Cover Image

Novel peptides that inhibit the propagation of Newcastle disease virus

A disulfide constrained random heptapeptide library displayed on filamentous bacteriophage M13 was applied to select specific ligands that interact with Newcastle disease virus (NDV). A fusion phage carrying the amino acid sequence TLTTKLY was selected from the panning procedure. An antibody competi...

Full description

Saved in:
Bibliographic Details
Main Authors: Ramanujam, P., Tan, W. S., Nathan, S., Yusoff, K.
Format: Article
Published: Springer 2002
Online Access:http://psasir.upm.edu.my/id/eprint/111867/
https://link.springer.com/article/10.1007/s00705-001-0778-y?error=cookies_not_supported&code=4d674463-e51e-4a15-a6d0-a442fc159735
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.111867
record_format eprints
spelling my.upm.eprints.1118672025-03-03T06:21:50Z http://psasir.upm.edu.my/id/eprint/111867/ Novel peptides that inhibit the propagation of Newcastle disease virus Ramanujam, P. Tan, W. S. Nathan, S. Yusoff, K. A disulfide constrained random heptapeptide library displayed on filamentous bacteriophage M13 was applied to select specific ligands that interact with Newcastle disease virus (NDV). A fusion phage carrying the amino acid sequence TLTTKLY was selected from the panning procedure. An antibody competition assay showed that the selected phage was capable of competing with the polyclonal antibodies raised against NDV for binding sites on the virus. Determination of the binding affinity of this phage with NDV by an equilibrium binding assay in solution revealed two different dissociation constants, suggesting that there could be two distinct binding sites for the phage on NDV. Synthetic peptides with the sequence CTLTTKLYC, either in linear or cyclic conformations inhibited the binding of phage bearing the same sequence to NDV. These peptides also inhibited the hemolytic activity of the virus as well as its propagation in embryonated chicken eggs. Springer 2002 Article PeerReviewed Ramanujam, P. and Tan, W. S. and Nathan, S. and Yusoff, K. (2002) Novel peptides that inhibit the propagation of Newcastle disease virus. Archives of Virology, 147 (5). pp. 981-993. ISSN 0304-8608; eISSN: 1432-8798 https://link.springer.com/article/10.1007/s00705-001-0778-y?error=cookies_not_supported&code=4d674463-e51e-4a15-a6d0-a442fc159735 10.1007/s00705-001-0778-y
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description A disulfide constrained random heptapeptide library displayed on filamentous bacteriophage M13 was applied to select specific ligands that interact with Newcastle disease virus (NDV). A fusion phage carrying the amino acid sequence TLTTKLY was selected from the panning procedure. An antibody competition assay showed that the selected phage was capable of competing with the polyclonal antibodies raised against NDV for binding sites on the virus. Determination of the binding affinity of this phage with NDV by an equilibrium binding assay in solution revealed two different dissociation constants, suggesting that there could be two distinct binding sites for the phage on NDV. Synthetic peptides with the sequence CTLTTKLYC, either in linear or cyclic conformations inhibited the binding of phage bearing the same sequence to NDV. These peptides also inhibited the hemolytic activity of the virus as well as its propagation in embryonated chicken eggs.
format Article
author Ramanujam, P.
Tan, W. S.
Nathan, S.
Yusoff, K.
spellingShingle Ramanujam, P.
Tan, W. S.
Nathan, S.
Yusoff, K.
Novel peptides that inhibit the propagation of Newcastle disease virus
author_facet Ramanujam, P.
Tan, W. S.
Nathan, S.
Yusoff, K.
author_sort Ramanujam, P.
title Novel peptides that inhibit the propagation of Newcastle disease virus
title_short Novel peptides that inhibit the propagation of Newcastle disease virus
title_full Novel peptides that inhibit the propagation of Newcastle disease virus
title_fullStr Novel peptides that inhibit the propagation of Newcastle disease virus
title_full_unstemmed Novel peptides that inhibit the propagation of Newcastle disease virus
title_sort novel peptides that inhibit the propagation of newcastle disease virus
publisher Springer
publishDate 2002
url http://psasir.upm.edu.my/id/eprint/111867/
https://link.springer.com/article/10.1007/s00705-001-0778-y?error=cookies_not_supported&code=4d674463-e51e-4a15-a6d0-a442fc159735
_version_ 1825810702671020032
score 13.244413

Similar Items