Cover Image

Mining the genome of Bacillus velezensis FS26 for probiotic markers and secondary metabolites with antimicrobial properties against aquaculture pathogens

Bacillus velezensis FS26 is a bacterium from the genus Bacillus that has been proven as a potential probiotic in aquaculture with a good antagonistic effect on Aeromonas spp. and Vibrio spp. Whole-genome sequencing (WGS) allows a comprehensive and in-depth analysis at the molecular level, and it is...

Full description

Saved in:
Bibliographic Details
Main Authors: Sam-on, Muhamad Firdaus Syahmi, Mustafa, Shuhaimi, Mohd Hashim, Amalia, Yusof, Mohd Termizi, Zulkifly, Shahrizim, Malek, Ahmad Zuhairi Abdul, Roslan, Muhamad Akhmal Hakim, Mohd Asrore, Mohd Shaufi
Format: Article
Language:English
Published: Elsevier BV 2023
Online Access:http://psasir.upm.edu.my/id/eprint/110404/1/110404.pdf
http://psasir.upm.edu.my/id/eprint/110404/
https://www.sciencedirect.com/science/article/pii/S0882401023001948
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.110404
record_format eprints
spelling my.upm.eprints.1104042024-10-08T06:18:44Z http://psasir.upm.edu.my/id/eprint/110404/ Mining the genome of Bacillus velezensis FS26 for probiotic markers and secondary metabolites with antimicrobial properties against aquaculture pathogens Sam-on, Muhamad Firdaus Syahmi Mustafa, Shuhaimi Mohd Hashim, Amalia Yusof, Mohd Termizi Zulkifly, Shahrizim Malek, Ahmad Zuhairi Abdul Roslan, Muhamad Akhmal Hakim Mohd Asrore, Mohd Shaufi Bacillus velezensis FS26 is a bacterium from the genus Bacillus that has been proven as a potential probiotic in aquaculture with a good antagonistic effect on Aeromonas spp. and Vibrio spp. Whole-genome sequencing (WGS) allows a comprehensive and in-depth analysis at the molecular level, and it is becoming an increasingly significant technique in aquaculture research. Although numerous probiotic genomes have been sequenced and investigated recently, there are minimal data on in silico analysis of B. velezensis as a probiotic bacterium isolated from aquaculture sources. Thus, this study aims to analyse the general genome characteristics and probiotic markers from the B. velezensis FS26 genome with secondary metabolites predicted against aquaculture pathogens. The B. velezensis FS26 genome (GenBank Accession: JAOPEO000000000) assembly proved to be of high quality, with eight contigs containing 3,926,371 bp and an average G + C content of 46.5. According to antiSMASH analysis, five clusters of secondary metabolites from the B. velezensis FS26 genome showed 100 similarity. These clusters include Cluster 2 (bacilysin), Cluster 6 (bacillibactin), Cluster 7 (fengycin), Cluster 8 (bacillaene), and Cluster 9 (macrolactin H), which signify promising antibacterial, antifungal, and anticyanobacterial agents against pathogens in aquaculture. The probiotic markers of B. velezensis FS26 genome for adhesion capability in the hosts’ intestine, as well as the acid and bile salt-tolerant genes, were also detected through the Prokaryotic Genome Annotation System (Prokka) annotation pipeline. These results are in agreement with our previous in vitro data, suggesting that the in silico investigation facilitates establishing B. velezensis FS26 as a beneficial probiotic for use in aquaculture. Elsevier BV 2023-08 Article NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/110404/1/110404.pdf Sam-on, Muhamad Firdaus Syahmi and Mustafa, Shuhaimi and Mohd Hashim, Amalia and Yusof, Mohd Termizi and Zulkifly, Shahrizim and Malek, Ahmad Zuhairi Abdul and Roslan, Muhamad Akhmal Hakim and Mohd Asrore, Mohd Shaufi (2023) Mining the genome of Bacillus velezensis FS26 for probiotic markers and secondary metabolites with antimicrobial properties against aquaculture pathogens. Microbial Pathogenesis, 181. art. no. 106161. pp. 1-7. ISSN 0882-4010 https://www.sciencedirect.com/science/article/pii/S0882401023001948 10.1016/j.micpath.2023.106161
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Bacillus velezensis FS26 is a bacterium from the genus Bacillus that has been proven as a potential probiotic in aquaculture with a good antagonistic effect on Aeromonas spp. and Vibrio spp. Whole-genome sequencing (WGS) allows a comprehensive and in-depth analysis at the molecular level, and it is becoming an increasingly significant technique in aquaculture research. Although numerous probiotic genomes have been sequenced and investigated recently, there are minimal data on in silico analysis of B. velezensis as a probiotic bacterium isolated from aquaculture sources. Thus, this study aims to analyse the general genome characteristics and probiotic markers from the B. velezensis FS26 genome with secondary metabolites predicted against aquaculture pathogens. The B. velezensis FS26 genome (GenBank Accession: JAOPEO000000000) assembly proved to be of high quality, with eight contigs containing 3,926,371 bp and an average G + C content of 46.5. According to antiSMASH analysis, five clusters of secondary metabolites from the B. velezensis FS26 genome showed 100 similarity. These clusters include Cluster 2 (bacilysin), Cluster 6 (bacillibactin), Cluster 7 (fengycin), Cluster 8 (bacillaene), and Cluster 9 (macrolactin H), which signify promising antibacterial, antifungal, and anticyanobacterial agents against pathogens in aquaculture. The probiotic markers of B. velezensis FS26 genome for adhesion capability in the hosts’ intestine, as well as the acid and bile salt-tolerant genes, were also detected through the Prokaryotic Genome Annotation System (Prokka) annotation pipeline. These results are in agreement with our previous in vitro data, suggesting that the in silico investigation facilitates establishing B. velezensis FS26 as a beneficial probiotic for use in aquaculture.
format Article
author Sam-on, Muhamad Firdaus Syahmi
Mustafa, Shuhaimi
Mohd Hashim, Amalia
Yusof, Mohd Termizi
Zulkifly, Shahrizim
Malek, Ahmad Zuhairi Abdul
Roslan, Muhamad Akhmal Hakim
Mohd Asrore, Mohd Shaufi
spellingShingle Sam-on, Muhamad Firdaus Syahmi
Mustafa, Shuhaimi
Mohd Hashim, Amalia
Yusof, Mohd Termizi
Zulkifly, Shahrizim
Malek, Ahmad Zuhairi Abdul
Roslan, Muhamad Akhmal Hakim
Mohd Asrore, Mohd Shaufi
Mining the genome of Bacillus velezensis FS26 for probiotic markers and secondary metabolites with antimicrobial properties against aquaculture pathogens
author_facet Sam-on, Muhamad Firdaus Syahmi
Mustafa, Shuhaimi
Mohd Hashim, Amalia
Yusof, Mohd Termizi
Zulkifly, Shahrizim
Malek, Ahmad Zuhairi Abdul
Roslan, Muhamad Akhmal Hakim
Mohd Asrore, Mohd Shaufi
author_sort Sam-on, Muhamad Firdaus Syahmi
title Mining the genome of Bacillus velezensis FS26 for probiotic markers and secondary metabolites with antimicrobial properties against aquaculture pathogens
title_short Mining the genome of Bacillus velezensis FS26 for probiotic markers and secondary metabolites with antimicrobial properties against aquaculture pathogens
title_full Mining the genome of Bacillus velezensis FS26 for probiotic markers and secondary metabolites with antimicrobial properties against aquaculture pathogens
title_fullStr Mining the genome of Bacillus velezensis FS26 for probiotic markers and secondary metabolites with antimicrobial properties against aquaculture pathogens
title_full_unstemmed Mining the genome of Bacillus velezensis FS26 for probiotic markers and secondary metabolites with antimicrobial properties against aquaculture pathogens
title_sort mining the genome of bacillus velezensis fs26 for probiotic markers and secondary metabolites with antimicrobial properties against aquaculture pathogens
publisher Elsevier BV
publishDate 2023
url http://psasir.upm.edu.my/id/eprint/110404/1/110404.pdf
http://psasir.upm.edu.my/id/eprint/110404/
https://www.sciencedirect.com/science/article/pii/S0882401023001948
_version_ 1814054705506025472
score 13.211869

Similar Items