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Isolation and characterization of biohydrogen-producing bacteria for biohydrogen fermentation using oil palm biomass-based carbon source

The effectiveness of biohydrogen conversion from biomass sources is governed by the selection of ideal biohydrogen-producing bacteria to achieve high and consistent production performance. The aim of this research was to isolate and identify a biohydrogen producer in local soil samples, as well as t...

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Main Authors: Luthfi, Abdullah Amru Indera, Abdul, Peer Mohamed, Md Jahim, Jamaliah, Engliman, Nurul Sakinah, Jamali, Nur Syakina, Tan, Jian Ping, Abdul Manaf, Shareena Fairuz, Sajab, Mohd Shaiful, Bukhari, Nurul Adela
Format: Article
Language:English
Published: Multidisciplinary Digital Publishing Institute 2023
Online Access:http://psasir.upm.edu.my/id/eprint/109470/1/applsci-13-00656-v2.pdf
http://psasir.upm.edu.my/id/eprint/109470/
https://www.mdpi.com/2076-3417/13/1/656
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spelling my.upm.eprints.1094702024-12-10T03:31:24Z http://psasir.upm.edu.my/id/eprint/109470/ Isolation and characterization of biohydrogen-producing bacteria for biohydrogen fermentation using oil palm biomass-based carbon source Luthfi, Abdullah Amru Indera Abdul, Peer Mohamed Md Jahim, Jamaliah Engliman, Nurul Sakinah Jamali, Nur Syakina Tan, Jian Ping Abdul Manaf, Shareena Fairuz Sajab, Mohd Shaiful Bukhari, Nurul Adela The effectiveness of biohydrogen conversion from biomass sources is governed by the selection of ideal biohydrogen-producing bacteria to achieve high and consistent production performance. The aim of this research was to isolate and identify a biohydrogen producer in local soil samples, as well as to evaluate its fermentability in biohydrogen production from oil palm empty fruit bunches (OPEFB). To this end, preliminary identification was performed using morphological, phenotype, biological, and 16s rRNA analyses. The fermentability of the isolate was further evaluated in a serum bottle and then in a 1.5 L anaerobic column bioreactor (ACBR) to investigate the potential for biohydrogen production using two OPEFB-based carbon sources: hydrolysate of ammonia fiber expansion (AFEX)-pretreated OPEFB and molasses from dilute acetic acid (DAA)-pretreated OPEFB. The isolated strain, Enterobacter sp. KBH 6958, was found to be capable of producing biohydrogen from various carbon sources via the pyruvate:ferredoxin oxidoreductase (PFOR) pathway. The cumulative conversion of AFEX OPEFB hydrolysate was 45% higher than that observed in DAA OPEFB molasses fermentation in the production of biohydrogen. The biohydrogen yield after fermenting AFEX OPEFB hydrolysate with Enterobacter sp. KBH 6958 was 1.55 mol H2/mol sugar, with a maximum productivity of 98.1 mL H2/h (4.01 mmol H2/L/h), whereas butyrate (10.6 mM), acetate (11.8 mM), and ethanol (4.56 mM) were found to be the major soluble metabolites. This study successfully demonstrated the biotechnological conversion of OPEFB into biohydrogen using a locally isolated strain, which not only solves environmental issues associated with the industry but may also offer a solution to the world’s energy insecurity. Multidisciplinary Digital Publishing Institute 2023-01-03 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/109470/1/applsci-13-00656-v2.pdf Luthfi, Abdullah Amru Indera and Abdul, Peer Mohamed and Md Jahim, Jamaliah and Engliman, Nurul Sakinah and Jamali, Nur Syakina and Tan, Jian Ping and Abdul Manaf, Shareena Fairuz and Sajab, Mohd Shaiful and Bukhari, Nurul Adela (2023) Isolation and characterization of biohydrogen-producing bacteria for biohydrogen fermentation using oil palm biomass-based carbon source. Applied Sciences, 13 (1). pp. 1-18. ISSN 2076-3417 https://www.mdpi.com/2076-3417/13/1/656 10.3390/app13010656
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The effectiveness of biohydrogen conversion from biomass sources is governed by the selection of ideal biohydrogen-producing bacteria to achieve high and consistent production performance. The aim of this research was to isolate and identify a biohydrogen producer in local soil samples, as well as to evaluate its fermentability in biohydrogen production from oil palm empty fruit bunches (OPEFB). To this end, preliminary identification was performed using morphological, phenotype, biological, and 16s rRNA analyses. The fermentability of the isolate was further evaluated in a serum bottle and then in a 1.5 L anaerobic column bioreactor (ACBR) to investigate the potential for biohydrogen production using two OPEFB-based carbon sources: hydrolysate of ammonia fiber expansion (AFEX)-pretreated OPEFB and molasses from dilute acetic acid (DAA)-pretreated OPEFB. The isolated strain, Enterobacter sp. KBH 6958, was found to be capable of producing biohydrogen from various carbon sources via the pyruvate:ferredoxin oxidoreductase (PFOR) pathway. The cumulative conversion of AFEX OPEFB hydrolysate was 45% higher than that observed in DAA OPEFB molasses fermentation in the production of biohydrogen. The biohydrogen yield after fermenting AFEX OPEFB hydrolysate with Enterobacter sp. KBH 6958 was 1.55 mol H2/mol sugar, with a maximum productivity of 98.1 mL H2/h (4.01 mmol H2/L/h), whereas butyrate (10.6 mM), acetate (11.8 mM), and ethanol (4.56 mM) were found to be the major soluble metabolites. This study successfully demonstrated the biotechnological conversion of OPEFB into biohydrogen using a locally isolated strain, which not only solves environmental issues associated with the industry but may also offer a solution to the world’s energy insecurity.
format Article
author Luthfi, Abdullah Amru Indera
Abdul, Peer Mohamed
Md Jahim, Jamaliah
Engliman, Nurul Sakinah
Jamali, Nur Syakina
Tan, Jian Ping
Abdul Manaf, Shareena Fairuz
Sajab, Mohd Shaiful
Bukhari, Nurul Adela
spellingShingle Luthfi, Abdullah Amru Indera
Abdul, Peer Mohamed
Md Jahim, Jamaliah
Engliman, Nurul Sakinah
Jamali, Nur Syakina
Tan, Jian Ping
Abdul Manaf, Shareena Fairuz
Sajab, Mohd Shaiful
Bukhari, Nurul Adela
Isolation and characterization of biohydrogen-producing bacteria for biohydrogen fermentation using oil palm biomass-based carbon source
author_facet Luthfi, Abdullah Amru Indera
Abdul, Peer Mohamed
Md Jahim, Jamaliah
Engliman, Nurul Sakinah
Jamali, Nur Syakina
Tan, Jian Ping
Abdul Manaf, Shareena Fairuz
Sajab, Mohd Shaiful
Bukhari, Nurul Adela
author_sort Luthfi, Abdullah Amru Indera
title Isolation and characterization of biohydrogen-producing bacteria for biohydrogen fermentation using oil palm biomass-based carbon source
title_short Isolation and characterization of biohydrogen-producing bacteria for biohydrogen fermentation using oil palm biomass-based carbon source
title_full Isolation and characterization of biohydrogen-producing bacteria for biohydrogen fermentation using oil palm biomass-based carbon source
title_fullStr Isolation and characterization of biohydrogen-producing bacteria for biohydrogen fermentation using oil palm biomass-based carbon source
title_full_unstemmed Isolation and characterization of biohydrogen-producing bacteria for biohydrogen fermentation using oil palm biomass-based carbon source
title_sort isolation and characterization of biohydrogen-producing bacteria for biohydrogen fermentation using oil palm biomass-based carbon source
publisher Multidisciplinary Digital Publishing Institute
publishDate 2023
url http://psasir.upm.edu.my/id/eprint/109470/1/applsci-13-00656-v2.pdf
http://psasir.upm.edu.my/id/eprint/109470/
https://www.mdpi.com/2076-3417/13/1/656
_version_ 1818835876152606720
score 13.223943

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