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Hematological, biochemical, and histopathological evaluation of the Morus alba L. leaf extract from Brunei Darussalam: acute toxicity study in ICR mice

Background: Studies have reported that the phytochemical content of Mulberry (Morus alba Linn.) is influenced by the area where it grows. On the other hand, the study of the bioactivity and toxicity of mulberry leaves from Brunei Darussalam still needs to be completed. In particular, the investigati...

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Main Authors: Fauzi, Ahmad, Kifli, Nurolaini, Noor, Mohd, Hamzah, Hazilawati, Azlan, Azrina
Format: Article
Language:English
Published: ScopeMed 2024
Online Access:http://psasir.upm.edu.my/id/eprint/111523/1/OVJ-%20Morus%20alba-%20paper%201.pdf
http://psasir.upm.edu.my/id/eprint/111523/
https://www.ejmanager.com/fulltextpdf.php?mno=165489
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spelling my.upm.eprints.1115232024-07-29T08:41:36Z http://psasir.upm.edu.my/id/eprint/111523/ Hematological, biochemical, and histopathological evaluation of the Morus alba L. leaf extract from Brunei Darussalam: acute toxicity study in ICR mice Fauzi, Ahmad Kifli, Nurolaini Noor, Mohd Hamzah, Hazilawati Azlan, Azrina Background: Studies have reported that the phytochemical content of Mulberry (Morus alba Linn.) is influenced by the area where it grows. On the other hand, the study of the bioactivity and toxicity of mulberry leaves from Brunei Darussalam still needs to be completed. In particular, the investigation regarding the safe dose for Mulberry’s application from Brunei Darussalam has yet to be studied. Hence, toxicity information must be considered even though the community has used it for generations. Aim: This study investigated Morus alba ethanolic leaf extract (MAE) to observe the acute toxicity in mice. Methods: In particular, this study utilized 12 female Institute of Cancer Research mice, 8 weeks old, divided into 2 groups: the control group and the MAE group (2,000 mg/kg single dose). Physiology, hematology, biochemistry, and histology were analyzed during the study. Results: The examination result indicated no mortality and behavioral changes throughout the testing period. However, the mice developed mild anemia and leukopenia, followed by decreased numbers of neutrophils, lymphocytes, and monocytes. In addition, the mice developed a mild hepatocellular injury, indicated by significant (p < 0.05) elevations of both alanine aminotransferase (ALT) and aspartate transaminase (AST). The histopathological findings of the liver were also consistent with the increment of ALT and AST, indicating mild hepatocellular necrosis through the eosinophilic cytoplasm and pyknosis (p > 0.05). Conclusion: It was evident that a single oral administration of MAE was not lethal for mice (LD50, which was higher than 2,000 mg/kg). However, the administration of high doses of MAE must be carefully considered. ScopeMed 2024 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/111523/1/OVJ-%20Morus%20alba-%20paper%201.pdf Fauzi, Ahmad and Kifli, Nurolaini and Noor, Mohd and Hamzah, Hazilawati and Azlan, Azrina (2024) Hematological, biochemical, and histopathological evaluation of the Morus alba L. leaf extract from Brunei Darussalam: acute toxicity study in ICR mice. Open Veterinary Journal, 14 (3). pp. 750-758. ISSN 22264485; EISSN: 22186050 https://www.ejmanager.com/fulltextpdf.php?mno=165489 10.5455/ovj.2024.v14.i3.1
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Background: Studies have reported that the phytochemical content of Mulberry (Morus alba Linn.) is influenced by the area where it grows. On the other hand, the study of the bioactivity and toxicity of mulberry leaves from Brunei Darussalam still needs to be completed. In particular, the investigation regarding the safe dose for Mulberry’s application from Brunei Darussalam has yet to be studied. Hence, toxicity information must be considered even though the community has used it for generations. Aim: This study investigated Morus alba ethanolic leaf extract (MAE) to observe the acute toxicity in mice. Methods: In particular, this study utilized 12 female Institute of Cancer Research mice, 8 weeks old, divided into 2 groups: the control group and the MAE group (2,000 mg/kg single dose). Physiology, hematology, biochemistry, and histology were analyzed during the study. Results: The examination result indicated no mortality and behavioral changes throughout the testing period. However, the mice developed mild anemia and leukopenia, followed by decreased numbers of neutrophils, lymphocytes, and monocytes. In addition, the mice developed a mild hepatocellular injury, indicated by significant (p < 0.05) elevations of both alanine aminotransferase (ALT) and aspartate transaminase (AST). The histopathological findings of the liver were also consistent with the increment of ALT and AST, indicating mild hepatocellular necrosis through the eosinophilic cytoplasm and pyknosis (p > 0.05). Conclusion: It was evident that a single oral administration of MAE was not lethal for mice (LD50, which was higher than 2,000 mg/kg). However, the administration of high doses of MAE must be carefully considered.
format Article
author Fauzi, Ahmad
Kifli, Nurolaini
Noor, Mohd
Hamzah, Hazilawati
Azlan, Azrina
spellingShingle Fauzi, Ahmad
Kifli, Nurolaini
Noor, Mohd
Hamzah, Hazilawati
Azlan, Azrina
Hematological, biochemical, and histopathological evaluation of the Morus alba L. leaf extract from Brunei Darussalam: acute toxicity study in ICR mice
author_facet Fauzi, Ahmad
Kifli, Nurolaini
Noor, Mohd
Hamzah, Hazilawati
Azlan, Azrina
author_sort Fauzi, Ahmad
title Hematological, biochemical, and histopathological evaluation of the Morus alba L. leaf extract from Brunei Darussalam: acute toxicity study in ICR mice
title_short Hematological, biochemical, and histopathological evaluation of the Morus alba L. leaf extract from Brunei Darussalam: acute toxicity study in ICR mice
title_full Hematological, biochemical, and histopathological evaluation of the Morus alba L. leaf extract from Brunei Darussalam: acute toxicity study in ICR mice
title_fullStr Hematological, biochemical, and histopathological evaluation of the Morus alba L. leaf extract from Brunei Darussalam: acute toxicity study in ICR mice
title_full_unstemmed Hematological, biochemical, and histopathological evaluation of the Morus alba L. leaf extract from Brunei Darussalam: acute toxicity study in ICR mice
title_sort hematological, biochemical, and histopathological evaluation of the morus alba l. leaf extract from brunei darussalam: acute toxicity study in icr mice
publisher ScopeMed
publishDate 2024
url http://psasir.upm.edu.my/id/eprint/111523/1/OVJ-%20Morus%20alba-%20paper%201.pdf
http://psasir.upm.edu.my/id/eprint/111523/
https://www.ejmanager.com/fulltextpdf.php?mno=165489
_version_ 1806415622533283840
score 13.211869

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