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Utilisation of enterobacterial repetitive intergenic consensus (ERIC) sequence-based PCR to fingerprint the genomes of Bifidobacterium isolates and other probiotic bacteria

Enterobacterial repetitive intergenic consensus based on PCR (ERIC-PCR) was used to generate DNA fingerprints for bifidobacteria and other probiotic bacteria. Two primers (ERIC 1R and ERIC 2) used in ERIC-PCR revealed that all of the probiotic bacteria tested possess enterobacterial repetitive inter...

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Main Authors: Shuhaimi, M., Ali, A.M., Saleh, N.M., Yazid, A.M.
Format: Article
Language:English
Published: Springer 2001
Online Access:http://psasir.upm.edu.my/id/eprint/112670/1/112670.pdf
http://psasir.upm.edu.my/id/eprint/112670/
https://link.springer.com/article/10.1023/A:1010355003674?error=cookies_not_supported&code=1f499e90-de83-4e55-a364-fcd84f02fa62
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spelling my.upm.eprints.1126702025-03-06T00:58:00Z http://psasir.upm.edu.my/id/eprint/112670/ Utilisation of enterobacterial repetitive intergenic consensus (ERIC) sequence-based PCR to fingerprint the genomes of Bifidobacterium isolates and other probiotic bacteria Shuhaimi, M. Ali, A.M. Saleh, N.M. Yazid, A.M. Enterobacterial repetitive intergenic consensus based on PCR (ERIC-PCR) was used to generate DNA fingerprints for bifidobacteria and other probiotic bacteria. Two primers (ERIC 1R and ERIC 2) used in ERIC-PCR revealed that all of the probiotic bacteria tested possess enterobacterial repetitive intergenic consensus sequences with the PCR products ranging from 250 bp to 5000 bp. The bacterial strains can be differentiated by comparing fingerprint patterns. The dendrogram of the fingerprints revealed that most of the bifidobacterial wild type strains fell into one cluster at similarity level of approximately 79%. Springer 2001 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/112670/1/112670.pdf Shuhaimi, M. and Ali, A.M. and Saleh, N.M. and Yazid, A.M. (2001) Utilisation of enterobacterial repetitive intergenic consensus (ERIC) sequence-based PCR to fingerprint the genomes of Bifidobacterium isolates and other probiotic bacteria. Biotechnology Letters, 23 (9). pp. 731-736. ISSN 0141-5492; eISSN: 1573-6776 https://link.springer.com/article/10.1023/A:1010355003674?error=cookies_not_supported&code=1f499e90-de83-4e55-a364-fcd84f02fa62 10.1023/a:1010355003674
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Enterobacterial repetitive intergenic consensus based on PCR (ERIC-PCR) was used to generate DNA fingerprints for bifidobacteria and other probiotic bacteria. Two primers (ERIC 1R and ERIC 2) used in ERIC-PCR revealed that all of the probiotic bacteria tested possess enterobacterial repetitive intergenic consensus sequences with the PCR products ranging from 250 bp to 5000 bp. The bacterial strains can be differentiated by comparing fingerprint patterns. The dendrogram of the fingerprints revealed that most of the bifidobacterial wild type strains fell into one cluster at similarity level of approximately 79%.
format Article
author Shuhaimi, M.
Ali, A.M.
Saleh, N.M.
Yazid, A.M.
spellingShingle Shuhaimi, M.
Ali, A.M.
Saleh, N.M.
Yazid, A.M.
Utilisation of enterobacterial repetitive intergenic consensus (ERIC) sequence-based PCR to fingerprint the genomes of Bifidobacterium isolates and other probiotic bacteria
author_facet Shuhaimi, M.
Ali, A.M.
Saleh, N.M.
Yazid, A.M.
author_sort Shuhaimi, M.
title Utilisation of enterobacterial repetitive intergenic consensus (ERIC) sequence-based PCR to fingerprint the genomes of Bifidobacterium isolates and other probiotic bacteria
title_short Utilisation of enterobacterial repetitive intergenic consensus (ERIC) sequence-based PCR to fingerprint the genomes of Bifidobacterium isolates and other probiotic bacteria
title_full Utilisation of enterobacterial repetitive intergenic consensus (ERIC) sequence-based PCR to fingerprint the genomes of Bifidobacterium isolates and other probiotic bacteria
title_fullStr Utilisation of enterobacterial repetitive intergenic consensus (ERIC) sequence-based PCR to fingerprint the genomes of Bifidobacterium isolates and other probiotic bacteria
title_full_unstemmed Utilisation of enterobacterial repetitive intergenic consensus (ERIC) sequence-based PCR to fingerprint the genomes of Bifidobacterium isolates and other probiotic bacteria
title_sort utilisation of enterobacterial repetitive intergenic consensus (eric) sequence-based pcr to fingerprint the genomes of bifidobacterium isolates and other probiotic bacteria
publisher Springer
publishDate 2001
url http://psasir.upm.edu.my/id/eprint/112670/1/112670.pdf
http://psasir.upm.edu.my/id/eprint/112670/
https://link.springer.com/article/10.1023/A:1010355003674?error=cookies_not_supported&code=1f499e90-de83-4e55-a364-fcd84f02fa62
_version_ 1825810709964914688
score 13.244413

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