Characterisation Of Lactic Acid Bacteria And Bifidobacteria And Their Potential Application As A Probiotic Against Infant Diarrhea
The major health promoting probiotic bacteria found in the human gut are of the genera Bifidobacterium and Lactobacillus. The main objectives of the present studies were to isolate, characterise and identify the suitable probiotic strains of Lactobacillus (LAB) and Bifidobacteria (BB) from faeces...
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| Format: | Thesis |
| Language: | English English |
| Published: |
2000
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| Online Access: | http://psasir.upm.edu.my/id/eprint/11269/1/FPSK_P_2000_2.pdf http://psasir.upm.edu.my/id/eprint/11269/ |
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| Summary: | The major health promoting probiotic bacteria found in the human gut are of
the genera Bifidobacterium and Lactobacillus. The main objectives of the present
studies were to isolate, characterise and identify the suitable probiotic strains of
Lactobacillus (LAB) and Bifidobacteria (BB) from faeces of breast fed infants which
could be used as effective probiotic for the control of infant diarrheal diseases.
From the isolation studies, the Lactobacillus was found to be Gram-positive,
non-motile, short rods and catalase, nitrate, oxidase negative. The Bifidobacteria
were Gram-negative, curved with characteristics of Y and V shapes. The high
performance of liquid chromatography (HPLC) showed that almost all strains of BB
produced more or less or equal amount of acetic and lactic acids. Based on the
carbohydrate fermentation profile using API-CH-50 kits, out of 21 Lactobacillus, 16
species belong to the L. casei, four to L . brevis, one species to L. plantarum and one
sub species casei. Twenty of Bifidobacteria were B. infantis species, where 13
belong to subspecies infantis, 7 were lacentis
The antagonistic activities of LAB and BB were tested against ETEC E.coli
01 57:H7 and Salmonella typhimurium S-285 using double layered assay, results
showed that strains LAB-3, 11, 21 and Bifi- l l , 1 9, 20 produced wider inhibition
zone compared to others. |
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