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Antifungal potential of Melaleuca alternifolia against fungal pathogen Fusarium oxysporum f. sp. cubense Tropical Race 4

Fusarium wilt of bananas caused by Fusarium oxysporum f. sp. cubense Tropical Race 4 (Foc TR4) poses the most serious threat to banana production globally. The disease has been managed using chemical fungicides, yet the control levels are still unsatisfactory. This study investigated the antifungal...

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Main Authors: Paramalingam, Pavitra, Baharum, Nadiya Akmal, Abdullah, Janna Ong, Hong, Jeum Kyu, Saidi, Noor Baity
Format: Article
Published: Multidisciplinary Digital Publishing Institute 2023
Online Access:http://psasir.upm.edu.my/id/eprint/109374/
https://www.mdpi.com/1420-3049/28/11/4456
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spelling my.upm.eprints.1093742024-08-05T03:38:51Z http://psasir.upm.edu.my/id/eprint/109374/ Antifungal potential of Melaleuca alternifolia against fungal pathogen Fusarium oxysporum f. sp. cubense Tropical Race 4 Paramalingam, Pavitra Baharum, Nadiya Akmal Abdullah, Janna Ong Hong, Jeum Kyu Saidi, Noor Baity Fusarium wilt of bananas caused by Fusarium oxysporum f. sp. cubense Tropical Race 4 (Foc TR4) poses the most serious threat to banana production globally. The disease has been managed using chemical fungicides, yet the control levels are still unsatisfactory. This study investigated the antifungal activities of tea tree (Melaleuca alternifolia) essential oil (TTO) and hydrosol (TTH) against Foc TR4 and their bioactive components. The potential of TTO and TTH in inhibiting the growth of Foc TR4 was evaluated in vitro using agar well diffusion and spore germination assays. Compared to the chemical fungicide, TTO effectively suppressed the mycelial growth of Foc TR4 at 69%. Both the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of TTO and TTH were established at 0.2 µg/µL and 50% v/v, respectively, suggesting the fungicidal nature of the plant extracts. The disease control efficacies were also demonstrated by a (p ≤ 0.05) delayed Fusarium wilt symptom development in the susceptible banana plants with reduced LSI dan RDI scores from 70% to around 20–30%. A GC/MS analysis of TTO identified terpinen-4-ol, eucalyptol, and α-terpineol as the major components. In contrast, an LC/MS analysis of TTH identified different compounds, including dihydro-jasmonic acid and methyl ester. Our findings indicate the potential of tea tree extracts as natural alternatives to chemical fungicides to control Foc TR4. Multidisciplinary Digital Publishing Institute 2023-05-31 Article PeerReviewed Paramalingam, Pavitra and Baharum, Nadiya Akmal and Abdullah, Janna Ong and Hong, Jeum Kyu and Saidi, Noor Baity (2023) Antifungal potential of Melaleuca alternifolia against fungal pathogen Fusarium oxysporum f. sp. cubense Tropical Race 4. Molecules, 28 (11). art. no. 4456. pp. 1-12. ISSN 1420-3049 https://www.mdpi.com/1420-3049/28/11/4456 10.3390/molecules28114456
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description Fusarium wilt of bananas caused by Fusarium oxysporum f. sp. cubense Tropical Race 4 (Foc TR4) poses the most serious threat to banana production globally. The disease has been managed using chemical fungicides, yet the control levels are still unsatisfactory. This study investigated the antifungal activities of tea tree (Melaleuca alternifolia) essential oil (TTO) and hydrosol (TTH) against Foc TR4 and their bioactive components. The potential of TTO and TTH in inhibiting the growth of Foc TR4 was evaluated in vitro using agar well diffusion and spore germination assays. Compared to the chemical fungicide, TTO effectively suppressed the mycelial growth of Foc TR4 at 69%. Both the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of TTO and TTH were established at 0.2 µg/µL and 50% v/v, respectively, suggesting the fungicidal nature of the plant extracts. The disease control efficacies were also demonstrated by a (p ≤ 0.05) delayed Fusarium wilt symptom development in the susceptible banana plants with reduced LSI dan RDI scores from 70% to around 20–30%. A GC/MS analysis of TTO identified terpinen-4-ol, eucalyptol, and α-terpineol as the major components. In contrast, an LC/MS analysis of TTH identified different compounds, including dihydro-jasmonic acid and methyl ester. Our findings indicate the potential of tea tree extracts as natural alternatives to chemical fungicides to control Foc TR4.
format Article
author Paramalingam, Pavitra
Baharum, Nadiya Akmal
Abdullah, Janna Ong
Hong, Jeum Kyu
Saidi, Noor Baity
spellingShingle Paramalingam, Pavitra
Baharum, Nadiya Akmal
Abdullah, Janna Ong
Hong, Jeum Kyu
Saidi, Noor Baity
Antifungal potential of Melaleuca alternifolia against fungal pathogen Fusarium oxysporum f. sp. cubense Tropical Race 4
author_facet Paramalingam, Pavitra
Baharum, Nadiya Akmal
Abdullah, Janna Ong
Hong, Jeum Kyu
Saidi, Noor Baity
author_sort Paramalingam, Pavitra
title Antifungal potential of Melaleuca alternifolia against fungal pathogen Fusarium oxysporum f. sp. cubense Tropical Race 4
title_short Antifungal potential of Melaleuca alternifolia against fungal pathogen Fusarium oxysporum f. sp. cubense Tropical Race 4
title_full Antifungal potential of Melaleuca alternifolia against fungal pathogen Fusarium oxysporum f. sp. cubense Tropical Race 4
title_fullStr Antifungal potential of Melaleuca alternifolia against fungal pathogen Fusarium oxysporum f. sp. cubense Tropical Race 4
title_full_unstemmed Antifungal potential of Melaleuca alternifolia against fungal pathogen Fusarium oxysporum f. sp. cubense Tropical Race 4
title_sort antifungal potential of melaleuca alternifolia against fungal pathogen fusarium oxysporum f. sp. cubense tropical race 4
publisher Multidisciplinary Digital Publishing Institute
publishDate 2023
url http://psasir.upm.edu.my/id/eprint/109374/
https://www.mdpi.com/1420-3049/28/11/4456
_version_ 1806690497619558400
score 13.211869

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