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Itraconazole and fluconazole-induced toxicity in rat hepatocytes: a comparativein vitro study

This current study was to investigate the in vitrocytotoxicity of rat hepatocytes induced by the antifungal drugs, itraconazole and fluconazole. Both antifungal drugs caused dose-dependent cytotoxicity. In vitro incubation of hepatocytes with itraconazole revealed significantly higher lactate dehydr...

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Main Authors: Somchit, N, Hassim, SM, Samsudin, SH
Format: Article
Language:English
Published: SAGE Publications 2002
Online Access:http://psasir.upm.edu.my/id/eprint/112712/1/112712.pdf
http://psasir.upm.edu.my/id/eprint/112712/
https://journals.sagepub.com/doi/10.1191/0960327102ht208oa
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spelling my.upm.eprints.1127122025-03-05T03:37:22Z http://psasir.upm.edu.my/id/eprint/112712/ Itraconazole and fluconazole-induced toxicity in rat hepatocytes: a comparativein vitro study Somchit, N Hassim, SM Samsudin, SH This current study was to investigate the in vitrocytotoxicity of rat hepatocytes induced by the antifungal drugs, itraconazole and fluconazole. Both antifungal drugs caused dose-dependent cytotoxicity. In vitro incubation of hepatocytes with itraconazole revealed significantly higher lactate dehydrogenase (LDH) leakage when compared to fluconazole. Phenobarbital pretreated hepatocytes contained significantly higher total cytochrome P450 content than the control hepatocytes. P450 content was reduced approximately 30% for both types of hepatocytes after 6 hours incubation. Interestingly, cytotoxicity of itraconazole was reduced significantly by phenobarbital pretreatment. Phenobarbital did not have any effect on the cytotoxicity induced by fluconazole. These results demonstrate the in vitro toxicity of hepatocytes induced by itraconazole and fluconazole that were expressed in a dose and time-dependent manner. Phenobarbital plays a role in the cytoprotection of hepatocytes to itraconazole-induced but not fluconazole-induced cytotoxicity in vitro. SAGE Publications 2002 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/112712/1/112712.pdf Somchit, N and Hassim, SM and Samsudin, SH (2002) Itraconazole and fluconazole-induced toxicity in rat hepatocytes: a comparativein vitro study. Human & Experimental Toxicology, 21 (1). pp. 43-48. ISSN 0960-3271; eISSN: 1477-0903 https://journals.sagepub.com/doi/10.1191/0960327102ht208oa 10.1191/0960327102ht208oa
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description This current study was to investigate the in vitrocytotoxicity of rat hepatocytes induced by the antifungal drugs, itraconazole and fluconazole. Both antifungal drugs caused dose-dependent cytotoxicity. In vitro incubation of hepatocytes with itraconazole revealed significantly higher lactate dehydrogenase (LDH) leakage when compared to fluconazole. Phenobarbital pretreated hepatocytes contained significantly higher total cytochrome P450 content than the control hepatocytes. P450 content was reduced approximately 30% for both types of hepatocytes after 6 hours incubation. Interestingly, cytotoxicity of itraconazole was reduced significantly by phenobarbital pretreatment. Phenobarbital did not have any effect on the cytotoxicity induced by fluconazole. These results demonstrate the in vitro toxicity of hepatocytes induced by itraconazole and fluconazole that were expressed in a dose and time-dependent manner. Phenobarbital plays a role in the cytoprotection of hepatocytes to itraconazole-induced but not fluconazole-induced cytotoxicity in vitro.
format Article
author Somchit, N
Hassim, SM
Samsudin, SH
spellingShingle Somchit, N
Hassim, SM
Samsudin, SH
Itraconazole and fluconazole-induced toxicity in rat hepatocytes: a comparativein vitro study
author_facet Somchit, N
Hassim, SM
Samsudin, SH
author_sort Somchit, N
title Itraconazole and fluconazole-induced toxicity in rat hepatocytes: a comparativein vitro study
title_short Itraconazole and fluconazole-induced toxicity in rat hepatocytes: a comparativein vitro study
title_full Itraconazole and fluconazole-induced toxicity in rat hepatocytes: a comparativein vitro study
title_fullStr Itraconazole and fluconazole-induced toxicity in rat hepatocytes: a comparativein vitro study
title_full_unstemmed Itraconazole and fluconazole-induced toxicity in rat hepatocytes: a comparativein vitro study
title_sort itraconazole and fluconazole-induced toxicity in rat hepatocytes: a comparativein vitro study
publisher SAGE Publications
publishDate 2002
url http://psasir.upm.edu.my/id/eprint/112712/1/112712.pdf
http://psasir.upm.edu.my/id/eprint/112712/
https://journals.sagepub.com/doi/10.1191/0960327102ht208oa
_version_ 1825810711606984704
score 13.244413

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