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Isolation of microsatellite markers in mungbean, Vigna radiata

A simple and rapid method for isolating microsatellite loci in mungbean, Vigna radiata,based on the 5′′′′-anchored polymerase chain reaction technique revealed 23 microsatelliteloci and six cryptically simple sequence repeats. We report on the characterization of sevenpolymorphic microsatellite loci...

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Main Authors: Kumar, S. V., Tan, S. G., Quah, S. C., Yusoff, K.
Format: Article
Language:English
Published: Wiley 2002
Online Access:http://psasir.upm.edu.my/id/eprint/111737/3/111737.pdf
http://psasir.upm.edu.my/id/eprint/111737/
https://onlinelibrary.wiley.com/doi/10.1046/j.1471-8286.2002.00158.x
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spelling my.upm.eprints.1117372025-03-03T07:51:08Z http://psasir.upm.edu.my/id/eprint/111737/ Isolation of microsatellite markers in mungbean, Vigna radiata Kumar, S. V. Tan, S. G. Quah, S. C. Yusoff, K. A simple and rapid method for isolating microsatellite loci in mungbean, Vigna radiata,based on the 5′′′′-anchored polymerase chain reaction technique revealed 23 microsatelliteloci and six cryptically simple sequence repeats. We report on the characterization of sevenpolymorphic microsatellite loci in V. radiata. The number of alleles per locus ranged from2 to 5 while the observed heterozygosity ranged from 0 to 0.9048. These markers shouldprove useful as tools for detecting genetic variation in mungbean varieties for germplasmmanagement and crossbreeding purposes. Wiley 2002 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/111737/3/111737.pdf Kumar, S. V. and Tan, S. G. and Quah, S. C. and Yusoff, K. (2002) Isolation of microsatellite markers in mungbean, Vigna radiata. Molecular Ecology Notes, 2 (2). pp. 96-98. ISSN 1471-8278 https://onlinelibrary.wiley.com/doi/10.1046/j.1471-8286.2002.00158.x 10.1046/j.1471-8286.2002.00158.x
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description A simple and rapid method for isolating microsatellite loci in mungbean, Vigna radiata,based on the 5′′′′-anchored polymerase chain reaction technique revealed 23 microsatelliteloci and six cryptically simple sequence repeats. We report on the characterization of sevenpolymorphic microsatellite loci in V. radiata. The number of alleles per locus ranged from2 to 5 while the observed heterozygosity ranged from 0 to 0.9048. These markers shouldprove useful as tools for detecting genetic variation in mungbean varieties for germplasmmanagement and crossbreeding purposes.
format Article
author Kumar, S. V.
Tan, S. G.
Quah, S. C.
Yusoff, K.
spellingShingle Kumar, S. V.
Tan, S. G.
Quah, S. C.
Yusoff, K.
Isolation of microsatellite markers in mungbean, Vigna radiata
author_facet Kumar, S. V.
Tan, S. G.
Quah, S. C.
Yusoff, K.
author_sort Kumar, S. V.
title Isolation of microsatellite markers in mungbean, Vigna radiata
title_short Isolation of microsatellite markers in mungbean, Vigna radiata
title_full Isolation of microsatellite markers in mungbean, Vigna radiata
title_fullStr Isolation of microsatellite markers in mungbean, Vigna radiata
title_full_unstemmed Isolation of microsatellite markers in mungbean, Vigna radiata
title_sort isolation of microsatellite markers in mungbean, vigna radiata
publisher Wiley
publishDate 2002
url http://psasir.upm.edu.my/id/eprint/111737/3/111737.pdf
http://psasir.upm.edu.my/id/eprint/111737/
https://onlinelibrary.wiley.com/doi/10.1046/j.1471-8286.2002.00158.x
_version_ 1825810700856983552
score 13.244413

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