Staff View: Isolation and physical characterization of hydrophobin-like proteins (HLP) from aerial conidia of metarhizium anisopliae var. anisopliae

Isolation and physical characterization of hydrophobin-like proteins (HLP) from aerial conidia of metarhizium anisopliae var. anisopliae

The aim of this study was to isolate and physically characterized Hyrophobin-Like Proteins (HLP) from the aerial conidia of two local isolates of Metarhizium anisopliae var. anisopliae, namely, TA and LR2. The protein samples were isolated based on their insolubility in hot Sodium Dodecyl Sulfate (S...

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Main Authors:	King, Patricia Jie Hung, Kiong, Diana Siaw Boon, Choon, Fah Josept Bong
Format:	Article
Published:	Science Publications 2015
Subjects:	Metarhizium anisopliae - Research Food - Biotechnology - Malaysia Agricultural - Research
Online Access:	http://psasir.upm.edu.my/id/eprint/113321/ http://www.scopus.com/inward/record.url?eid=2-s2.0-84933524313&partnerID=MN8TOARS
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id	my.upm.eprints.113321
record_format	eprints
spelling	my.upm.eprints.1133212024-11-25T07:07:12Z http://psasir.upm.edu.my/id/eprint/113321/ Isolation and physical characterization of hydrophobin-like proteins (HLP) from aerial conidia of metarhizium anisopliae var. anisopliae King, Patricia Jie Hung Kiong, Diana Siaw Boon Choon, Fah Josept Bong The aim of this study was to isolate and physically characterized Hyrophobin-Like Proteins (HLP) from the aerial conidia of two local isolates of Metarhizium anisopliae var. anisopliae, namely, TA and LR2. The protein samples were isolated based on their insolubility in hot Sodium Dodecyl Sulfate (SDS). The SDS-insoluble proteins were then purified in cold 98% formic acid and performic acid. The molecular weight of the Formic Acid Extracted (FAE) proteins fell in the range of 13-17 kDa with the contents of 3.80 and 3.56 mg mL-1 for TA and LR2, respectively. Due to the stringent protocol of isolation and purification, the FAE proteins can be verified as Hydrophobin-Like Proteins (HLP). Both the HLP samples isolated from M. anisopliae isolates TA and LR2 have low contact angles of 57.06±0.38 and 58.43±0.25°, respectively. The HLP also revealed good emulsification effect in the oil-water phase. Conidia showed good dispersion in aqueous solution with the application of HLP. Paper pre-coated with HLP resisted wetting by water for up to 439 sec. These unique properties of HLP from local isolates of M. anisopliae var. anisopliae are of great potential to be used in wide range of industrial applications. Science Publications 2015-01-29 Article PeerReviewed King, Patricia Jie Hung and Kiong, Diana Siaw Boon and Choon, Fah Josept Bong (2015) Isolation and physical characterization of hydrophobin-like proteins (HLP) from aerial conidia of metarhizium anisopliae var. anisopliae. American Journal of Biochemistry and Biotechnology, 11 (2). pp. 66-72. ISSN 1553468 http://www.scopus.com/inward/record.url?eid=2-s2.0-84933524313&partnerID=MN8TOARS Metarhizium anisopliae - Research Food - Biotechnology - Malaysia Agricultural - Research 10.3844/ajbbsp.2015.66.72
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
topic	Metarhizium anisopliae - Research Food - Biotechnology - Malaysia Agricultural - Research
spellingShingle	Metarhizium anisopliae - Research Food - Biotechnology - Malaysia Agricultural - Research King, Patricia Jie Hung Kiong, Diana Siaw Boon Choon, Fah Josept Bong Isolation and physical characterization of hydrophobin-like proteins (HLP) from aerial conidia of metarhizium anisopliae var. anisopliae
description	The aim of this study was to isolate and physically characterized Hyrophobin-Like Proteins (HLP) from the aerial conidia of two local isolates of Metarhizium anisopliae var. anisopliae, namely, TA and LR2. The protein samples were isolated based on their insolubility in hot Sodium Dodecyl Sulfate (SDS). The SDS-insoluble proteins were then purified in cold 98% formic acid and performic acid. The molecular weight of the Formic Acid Extracted (FAE) proteins fell in the range of 13-17 kDa with the contents of 3.80 and 3.56 mg mL-1 for TA and LR2, respectively. Due to the stringent protocol of isolation and purification, the FAE proteins can be verified as Hydrophobin-Like Proteins (HLP). Both the HLP samples isolated from M. anisopliae isolates TA and LR2 have low contact angles of 57.06±0.38 and 58.43±0.25°, respectively. The HLP also revealed good emulsification effect in the oil-water phase. Conidia showed good dispersion in aqueous solution with the application of HLP. Paper pre-coated with HLP resisted wetting by water for up to 439 sec. These unique properties of HLP from local isolates of M. anisopliae var. anisopliae are of great potential to be used in wide range of industrial applications.
format	Article
author	King, Patricia Jie Hung Kiong, Diana Siaw Boon Choon, Fah Josept Bong
author_facet	King, Patricia Jie Hung Kiong, Diana Siaw Boon Choon, Fah Josept Bong
author_sort	King, Patricia Jie Hung
title	Isolation and physical characterization of hydrophobin-like proteins (HLP) from aerial conidia of metarhizium anisopliae var. anisopliae
title_short	Isolation and physical characterization of hydrophobin-like proteins (HLP) from aerial conidia of metarhizium anisopliae var. anisopliae
title_full	Isolation and physical characterization of hydrophobin-like proteins (HLP) from aerial conidia of metarhizium anisopliae var. anisopliae
title_fullStr	Isolation and physical characterization of hydrophobin-like proteins (HLP) from aerial conidia of metarhizium anisopliae var. anisopliae
title_full_unstemmed	Isolation and physical characterization of hydrophobin-like proteins (HLP) from aerial conidia of metarhizium anisopliae var. anisopliae
title_sort	isolation and physical characterization of hydrophobin-like proteins (hlp) from aerial conidia of metarhizium anisopliae var. anisopliae
publisher	Science Publications
publishDate	2015
url	http://psasir.upm.edu.my/id/eprint/113321/ http://www.scopus.com/inward/record.url?eid=2-s2.0-84933524313&partnerID=MN8TOARS
_version_	1817844625070620672
score	13.222552

Isolation and physical characterization of hydrophobin-like proteins (HLP) from aerial conidia of metarhizium anisopliae var. anisopliae

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