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Optimization of ionic liquid-based aqueous two-phase system for partial purification of bacteriocin-like inhibitory substance from Enterococcus faecium CC2 and its inhibitory activity against Streptococcus mutans

Background: Bacteriocin-like inhibitory substance (BLIS) demonstrates potent inhibitory effect against oral pathogens. To apply in oral care products, high purity of BLIS is required in view of the safety and effectiveness in the application. Therefore, aqueous two-phase systems (ATPS) are introduce...

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Main Authors: Ng, Zhang Jin, Ng, Hui Suan, Kee, Phei Er, Gan, Chee-Yuen, Ahmad Mokhtar, Ana Masara, Abu Zarin, Mazni, Lee, Chee Keong, Tan, Joo Shun, Lan, John Chi-Wei
Format: Article
Language:English
Published: Elsevier 2024
Online Access:http://psasir.upm.edu.my/id/eprint/111337/1/1-s2.0-S1876107024000063-main.pdf
http://psasir.upm.edu.my/id/eprint/111337/
https://linkinghub.elsevier.com/retrieve/pii/S1876107024000063
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spelling my.upm.eprints.1113372024-07-10T07:53:30Z http://psasir.upm.edu.my/id/eprint/111337/ Optimization of ionic liquid-based aqueous two-phase system for partial purification of bacteriocin-like inhibitory substance from Enterococcus faecium CC2 and its inhibitory activity against Streptococcus mutans Ng, Zhang Jin Ng, Hui Suan Kee, Phei Er Gan, Chee-Yuen Ahmad Mokhtar, Ana Masara Abu Zarin, Mazni Lee, Chee Keong Tan, Joo Shun Lan, John Chi-Wei Background: Bacteriocin-like inhibitory substance (BLIS) demonstrates potent inhibitory effect against oral pathogens. To apply in oral care products, high purity of BLIS is required in view of the safety and effectiveness in the application. Therefore, aqueous two-phase systems (ATPS) are introduced for the recovery and purification of BLIS. Methods: The optimization of ionic liquid-based ATPS (ILATPS) for the purification of BLIS from Enterococcus faecium CC2 was performed using response surface methodology (RSM). The partially purified BLIS was then identified, and the inhibitory effect against Streptococcus mutans UKMCC1019 was assessed. Significant Findings: ILATPS with a pH of 7 at a temperature of 40 °C, comprising of 10 % (w/w) fermentation broth, 15 % (w/w) 1‑butyl‑3-methylimidazolium tetrafluoroborate, (Bmim)BF4, 12.5 % (w/w) sodium citrate and 3 % (w/w) sodium chloride, resulted in the optimum purification factor of 4.406, recovery yield of 67.213 % and partition coefficient of 4.852. The BLIS was identified as enterocin CC2, with a molecular size of approximately 5.5–6 kDa. Furthermore, the partially purified BLIS at a concentration of 1.00 mg/mL demonstrated effective growth inhibition against S. mutans UKMCC1019. The ILATPS was shown to be a potential technology to enhance the purity of BLIS from E. faecium CC2. Elsevier 2024 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/111337/1/1-s2.0-S1876107024000063-main.pdf Ng, Zhang Jin and Ng, Hui Suan and Kee, Phei Er and Gan, Chee-Yuen and Ahmad Mokhtar, Ana Masara and Abu Zarin, Mazni and Lee, Chee Keong and Tan, Joo Shun and Lan, John Chi-Wei (2024) Optimization of ionic liquid-based aqueous two-phase system for partial purification of bacteriocin-like inhibitory substance from Enterococcus faecium CC2 and its inhibitory activity against Streptococcus mutans. Journal of the Taiwan Institute of Chemical Engineers, 160. art. no. 105347. pp. 1-9. ISSN 1876-1070; ESSN: 1876-1089 https://linkinghub.elsevier.com/retrieve/pii/S1876107024000063 10.1016/j.jtice.2024.105347
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Background: Bacteriocin-like inhibitory substance (BLIS) demonstrates potent inhibitory effect against oral pathogens. To apply in oral care products, high purity of BLIS is required in view of the safety and effectiveness in the application. Therefore, aqueous two-phase systems (ATPS) are introduced for the recovery and purification of BLIS. Methods: The optimization of ionic liquid-based ATPS (ILATPS) for the purification of BLIS from Enterococcus faecium CC2 was performed using response surface methodology (RSM). The partially purified BLIS was then identified, and the inhibitory effect against Streptococcus mutans UKMCC1019 was assessed. Significant Findings: ILATPS with a pH of 7 at a temperature of 40 °C, comprising of 10 % (w/w) fermentation broth, 15 % (w/w) 1‑butyl‑3-methylimidazolium tetrafluoroborate, (Bmim)BF4, 12.5 % (w/w) sodium citrate and 3 % (w/w) sodium chloride, resulted in the optimum purification factor of 4.406, recovery yield of 67.213 % and partition coefficient of 4.852. The BLIS was identified as enterocin CC2, with a molecular size of approximately 5.5–6 kDa. Furthermore, the partially purified BLIS at a concentration of 1.00 mg/mL demonstrated effective growth inhibition against S. mutans UKMCC1019. The ILATPS was shown to be a potential technology to enhance the purity of BLIS from E. faecium CC2.
format Article
author Ng, Zhang Jin
Ng, Hui Suan
Kee, Phei Er
Gan, Chee-Yuen
Ahmad Mokhtar, Ana Masara
Abu Zarin, Mazni
Lee, Chee Keong
Tan, Joo Shun
Lan, John Chi-Wei
spellingShingle Ng, Zhang Jin
Ng, Hui Suan
Kee, Phei Er
Gan, Chee-Yuen
Ahmad Mokhtar, Ana Masara
Abu Zarin, Mazni
Lee, Chee Keong
Tan, Joo Shun
Lan, John Chi-Wei
Optimization of ionic liquid-based aqueous two-phase system for partial purification of bacteriocin-like inhibitory substance from Enterococcus faecium CC2 and its inhibitory activity against Streptococcus mutans
author_facet Ng, Zhang Jin
Ng, Hui Suan
Kee, Phei Er
Gan, Chee-Yuen
Ahmad Mokhtar, Ana Masara
Abu Zarin, Mazni
Lee, Chee Keong
Tan, Joo Shun
Lan, John Chi-Wei
author_sort Ng, Zhang Jin
title Optimization of ionic liquid-based aqueous two-phase system for partial purification of bacteriocin-like inhibitory substance from Enterococcus faecium CC2 and its inhibitory activity against Streptococcus mutans
title_short Optimization of ionic liquid-based aqueous two-phase system for partial purification of bacteriocin-like inhibitory substance from Enterococcus faecium CC2 and its inhibitory activity against Streptococcus mutans
title_full Optimization of ionic liquid-based aqueous two-phase system for partial purification of bacteriocin-like inhibitory substance from Enterococcus faecium CC2 and its inhibitory activity against Streptococcus mutans
title_fullStr Optimization of ionic liquid-based aqueous two-phase system for partial purification of bacteriocin-like inhibitory substance from Enterococcus faecium CC2 and its inhibitory activity against Streptococcus mutans
title_full_unstemmed Optimization of ionic liquid-based aqueous two-phase system for partial purification of bacteriocin-like inhibitory substance from Enterococcus faecium CC2 and its inhibitory activity against Streptococcus mutans
title_sort optimization of ionic liquid-based aqueous two-phase system for partial purification of bacteriocin-like inhibitory substance from enterococcus faecium cc2 and its inhibitory activity against streptococcus mutans
publisher Elsevier
publishDate 2024
url http://psasir.upm.edu.my/id/eprint/111337/1/1-s2.0-S1876107024000063-main.pdf
http://psasir.upm.edu.my/id/eprint/111337/
https://linkinghub.elsevier.com/retrieve/pii/S1876107024000063
_version_ 1805889533355491328
score 13.211869

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