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In vitro antigenicity and cross-reaction of the outer membrane proteins of Pasteurella haemolytica A2, A7 and A9

The outer membrane proteins of Pasteurella haemolytica A2, A7 and A9 were subjected to SDS-PAGE and immunoblotting. The molecular weights of the polypeptide bands ranged between 33 to 97 kDa. The major polypeptide bands for P. haemolytica A2 were 33.4, 39.2 and 45 kDa while the minor polypeptide ban...

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Main Authors: Mohd Yusoff, Md Sabri, Saad, Mohd Zamri, Mutalib, Abdul Rahim, Israf Ali, Daud Ahmad, N., Muniandy
Format: Article
Language:English
English
Published: 1999
Online Access:http://psasir.upm.edu.my/id/eprint/11043/1/In%20vitro%20antigenicity%20and%20cross.pdf
http://psasir.upm.edu.my/id/eprint/11043/
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spelling my.upm.eprints.110432015-10-26T04:12:47Z http://psasir.upm.edu.my/id/eprint/11043/ In vitro antigenicity and cross-reaction of the outer membrane proteins of Pasteurella haemolytica A2, A7 and A9 Mohd Yusoff, Md Sabri Saad, Mohd Zamri Mutalib, Abdul Rahim Israf Ali, Daud Ahmad N., Muniandy The outer membrane proteins of Pasteurella haemolytica A2, A7 and A9 were subjected to SDS-PAGE and immunoblotting. The molecular weights of the polypeptide bands ranged between 33 to 97 kDa. The major polypeptide bands for P. haemolytica A2 were 33.4, 39.2 and 45 kDa while the minor polypeptide bands were 50, 58.7, 66.2, 84.7 and 97.4 kDa. Analysis of the outer membrane proteins of P. haemolytica A7 revealed two major protein bands of 33.4 and 45 kDa and three minor polypeptide of 40, 50 and 66.2 kDa. There were three major (33.4, 37.5 and 45 kDa) and one minor protein band (50 kDa) in the outer membrane proteins of P. haemolytica A9. There was one major protein band from each of the P. haemolytica A2, A7 and A9, which was unique to the respective serotype and appeared to represent the respective serotype. These were the 39.2 kDa band for P. haemolytica A2, the 40 kDa band for P. haemolytica A7 and the 37.5 kDa band for P. haemolytica A9. Following homologous immunoblot, all the serotypes showed pronounced antigenicity at the 30 kDa band. Heterologous immunoblot using the antiserum of P. haemolytica A2 did not reveal any antigenic band of P. haemolytica A9 but revealed antigenic bands at 30 and 31 kDa of P. haemolytica A7. Heterologous immunoblot using the antiserum of P. haemolytica A7 revealed antigenic band at 30 kDa of all the three serotypes while the antiserum of P. haemolytica A9 failed to reveal any common antigenic band between all three serotypes. Thus, the 30 kDa band of P. haemolytica A7 may be a suitable candidate for a sub-unit Vaccine against pneumonic pasteurellosis of sheep and goats. 1999-12 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/11043/1/In%20vitro%20antigenicity%20and%20cross.pdf Mohd Yusoff, Md Sabri and Saad, Mohd Zamri and Mutalib, Abdul Rahim and Israf Ali, Daud Ahmad and N., Muniandy (1999) In vitro antigenicity and cross-reaction of the outer membrane proteins of Pasteurella haemolytica A2, A7 and A9. Asia-Pacific Journal of Molecular Biology and Biotechnology, 7 (2). pp. 135-141. ISSN 0128-7451 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description The outer membrane proteins of Pasteurella haemolytica A2, A7 and A9 were subjected to SDS-PAGE and immunoblotting. The molecular weights of the polypeptide bands ranged between 33 to 97 kDa. The major polypeptide bands for P. haemolytica A2 were 33.4, 39.2 and 45 kDa while the minor polypeptide bands were 50, 58.7, 66.2, 84.7 and 97.4 kDa. Analysis of the outer membrane proteins of P. haemolytica A7 revealed two major protein bands of 33.4 and 45 kDa and three minor polypeptide of 40, 50 and 66.2 kDa. There were three major (33.4, 37.5 and 45 kDa) and one minor protein band (50 kDa) in the outer membrane proteins of P. haemolytica A9. There was one major protein band from each of the P. haemolytica A2, A7 and A9, which was unique to the respective serotype and appeared to represent the respective serotype. These were the 39.2 kDa band for P. haemolytica A2, the 40 kDa band for P. haemolytica A7 and the 37.5 kDa band for P. haemolytica A9. Following homologous immunoblot, all the serotypes showed pronounced antigenicity at the 30 kDa band. Heterologous immunoblot using the antiserum of P. haemolytica A2 did not reveal any antigenic band of P. haemolytica A9 but revealed antigenic bands at 30 and 31 kDa of P. haemolytica A7. Heterologous immunoblot using the antiserum of P. haemolytica A7 revealed antigenic band at 30 kDa of all the three serotypes while the antiserum of P. haemolytica A9 failed to reveal any common antigenic band between all three serotypes. Thus, the 30 kDa band of P. haemolytica A7 may be a suitable candidate for a sub-unit Vaccine against pneumonic pasteurellosis of sheep and goats.
format Article
author Mohd Yusoff, Md Sabri
Saad, Mohd Zamri
Mutalib, Abdul Rahim
Israf Ali, Daud Ahmad
N., Muniandy
spellingShingle Mohd Yusoff, Md Sabri
Saad, Mohd Zamri
Mutalib, Abdul Rahim
Israf Ali, Daud Ahmad
N., Muniandy
In vitro antigenicity and cross-reaction of the outer membrane proteins of Pasteurella haemolytica A2, A7 and A9
author_facet Mohd Yusoff, Md Sabri
Saad, Mohd Zamri
Mutalib, Abdul Rahim
Israf Ali, Daud Ahmad
N., Muniandy
author_sort Mohd Yusoff, Md Sabri
title In vitro antigenicity and cross-reaction of the outer membrane proteins of Pasteurella haemolytica A2, A7 and A9
title_short In vitro antigenicity and cross-reaction of the outer membrane proteins of Pasteurella haemolytica A2, A7 and A9
title_full In vitro antigenicity and cross-reaction of the outer membrane proteins of Pasteurella haemolytica A2, A7 and A9
title_fullStr In vitro antigenicity and cross-reaction of the outer membrane proteins of Pasteurella haemolytica A2, A7 and A9
title_full_unstemmed In vitro antigenicity and cross-reaction of the outer membrane proteins of Pasteurella haemolytica A2, A7 and A9
title_sort in vitro antigenicity and cross-reaction of the outer membrane proteins of pasteurella haemolytica a2, a7 and a9
publishDate 1999
url http://psasir.upm.edu.my/id/eprint/11043/1/In%20vitro%20antigenicity%20and%20cross.pdf
http://psasir.upm.edu.my/id/eprint/11043/
_version_ 1643824727021584384
score 13.244413

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