Efficacy of intranasal administration of formalin-killed Pasteurella haemolytica A2 against intratracheal challenge in goats
A trial was conducted to compare the efficacy of intranasal vaccination in protecting goats against pneumonic pasteurellosis with intramuscular vaccination using an oil adjuvant vaccine, and a combination of the two methods. Forty goats were divided into four equal groups. Group 1 was vaccinated twi...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Published: |
British Veterinary Associationadminoff@bsava.com
1998
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/113141/ https://bvajournals.onlinelibrary.wiley.com/doi/10.1136/vr.142.16.428 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
my.upm.eprints.113141 |
|---|---|
| record_format |
eprints |
| spelling |
my.upm.eprints.1131412025-02-05T06:53:26Z http://psasir.upm.edu.my/id/eprint/113141/ Efficacy of intranasal administration of formalin-killed Pasteurella haemolytica A2 against intratracheal challenge in goats Effendy, A.W.M. Zamri-Saad, M. Puspa, R. Rosiah, S. A trial was conducted to compare the efficacy of intranasal vaccination in protecting goats against pneumonic pasteurellosis with intramuscular vaccination using an oil adjuvant vaccine, and a combination of the two methods. Forty goats were divided into four equal groups. Group 1 was vaccinated twice intranasally with formalin-killed Pasteurella haemolytica A2, group 2 was vaccinated twice intramuscularly with an oil adjuvant vaccine containing P haemolytica A7, and group 3 was initially vaccinated intranasally with the formalin-killed P haemolytica A2 followed by intramuscular vaccination with the oil adjuvant vaccine. In each group the two vaccinations were carried out four weeks apart. Group 4 was the unvaccinated control group. All goats were challenged intratracheally with 4 ml of an inoculum containing live P haemolytica A2 at a concentration of 1.3 x 107 colony forming units/ml two weeks after the last vaccination and were killed 14 days after the challenge. Although group 2 showed the highest clinical score following the challenge, deaths were observed only in group 3. Three goats in group 1 had pneumonic lung lesions, compared with six goats in group 2 and all the goats in groups 3 and 4. The lung lesions in group 1 were significantly (P < 0.05) less severe than in groups 3 and 4. Similarly, the lesions in group 2 were markedly less severe than in groups 3 and 4, although the differences were not significant. The difference between the extent of the lung lesions in the goats in groups 1 and 2 was not significant. Antibody against P haemolytica A2 in group 1 reached peak levels and was significantly (P < 0.01) higher than in the control group one week after the second vaccination, before declining. British Veterinary Associationadminoff@bsava.com 1998 Article PeerReviewed Effendy, A.W.M. and Zamri-Saad, M. and Puspa, R. and Rosiah, S. (1998) Efficacy of intranasal administration of formalin-killed Pasteurella haemolytica A2 against intratracheal challenge in goats. Veterinary Record, 142 (16). pp. 428-431. ISSN 0042-4900; eISSN: 0042-4900 https://bvajournals.onlinelibrary.wiley.com/doi/10.1136/vr.142.16.428 10.1136/vr.142.16.428 |
| institution |
Universiti Putra Malaysia |
| building |
UPM Library |
| collection |
Institutional Repository |
| continent |
Asia |
| country |
Malaysia |
| content_provider |
Universiti Putra Malaysia |
| content_source |
UPM Institutional Repository |
| url_provider |
http://psasir.upm.edu.my/ |
| description |
A trial was conducted to compare the efficacy of intranasal vaccination in protecting goats against pneumonic pasteurellosis with intramuscular vaccination using an oil adjuvant vaccine, and a combination of the two methods. Forty goats were divided into four equal groups. Group 1 was vaccinated twice intranasally with formalin-killed Pasteurella haemolytica A2, group 2 was vaccinated twice intramuscularly with an oil adjuvant vaccine containing P haemolytica A7, and group 3 was initially vaccinated intranasally with the formalin-killed P haemolytica A2 followed by intramuscular vaccination with the oil adjuvant vaccine. In each group the two vaccinations were carried out four weeks apart. Group 4 was the unvaccinated control group. All goats were challenged intratracheally with 4 ml of an inoculum containing live P haemolytica A2 at a concentration of 1.3 x 107 colony forming units/ml two weeks after the last vaccination and were killed 14 days after the challenge. Although group 2 showed the highest clinical score following the challenge, deaths were observed only in group 3. Three goats in group 1 had pneumonic lung lesions, compared with six goats in group 2 and all the goats in groups 3 and 4. The lung lesions in group 1 were significantly (P < 0.05) less severe than in groups 3 and 4. Similarly, the lesions in group 2 were markedly less severe than in groups 3 and 4, although the differences were not significant. The difference between the extent of the lung lesions in the goats in groups 1 and 2 was not significant. Antibody against P haemolytica A2 in group 1 reached peak levels and was significantly (P < 0.01) higher than in the control group one week after the second vaccination, before declining. |
| format |
Article |
| author |
Effendy, A.W.M. Zamri-Saad, M. Puspa, R. Rosiah, S. |
| spellingShingle |
Effendy, A.W.M. Zamri-Saad, M. Puspa, R. Rosiah, S. Efficacy of intranasal administration of formalin-killed Pasteurella haemolytica A2 against intratracheal challenge in goats |
| author_facet |
Effendy, A.W.M. Zamri-Saad, M. Puspa, R. Rosiah, S. |
| author_sort |
Effendy, A.W.M. |
| title |
Efficacy of intranasal administration of formalin-killed Pasteurella haemolytica A2 against intratracheal challenge in goats |
| title_short |
Efficacy of intranasal administration of formalin-killed Pasteurella haemolytica A2 against intratracheal challenge in goats |
| title_full |
Efficacy of intranasal administration of formalin-killed Pasteurella haemolytica A2 against intratracheal challenge in goats |
| title_fullStr |
Efficacy of intranasal administration of formalin-killed Pasteurella haemolytica A2 against intratracheal challenge in goats |
| title_full_unstemmed |
Efficacy of intranasal administration of formalin-killed Pasteurella haemolytica A2 against intratracheal challenge in goats |
| title_sort |
efficacy of intranasal administration of formalin-killed pasteurella haemolytica a2 against intratracheal challenge in goats |
| publisher |
British Veterinary Associationadminoff@bsava.com |
| publishDate |
1998 |
| url |
http://psasir.upm.edu.my/id/eprint/113141/ https://bvajournals.onlinelibrary.wiley.com/doi/10.1136/vr.142.16.428 |
| _version_ |
1823276349994827776 |
| score |
13.244413 |