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Understanding the environmental and herbicide response of Lasiodiplodia theobromae and Bipolaris bicolor isolated from infected Eleusine indica

Background: In a prior study, Lasiodiplodia theobromae (Pat.) Griffiths and Maubl. and Bipolaris bicolor (Mitra) Shoemaker., were found to suppress the growth of Eleusine indica (L.) Gaertn, but limited information exists on their response to environmental factors and herbicides for integrated E. in...

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Main Authors: Fakri, Muhammad Aiman, Sapak, Zaiton, Ahmad Hamdani, Muhammad Saiful, Seng, Chuah Tse
Format: Article
Language:English
Published: Sociedade Brasileira da Ciencia das Plantas Daninha 2024
Online Access:http://psasir.upm.edu.my/id/eprint/113416/1/113416.pdf
http://psasir.upm.edu.my/id/eprint/113416/
https://awsjournal.org/article/understanding-the-environmental-and-herbicide-response-of-lasiodiplodia-theobromae-and-bipolaris-bicolor-isolated-from-infected-eleusine-indica/
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spelling my.upm.eprints.1134162024-11-22T08:40:47Z http://psasir.upm.edu.my/id/eprint/113416/ Understanding the environmental and herbicide response of Lasiodiplodia theobromae and Bipolaris bicolor isolated from infected Eleusine indica Fakri, Muhammad Aiman Sapak, Zaiton Ahmad Hamdani, Muhammad Saiful Seng, Chuah Tse Background: In a prior study, Lasiodiplodia theobromae (Pat.) Griffiths and Maubl. and Bipolaris bicolor (Mitra) Shoemaker., were found to suppress the growth of Eleusine indica (L.) Gaertn, but limited information exists on their response to environmental factors and herbicides for integrated E. indica control. Objective: This study aimed to determine the tolerance levels of L. theobromae and B. bicolor to pH, temperature, photoperiod, relative humidity, and herbicides. Methods: The mycelia and conidia of L. theobromae and B. bicolor were exposed to a range of environmental conditions and herbicides in a controlled setting, including different levels of temperature (25, 30, 35 ⁰C), pH (4, 6, 8, 10), photoperiod (24 hours of darkness; alternating 12 hours of blue light followed by 12 hours of darkness; and alternating cycles of 6 hours of blue light and 6 hours of darkness), and relative humidity (75, 85, 95%). Herbicides such as topramezone, diuron, oxyfluorfen, and imazethapyr were applied at their recommended rates. Conidial germination was assessed by counting after an 18-hour incubation using a haemocytometer, while the diameter of mycelium growth was measured after 3 days of incubation, except for the herbicide effects evaluation, which were extended over 7 days. Results: Laboratory assays demonstrated that L. theobromae exhibited higher conidial germination of 85-95% and superior mycelial growth under varied pH, temperature, and photoperiod conditions compared to B. bicolor. Lasiodioplodia theobromae’s mycelia remained unaffected by herbicides at full labelled rates, but they inhibited the conidial germination of the fungus. For B. bicolor, the conidia were not affected by imazethapyr and topramezone, but its mycelial growth was reduced by imazethapyr, oxyfluorfen, and diuron. Conclusions: These results indicate that the mycelia of L. theobromae presents a more favorable option for tank mixing with test herbicides, offering potential for the formulation of an integrated control strategy against E. indica. Sociedade Brasileira da Ciencia das Plantas Daninha 2024 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/113416/1/113416.pdf Fakri, Muhammad Aiman and Sapak, Zaiton and Ahmad Hamdani, Muhammad Saiful and Seng, Chuah Tse (2024) Understanding the environmental and herbicide response of Lasiodiplodia theobromae and Bipolaris bicolor isolated from infected Eleusine indica. Advances in Weed Science, 42. art. no. e020240063. pp. 1-9. ISSN 2675-9462; eISSN: 2675-9462 https://awsjournal.org/article/understanding-the-environmental-and-herbicide-response-of-lasiodiplodia-theobromae-and-bipolaris-bicolor-isolated-from-infected-eleusine-indica/ 10.51694/AdvWeedSci/2024;42:00005
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Background: In a prior study, Lasiodiplodia theobromae (Pat.) Griffiths and Maubl. and Bipolaris bicolor (Mitra) Shoemaker., were found to suppress the growth of Eleusine indica (L.) Gaertn, but limited information exists on their response to environmental factors and herbicides for integrated E. indica control. Objective: This study aimed to determine the tolerance levels of L. theobromae and B. bicolor to pH, temperature, photoperiod, relative humidity, and herbicides. Methods: The mycelia and conidia of L. theobromae and B. bicolor were exposed to a range of environmental conditions and herbicides in a controlled setting, including different levels of temperature (25, 30, 35 ⁰C), pH (4, 6, 8, 10), photoperiod (24 hours of darkness; alternating 12 hours of blue light followed by 12 hours of darkness; and alternating cycles of 6 hours of blue light and 6 hours of darkness), and relative humidity (75, 85, 95%). Herbicides such as topramezone, diuron, oxyfluorfen, and imazethapyr were applied at their recommended rates. Conidial germination was assessed by counting after an 18-hour incubation using a haemocytometer, while the diameter of mycelium growth was measured after 3 days of incubation, except for the herbicide effects evaluation, which were extended over 7 days. Results: Laboratory assays demonstrated that L. theobromae exhibited higher conidial germination of 85-95% and superior mycelial growth under varied pH, temperature, and photoperiod conditions compared to B. bicolor. Lasiodioplodia theobromae’s mycelia remained unaffected by herbicides at full labelled rates, but they inhibited the conidial germination of the fungus. For B. bicolor, the conidia were not affected by imazethapyr and topramezone, but its mycelial growth was reduced by imazethapyr, oxyfluorfen, and diuron. Conclusions: These results indicate that the mycelia of L. theobromae presents a more favorable option for tank mixing with test herbicides, offering potential for the formulation of an integrated control strategy against E. indica.
format Article
author Fakri, Muhammad Aiman
Sapak, Zaiton
Ahmad Hamdani, Muhammad Saiful
Seng, Chuah Tse
spellingShingle Fakri, Muhammad Aiman
Sapak, Zaiton
Ahmad Hamdani, Muhammad Saiful
Seng, Chuah Tse
Understanding the environmental and herbicide response of Lasiodiplodia theobromae and Bipolaris bicolor isolated from infected Eleusine indica
author_facet Fakri, Muhammad Aiman
Sapak, Zaiton
Ahmad Hamdani, Muhammad Saiful
Seng, Chuah Tse
author_sort Fakri, Muhammad Aiman
title Understanding the environmental and herbicide response of Lasiodiplodia theobromae and Bipolaris bicolor isolated from infected Eleusine indica
title_short Understanding the environmental and herbicide response of Lasiodiplodia theobromae and Bipolaris bicolor isolated from infected Eleusine indica
title_full Understanding the environmental and herbicide response of Lasiodiplodia theobromae and Bipolaris bicolor isolated from infected Eleusine indica
title_fullStr Understanding the environmental and herbicide response of Lasiodiplodia theobromae and Bipolaris bicolor isolated from infected Eleusine indica
title_full_unstemmed Understanding the environmental and herbicide response of Lasiodiplodia theobromae and Bipolaris bicolor isolated from infected Eleusine indica
title_sort understanding the environmental and herbicide response of lasiodiplodia theobromae and bipolaris bicolor isolated from infected eleusine indica
publisher Sociedade Brasileira da Ciencia das Plantas Daninha
publishDate 2024
url http://psasir.upm.edu.my/id/eprint/113416/1/113416.pdf
http://psasir.upm.edu.my/id/eprint/113416/
https://awsjournal.org/article/understanding-the-environmental-and-herbicide-response-of-lasiodiplodia-theobromae-and-bipolaris-bicolor-isolated-from-infected-eleusine-indica/
_version_ 1817844634412384256
score 13.223943

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