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Baeckea frutescens L. Promotes wound healing by upregulating expression of TGF-β, IL-1 β, VEGF and MMP-2

Baeckea frutescens L. has been traditionally used for treating snakebites and is known to possess antifebrile and hemostatic properties. These properties are closely related to wound healing. This study aimed to evaluate the wound healing properties of B. frutescens leaves extract (BFLE) in vitro an...

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Main Authors: Safwan Kamarazaman, Ihsan, Sui Kiong, Ling, Nik Hasan, Mohd Kamal, Basherudin, Norlia, Mohd Kasim, Nur Aini, Ali, Aida Azlina, Ramli, Salfarina, Maniam, Sandra, Johari James, Richard, Rojsitthisak, Pornchai, Halim, Hasseri
Format: Article
Language:English
Published: Elsevier 2024
Online Access:http://psasir.upm.edu.my/id/eprint/113425/1/113425.pdf
http://psasir.upm.edu.my/id/eprint/113425/
https://linkinghub.elsevier.com/retrieve/pii/S1319016424001609
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spelling my.upm.eprints.1134252024-11-25T02:23:09Z http://psasir.upm.edu.my/id/eprint/113425/ Baeckea frutescens L. Promotes wound healing by upregulating expression of TGF-β, IL-1 β, VEGF and MMP-2 Safwan Kamarazaman, Ihsan Sui Kiong, Ling Nik Hasan, Mohd Kamal Basherudin, Norlia Mohd Kasim, Nur Aini Ali, Aida Azlina Ramli, Salfarina Maniam, Sandra Johari James, Richard Rojsitthisak, Pornchai Halim, Hasseri Baeckea frutescens L. has been traditionally used for treating snakebites and is known to possess antifebrile and hemostatic properties. These properties are closely related to wound healing. This study aimed to evaluate the wound healing properties of B. frutescens leaves extract (BFLE) in vitro and in vivo. The in vitro study focused on proliferation, migration, and expression of TGF-β, IL-1β, VEGF, and MMP-2 genes and proteins. The in vivo study included excisional wound healing, histology, and tensile strength studies. The ethanolic extract of B. frutescens (BFLE) was tested for its effects on proliferation and migration using keratinocytes (HaCaT) and fibroblasts (BJ) cells. Gene and protein expression related to wound healing were analyzed using real-time PCR and Western blot assays. The wound healing properties of BFLE were evaluated in vivo using Wistar albino rats, focusing on excisional wound healing, histology, and tensile strength studies. The BFLE displayed significant proliferative and migratory effects on keratinocytes and fibroblasts cells, while upregulating the expression of TGF-β, IL-1β, VEGF, and MMP-2 genes and proteins. BFLE also exhibited significant wound healing effects on Wistar albino rats’ excisional wounds and improved the overall tensile strength. The results suggest that BFLE has strong wound healing properties, as demonstrated by its ability to increase keratinocytes and fibroblasts proliferation and migration, upregulate genes and proteins involved in the wound healing process, and improve wound healing rates and tensile strength. The findings of this study provide important insights into the potential use of B. frutescens as a natural wound healing agent. Elsevier 2024 Article PeerReviewed text en cc_by_nc_nd_4 http://psasir.upm.edu.my/id/eprint/113425/1/113425.pdf Safwan Kamarazaman, Ihsan and Sui Kiong, Ling and Nik Hasan, Mohd Kamal and Basherudin, Norlia and Mohd Kasim, Nur Aini and Ali, Aida Azlina and Ramli, Salfarina and Maniam, Sandra and Johari James, Richard and Rojsitthisak, Pornchai and Halim, Hasseri (2024) Baeckea frutescens L. Promotes wound healing by upregulating expression of TGF-β, IL-1 β, VEGF and MMP-2. Saudi Pharmaceutical Journal, 32 (7). art. no. 102110. pp. 1-13. ISSN 1319-0164; eISSN: 1319-0164 https://linkinghub.elsevier.com/retrieve/pii/S1319016424001609 10.1016/j.jsps.2024.102110
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Baeckea frutescens L. has been traditionally used for treating snakebites and is known to possess antifebrile and hemostatic properties. These properties are closely related to wound healing. This study aimed to evaluate the wound healing properties of B. frutescens leaves extract (BFLE) in vitro and in vivo. The in vitro study focused on proliferation, migration, and expression of TGF-β, IL-1β, VEGF, and MMP-2 genes and proteins. The in vivo study included excisional wound healing, histology, and tensile strength studies. The ethanolic extract of B. frutescens (BFLE) was tested for its effects on proliferation and migration using keratinocytes (HaCaT) and fibroblasts (BJ) cells. Gene and protein expression related to wound healing were analyzed using real-time PCR and Western blot assays. The wound healing properties of BFLE were evaluated in vivo using Wistar albino rats, focusing on excisional wound healing, histology, and tensile strength studies. The BFLE displayed significant proliferative and migratory effects on keratinocytes and fibroblasts cells, while upregulating the expression of TGF-β, IL-1β, VEGF, and MMP-2 genes and proteins. BFLE also exhibited significant wound healing effects on Wistar albino rats’ excisional wounds and improved the overall tensile strength. The results suggest that BFLE has strong wound healing properties, as demonstrated by its ability to increase keratinocytes and fibroblasts proliferation and migration, upregulate genes and proteins involved in the wound healing process, and improve wound healing rates and tensile strength. The findings of this study provide important insights into the potential use of B. frutescens as a natural wound healing agent.
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author Safwan Kamarazaman, Ihsan
Sui Kiong, Ling
Nik Hasan, Mohd Kamal
Basherudin, Norlia
Mohd Kasim, Nur Aini
Ali, Aida Azlina
Ramli, Salfarina
Maniam, Sandra
Johari James, Richard
Rojsitthisak, Pornchai
Halim, Hasseri
spellingShingle Safwan Kamarazaman, Ihsan
Sui Kiong, Ling
Nik Hasan, Mohd Kamal
Basherudin, Norlia
Mohd Kasim, Nur Aini
Ali, Aida Azlina
Ramli, Salfarina
Maniam, Sandra
Johari James, Richard
Rojsitthisak, Pornchai
Halim, Hasseri
Baeckea frutescens L. Promotes wound healing by upregulating expression of TGF-β, IL-1 β, VEGF and MMP-2
author_facet Safwan Kamarazaman, Ihsan
Sui Kiong, Ling
Nik Hasan, Mohd Kamal
Basherudin, Norlia
Mohd Kasim, Nur Aini
Ali, Aida Azlina
Ramli, Salfarina
Maniam, Sandra
Johari James, Richard
Rojsitthisak, Pornchai
Halim, Hasseri
author_sort Safwan Kamarazaman, Ihsan
title Baeckea frutescens L. Promotes wound healing by upregulating expression of TGF-β, IL-1 β, VEGF and MMP-2
title_short Baeckea frutescens L. Promotes wound healing by upregulating expression of TGF-β, IL-1 β, VEGF and MMP-2
title_full Baeckea frutescens L. Promotes wound healing by upregulating expression of TGF-β, IL-1 β, VEGF and MMP-2
title_fullStr Baeckea frutescens L. Promotes wound healing by upregulating expression of TGF-β, IL-1 β, VEGF and MMP-2
title_full_unstemmed Baeckea frutescens L. Promotes wound healing by upregulating expression of TGF-β, IL-1 β, VEGF and MMP-2
title_sort baeckea frutescens l. promotes wound healing by upregulating expression of tgf-β, il-1 β, vegf and mmp-2
publisher Elsevier
publishDate 2024
url http://psasir.upm.edu.my/id/eprint/113425/1/113425.pdf
http://psasir.upm.edu.my/id/eprint/113425/
https://linkinghub.elsevier.com/retrieve/pii/S1319016424001609
_version_ 1817844635892973568
score 13.223943

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