wan Ahmad Kamil
spellingShingle Isaac, Sharleen Livina
Abdul Malek, Ahmad Zuhairi
Hazif, Nurul Syafika
Roslan, Farah Syahrain
Mohd Hashim, Amalia
Song, Adelene Ai-Lian
Abdul Rahim, Raha
Wan Nur Ismah, Wan Ahmad Kamil
Genome mining of Lactiplantibacillus plantarum PA21: insights into its antimicrobial potential
author_facet Isaac, Sharleen Livina
Abdul Malek, Ahmad Zuhairi
Hazif, Nurul Syafika
Roslan, Farah Syahrain
Mohd Hashim, Amalia
Song, Adelene Ai-Lian
Abdul Rahim, Raha
Wan Nur Ismah, Wan Ahmad Kamil
author_sort Isaac, Sharleen Livina
title Genome mining of Lactiplantibacillus plantarum PA21: insights into its antimicrobial potential
title_short Genome mining of Lactiplantibacillus plantarum PA21: insights into its antimicrobial potential
title_full Genome mining of Lactiplantibacillus plantarum PA21: insights into its antimicrobial potential
title_fullStr Genome mining of Lactiplantibacillus plantarum PA21: insights into its antimicrobial potential
title_full_unstemmed Genome mining of Lactiplantibacillus plantarum PA21: insights into its antimicrobial potential
title_sort genome mining of lactiplantibacillus plantarum pa21: insights into its antimicrobial potential
publisher BioMed Central
publishDate 2024
url http://psasir.upm.edu.my/id/eprint/113472/1/113472.pdf
http://psasir.upm.edu.my/id/eprint/113472/
https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-024-10451-7
_version_ 1817844641848885248
score 13.222552

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