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Effects of supplementing coated methionine in a high plant-protein diet on growth, antioxidant capacity, digestive enzymes activity and expression of TOR signaling pathway associated genes in gibel carp, Carassius auratus gibelio

This study explored the impacts of supplementation of different levels of coated methionine (Met) in a high-plant protein diet on growth, blood biochemistry, antioxidant capacity, digestive enzymes activity and expression of genes related to TOR signaling pathway in gibel carp (Carassius auratus gib...

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Main Authors: Du, Yingying, Lin, Xiaowen, Shao, Xianping, Zhao, Jianhua, Xu, Hong, de Cruz, Clement R., Xu, Qiyou
Format: Article
Language:English
Published: Frontiers Media 2024
Online Access:http://psasir.upm.edu.my/id/eprint/112912/1/112912.pdf
http://psasir.upm.edu.my/id/eprint/112912/
https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2024.1319698/full
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spelling my.upm.eprints.1129122024-10-28T07:40:45Z http://psasir.upm.edu.my/id/eprint/112912/ Effects of supplementing coated methionine in a high plant-protein diet on growth, antioxidant capacity, digestive enzymes activity and expression of TOR signaling pathway associated genes in gibel carp, Carassius auratus gibelio Du, Yingying Lin, Xiaowen Shao, Xianping Zhao, Jianhua Xu, Hong de Cruz, Clement R. Xu, Qiyou This study explored the impacts of supplementation of different levels of coated methionine (Met) in a high-plant protein diet on growth, blood biochemistry, antioxidant capacity, digestive enzymes activity and expression of genes related to TOR signaling pathway in gibel carp (Carassius auratus gibeilo). A high-plant protein diet was formulated and used as a basal diet and supplemented with five different levels of coated Met at 0.15, 0.30, 0.45, 0.60 and 0.75%, corresponding to final analyzed Met levels of 0.34, 0.49, 0.64, 0.76, 0.92 and 1.06%. Three replicate groups of fish (initial mean weight, 11.37 ± 0.02 g) (20 fish per replicate) were fed the test diets over a 10-week feeding period. The results indicated that with the increase of coated Met level, the final weight, weight gain (WG) and specific growth rate initially boosted and then suppressed, peaking at 0.76% Met level (P< 0.05). Increasing dietary Met level led to significantly increased muscle crude protein content (P< 0.05) and reduced serum alanine aminotransferase activity (P< 0.05). Using appropriate dietary Met level led to reduced malondialdehyde concentration in hepatopancreas (P< 0.05), improved superoxide dismutase activity (P< 0.05), and enhanced intestinal amylase and protease activities (P< 0.05). The expression levels of genes associated with muscle protein synthesis such as insulin-like growth factor-1, protein kinase B, target of rapamycin and eukaryotic initiation factor 4E binding protein-1 mRNA were significantly regulated, peaking at Met level of 0.76% (P< 0.05). In conclusion, supplementing optimal level of coated Met improved on fish growth, antioxidant capacity, and the expression of TOR pathway related genes in muscle. The optimal dietary Met level was determined to be 0.71% of the diet based on quadratic regression analysis of WG. Copyright © 2024 Du, Lin, Shao, Zhao, Xu, de Cruz and Xu. Frontiers Media 2024 Article PeerReviewed text en cc_by_4 http://psasir.upm.edu.my/id/eprint/112912/1/112912.pdf Du, Yingying and Lin, Xiaowen and Shao, Xianping and Zhao, Jianhua and Xu, Hong and de Cruz, Clement R. and Xu, Qiyou (2024) Effects of supplementing coated methionine in a high plant-protein diet on growth, antioxidant capacity, digestive enzymes activity and expression of TOR signaling pathway associated genes in gibel carp, Carassius auratus gibelio. Frontiers in Immunology, 15. art. no. 1319698. ISSN 1664-3224 https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2024.1319698/full 10.3389/fimmu.2024.1319698
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description This study explored the impacts of supplementation of different levels of coated methionine (Met) in a high-plant protein diet on growth, blood biochemistry, antioxidant capacity, digestive enzymes activity and expression of genes related to TOR signaling pathway in gibel carp (Carassius auratus gibeilo). A high-plant protein diet was formulated and used as a basal diet and supplemented with five different levels of coated Met at 0.15, 0.30, 0.45, 0.60 and 0.75%, corresponding to final analyzed Met levels of 0.34, 0.49, 0.64, 0.76, 0.92 and 1.06%. Three replicate groups of fish (initial mean weight, 11.37 ± 0.02 g) (20 fish per replicate) were fed the test diets over a 10-week feeding period. The results indicated that with the increase of coated Met level, the final weight, weight gain (WG) and specific growth rate initially boosted and then suppressed, peaking at 0.76% Met level (P< 0.05). Increasing dietary Met level led to significantly increased muscle crude protein content (P< 0.05) and reduced serum alanine aminotransferase activity (P< 0.05). Using appropriate dietary Met level led to reduced malondialdehyde concentration in hepatopancreas (P< 0.05), improved superoxide dismutase activity (P< 0.05), and enhanced intestinal amylase and protease activities (P< 0.05). The expression levels of genes associated with muscle protein synthesis such as insulin-like growth factor-1, protein kinase B, target of rapamycin and eukaryotic initiation factor 4E binding protein-1 mRNA were significantly regulated, peaking at Met level of 0.76% (P< 0.05). In conclusion, supplementing optimal level of coated Met improved on fish growth, antioxidant capacity, and the expression of TOR pathway related genes in muscle. The optimal dietary Met level was determined to be 0.71% of the diet based on quadratic regression analysis of WG. Copyright © 2024 Du, Lin, Shao, Zhao, Xu, de Cruz and Xu.
format Article
author Du, Yingying
Lin, Xiaowen
Shao, Xianping
Zhao, Jianhua
Xu, Hong
de Cruz, Clement R.
Xu, Qiyou
spellingShingle Du, Yingying
Lin, Xiaowen
Shao, Xianping
Zhao, Jianhua
Xu, Hong
de Cruz, Clement R.
Xu, Qiyou
Effects of supplementing coated methionine in a high plant-protein diet on growth, antioxidant capacity, digestive enzymes activity and expression of TOR signaling pathway associated genes in gibel carp, Carassius auratus gibelio
author_facet Du, Yingying
Lin, Xiaowen
Shao, Xianping
Zhao, Jianhua
Xu, Hong
de Cruz, Clement R.
Xu, Qiyou
author_sort Du, Yingying
title Effects of supplementing coated methionine in a high plant-protein diet on growth, antioxidant capacity, digestive enzymes activity and expression of TOR signaling pathway associated genes in gibel carp, Carassius auratus gibelio
title_short Effects of supplementing coated methionine in a high plant-protein diet on growth, antioxidant capacity, digestive enzymes activity and expression of TOR signaling pathway associated genes in gibel carp, Carassius auratus gibelio
title_full Effects of supplementing coated methionine in a high plant-protein diet on growth, antioxidant capacity, digestive enzymes activity and expression of TOR signaling pathway associated genes in gibel carp, Carassius auratus gibelio
title_fullStr Effects of supplementing coated methionine in a high plant-protein diet on growth, antioxidant capacity, digestive enzymes activity and expression of TOR signaling pathway associated genes in gibel carp, Carassius auratus gibelio
title_full_unstemmed Effects of supplementing coated methionine in a high plant-protein diet on growth, antioxidant capacity, digestive enzymes activity and expression of TOR signaling pathway associated genes in gibel carp, Carassius auratus gibelio
title_sort effects of supplementing coated methionine in a high plant-protein diet on growth, antioxidant capacity, digestive enzymes activity and expression of tor signaling pathway associated genes in gibel carp, carassius auratus gibelio
publisher Frontiers Media
publishDate 2024
url http://psasir.upm.edu.my/id/eprint/112912/1/112912.pdf
http://psasir.upm.edu.my/id/eprint/112912/
https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2024.1319698/full
_version_ 1814936535131226112
score 13.211869

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