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Molecular And Immunological Characteristics Of Vibrio Cholerae And Vibrio Alginolyticus

Twenty isolates of Vibrio cholerae and four isolates of Vibrio alginolyticus used in this study were identified using the conventional biochemical tests. All of the isolates were screened for the zonula occludens toxin gene (zot) by the Polymerase Chain Reaction (PCR) technique. The 1083 bp fragm...

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Main Author: Neela, Vasanthakumari
Format: Thesis
Language:English
English
Published: 2000
Subjects:
Vibrio cholerae
Vibrionaceae
Gram negative bacteria
Online Access:http://psasir.upm.edu.my/id/eprint/11209/1/FPSK_M_2000_6.pdf
http://psasir.upm.edu.my/id/eprint/11209/
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spelling my.upm.eprints.112092024-06-12T04:38:58Z http://psasir.upm.edu.my/id/eprint/11209/ Molecular And Immunological Characteristics Of Vibrio Cholerae And Vibrio Alginolyticus Neela, Vasanthakumari Twenty isolates of Vibrio cholerae and four isolates of Vibrio alginolyticus used in this study were identified using the conventional biochemical tests. All of the isolates were screened for the zonula occludens toxin gene (zot) by the Polymerase Chain Reaction (PCR) technique. The 1083 bp fragment of the zot gene was successfully amplified in all the tested isolates. Asymmetry PCR was used as a preliminary detection method and later confirmed through the gene probe method to test for the presence of the zot gene. Consequently, the zot gene was successfully cloned in pCR 2.1 TOPO vector and sequenced, in which the sequences were 99% homologous to the gene bank sequences. 2000-05 Thesis NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/11209/1/FPSK_M_2000_6.pdf Neela, Vasanthakumari (2000) Molecular And Immunological Characteristics Of Vibrio Cholerae And Vibrio Alginolyticus. Masters thesis, Universiti Putra Malaysia. Vibrio cholerae Vibrionaceae Gram negative bacteria English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
topic Vibrio cholerae
Vibrionaceae
Gram negative bacteria
spellingShingle Vibrio cholerae
Vibrionaceae
Gram negative bacteria
Neela, Vasanthakumari
Molecular And Immunological Characteristics Of Vibrio Cholerae And Vibrio Alginolyticus
description Twenty isolates of Vibrio cholerae and four isolates of Vibrio alginolyticus used in this study were identified using the conventional biochemical tests. All of the isolates were screened for the zonula occludens toxin gene (zot) by the Polymerase Chain Reaction (PCR) technique. The 1083 bp fragment of the zot gene was successfully amplified in all the tested isolates. Asymmetry PCR was used as a preliminary detection method and later confirmed through the gene probe method to test for the presence of the zot gene. Consequently, the zot gene was successfully cloned in pCR 2.1 TOPO vector and sequenced, in which the sequences were 99% homologous to the gene bank sequences.
format Thesis
author Neela, Vasanthakumari
author_facet Neela, Vasanthakumari
author_sort Neela, Vasanthakumari
title Molecular And Immunological Characteristics Of Vibrio Cholerae And Vibrio Alginolyticus
title_short Molecular And Immunological Characteristics Of Vibrio Cholerae And Vibrio Alginolyticus
title_full Molecular And Immunological Characteristics Of Vibrio Cholerae And Vibrio Alginolyticus
title_fullStr Molecular And Immunological Characteristics Of Vibrio Cholerae And Vibrio Alginolyticus
title_full_unstemmed Molecular And Immunological Characteristics Of Vibrio Cholerae And Vibrio Alginolyticus
title_sort molecular and immunological characteristics of vibrio cholerae and vibrio alginolyticus
publishDate 2000
url http://psasir.upm.edu.my/id/eprint/11209/1/FPSK_M_2000_6.pdf
http://psasir.upm.edu.my/id/eprint/11209/
_version_ 1802978765611991040
score 13.222552

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