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Gene Expression And Metabonomic Profilings In P53+/- Knockout Mice Following Diethylstilbestrol Treatment

Clastogenic carcinogen diethylstilbestrol (DES) results in a broad of spectrum of toxic and carcinogenic effects in humans and rodents. Female C57BL/6J wild-type mice (23-25g) and female p53 +/1- hemizygous mice were treated with DES (500 umol/kg) i .p., once daily for 4 days. Control animals wer...

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Main Author: Salleh, Mohd Nazil
Format: Thesis
Language:English
English
Published: 2003
Online Access:http://psasir.upm.edu.my/id/eprint/11274/1/FPSK_P_2003_2.pdf
http://psasir.upm.edu.my/id/eprint/11274/
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spelling my.upm.eprints.112742024-06-13T07:34:40Z http://psasir.upm.edu.my/id/eprint/11274/ Gene Expression And Metabonomic Profilings In P53+/- Knockout Mice Following Diethylstilbestrol Treatment Salleh, Mohd Nazil Clastogenic carcinogen diethylstilbestrol (DES) results in a broad of spectrum of toxic and carcinogenic effects in humans and rodents. Female C57BL/6J wild-type mice (23-25g) and female p53 +/1- hemizygous mice were treated with DES (500 umol/kg) i .p., once daily for 4 days. Control animals were treated with the trioctanoin vehicle only. All animals were sacrificed 24 hours after the last dose, and liver, kidney and uterus were harvested and frozen at -80oC . Analysis of differential expression levels of multiple genes involved in apoptosis and cell was performed using cDNA array technology. Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) analysis was carried out in order to verify the expression of these genes. In hepatocytes, murine apoptosis arrays showed three genes: bcl-w, caspase-3 and E2Fl were transcriptionally down-regulated and eight genes: bad, bax, caspase-1, caspase-7, c-myc, p2I, p53 and Rb were up-regulated. In cell-cycle arrays thirteen genes were up-regulated: CDK6, CKK1, Cyclin C, Cyclin D2, Cyclin D3, Cyclin E, Cyclin E2, E2F1, pI6, p19, p21, p57 and Skp1. The greatest change was inp21 gene expression. There was a 3 -fold versus 10-fold increase induction for apoptosis- 2003-05 Thesis NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/11274/1/FPSK_P_2003_2.pdf Salleh, Mohd Nazil (2003) Gene Expression And Metabonomic Profilings In P53+/- Knockout Mice Following Diethylstilbestrol Treatment. Doctoral thesis, Universiti Putra Malaysia. English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description Clastogenic carcinogen diethylstilbestrol (DES) results in a broad of spectrum of toxic and carcinogenic effects in humans and rodents. Female C57BL/6J wild-type mice (23-25g) and female p53 +/1- hemizygous mice were treated with DES (500 umol/kg) i .p., once daily for 4 days. Control animals were treated with the trioctanoin vehicle only. All animals were sacrificed 24 hours after the last dose, and liver, kidney and uterus were harvested and frozen at -80oC . Analysis of differential expression levels of multiple genes involved in apoptosis and cell was performed using cDNA array technology. Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) analysis was carried out in order to verify the expression of these genes. In hepatocytes, murine apoptosis arrays showed three genes: bcl-w, caspase-3 and E2Fl were transcriptionally down-regulated and eight genes: bad, bax, caspase-1, caspase-7, c-myc, p2I, p53 and Rb were up-regulated. In cell-cycle arrays thirteen genes were up-regulated: CDK6, CKK1, Cyclin C, Cyclin D2, Cyclin D3, Cyclin E, Cyclin E2, E2F1, pI6, p19, p21, p57 and Skp1. The greatest change was inp21 gene expression. There was a 3 -fold versus 10-fold increase induction for apoptosis-
format Thesis
author Salleh, Mohd Nazil
spellingShingle Salleh, Mohd Nazil
Gene Expression And Metabonomic Profilings In P53+/- Knockout Mice Following Diethylstilbestrol Treatment
author_facet Salleh, Mohd Nazil
author_sort Salleh, Mohd Nazil
title Gene Expression And Metabonomic Profilings In P53+/- Knockout Mice Following Diethylstilbestrol Treatment
title_short Gene Expression And Metabonomic Profilings In P53+/- Knockout Mice Following Diethylstilbestrol Treatment
title_full Gene Expression And Metabonomic Profilings In P53+/- Knockout Mice Following Diethylstilbestrol Treatment
title_fullStr Gene Expression And Metabonomic Profilings In P53+/- Knockout Mice Following Diethylstilbestrol Treatment
title_full_unstemmed Gene Expression And Metabonomic Profilings In P53+/- Knockout Mice Following Diethylstilbestrol Treatment
title_sort gene expression and metabonomic profilings in p53+/- knockout mice following diethylstilbestrol treatment
publishDate 2003
url http://psasir.upm.edu.my/id/eprint/11274/1/FPSK_P_2003_2.pdf
http://psasir.upm.edu.my/id/eprint/11274/
_version_ 1802978776382963712
score 13.211869

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