Staff View: Pyriproxyfen influences growth as well as thyroid hormone–related and gh/ igf-1 gene expression during the early life stage of zebrafish (danio rerio)

Pyriproxyfen influences growth as well as thyroid hormone–related and gh/ igf-1 gene expression during the early life stage of zebrafish (danio rerio)

Pyriproxyfen is an agricultural chemical pesticide that has been detected in the aquatic environment. This study aimed to clarify the effects of pyriproxyfen on the growth as well as thyroid hormone– and growth-related gene expression of zebrafish (Danio rerio) during its early life stage. Pyriproxy...

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Main Authors:	Horie, Yoshifumi, Mitsunaga, Kensuke, Chee, Kong Yap
Format:	Article
Published:	Elsevier BV 2023
Online Access:	http://psasir.upm.edu.my/id/eprint/110249/ https://linkinghub.elsevier.com/retrieve/pii/S153204562300087X
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id	my.upm.eprints.110249
record_format	eprints
spelling	my.upm.eprints.1102492024-09-03T07:29:31Z http://psasir.upm.edu.my/id/eprint/110249/ Pyriproxyfen influences growth as well as thyroid hormone–related and gh/ igf-1 gene expression during the early life stage of zebrafish (danio rerio) Horie, Yoshifumi Mitsunaga, Kensuke Chee, Kong Yap Pyriproxyfen is an agricultural chemical pesticide that has been detected in the aquatic environment. This study aimed to clarify the effects of pyriproxyfen on the growth as well as thyroid hormone– and growth-related gene expression of zebrafish (Danio rerio) during its early life stage. Pyriproxyfen exhibited a lethal effect in a concentration-dependent manner: the lowest and no effect concentrations were 250.7 and 111.7 μg/L, respectively. These concentrations were considerably higher than the residual environmental concentrations, indicating the low risk of this pesticide when present at such concentrations. In the zebrafish group treated with 56.6 μg/L pyriproxyfen, thyroid hormone receptor β gene expression levels remained unchanged, whereas thyroidstimulating hormone β subunit, iodtyronin deiodinase 2, and thyroid hormone receptor α gene expression levels significantly decreased compared with the control group expression levels. In zebrafish treated with 111.7 or 250.7 μg/L pyriproxyfen, iodtyronin deiodinase 1 gene expression levels significantly increased. These results indicate that pyriproxyfen disrupts thyroid hormone activity in zebrafish. Furthermore, pyriproxyfen exposure inhibited zebrafish growth; consequently, we examined the expression of growth hormone (gh) and insulin-like growth factor-I (igf-1), which are important for growth. Pyriproxyfen exposure suppressed gh expression; however, the igf-1 expression levels remained unchanged. Therefore, growth inhibition due to pyriproxyfen exposure was attributed to the suppression of gh expression. Elsevier BV 2023 Article PeerReviewed Horie, Yoshifumi and Mitsunaga, Kensuke and Chee, Kong Yap (2023) Pyriproxyfen influences growth as well as thyroid hormone–related and gh/ igf-1 gene expression during the early life stage of zebrafish (danio rerio). Comparative Biochemistry and Physiology, Part C, 269. art. no. 109632. pp. 1-6. ISSN 1532-0456; ESSN: 1878-1659 https://linkinghub.elsevier.com/retrieve/pii/S153204562300087X 10.1016/j.cbpc.2023.109632
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
description	Pyriproxyfen is an agricultural chemical pesticide that has been detected in the aquatic environment. This study aimed to clarify the effects of pyriproxyfen on the growth as well as thyroid hormone– and growth-related gene expression of zebrafish (Danio rerio) during its early life stage. Pyriproxyfen exhibited a lethal effect in a concentration-dependent manner: the lowest and no effect concentrations were 250.7 and 111.7 μg/L, respectively. These concentrations were considerably higher than the residual environmental concentrations, indicating the low risk of this pesticide when present at such concentrations. In the zebrafish group treated with 56.6 μg/L pyriproxyfen, thyroid hormone receptor β gene expression levels remained unchanged, whereas thyroidstimulating hormone β subunit, iodtyronin deiodinase 2, and thyroid hormone receptor α gene expression levels significantly decreased compared with the control group expression levels. In zebrafish treated with 111.7 or 250.7 μg/L pyriproxyfen, iodtyronin deiodinase 1 gene expression levels significantly increased. These results indicate that pyriproxyfen disrupts thyroid hormone activity in zebrafish. Furthermore, pyriproxyfen exposure inhibited zebrafish growth; consequently, we examined the expression of growth hormone (gh) and insulin-like growth factor-I (igf-1), which are important for growth. Pyriproxyfen exposure suppressed gh expression; however, the igf-1 expression levels remained unchanged. Therefore, growth inhibition due to pyriproxyfen exposure was attributed to the suppression of gh expression.
format	Article
author	Horie, Yoshifumi Mitsunaga, Kensuke Chee, Kong Yap
spellingShingle	Horie, Yoshifumi Mitsunaga, Kensuke Chee, Kong Yap Pyriproxyfen influences growth as well as thyroid hormone–related and gh/ igf-1 gene expression during the early life stage of zebrafish (danio rerio)
author_facet	Horie, Yoshifumi Mitsunaga, Kensuke Chee, Kong Yap
author_sort	Horie, Yoshifumi
title	Pyriproxyfen influences growth as well as thyroid hormone–related and gh/ igf-1 gene expression during the early life stage of zebrafish (danio rerio)
title_short	Pyriproxyfen influences growth as well as thyroid hormone–related and gh/ igf-1 gene expression during the early life stage of zebrafish (danio rerio)
title_full	Pyriproxyfen influences growth as well as thyroid hormone–related and gh/ igf-1 gene expression during the early life stage of zebrafish (danio rerio)
title_fullStr	Pyriproxyfen influences growth as well as thyroid hormone–related and gh/ igf-1 gene expression during the early life stage of zebrafish (danio rerio)
title_full_unstemmed	Pyriproxyfen influences growth as well as thyroid hormone–related and gh/ igf-1 gene expression during the early life stage of zebrafish (danio rerio)
title_sort	pyriproxyfen influences growth as well as thyroid hormone–related and gh/ igf-1 gene expression during the early life stage of zebrafish (danio rerio)
publisher	Elsevier BV
publishDate	2023
url	http://psasir.upm.edu.my/id/eprint/110249/ https://linkinghub.elsevier.com/retrieve/pii/S153204562300087X
_version_	1811686062651604992
score	13.222552

Pyriproxyfen influences growth as well as thyroid hormone–related and gh/ igf-1 gene expression during the early life stage of zebrafish (danio rerio)

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