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Preliminary detection method for heavy metals biomonitoring via inhibitive assay of brain acetylcholinesterase from Diodon hystrix

Acetylcholinesterase (AChE) is a well-known enzyme sensitive to pesticide exposure, but heavy metal sensitivity is rarely reported. Here, AChE extracted from the brain tissue of Diodon hystrix was exploited and used in a fast and economical way to sensitively detect the existence of heavy metals in...

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Main Authors: Nordin, Noreen, Abdulla, Rahmath, Tahir, Rohana, Jawan, Roslina, Mohd Hamdan, Diana Demiyah, Zahari, Nur Zaida, Gansau, Jualang Azlan, Shukor, Mohd Yunus, Sabullah, Mohd Khalizan
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Published: Universiti Malaysia Terengganu 2023
Online Access:http://psasir.upm.edu.my/id/eprint/109206/
https://jssm.umt.edu.my/wp-content/uploads/sites/51/2023/06/3-JSSM-Volume-18-Number-2-February-2023-FINAL.pdf
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spelling my.upm.eprints.1092062024-08-27T04:05:08Z http://psasir.upm.edu.my/id/eprint/109206/ Preliminary detection method for heavy metals biomonitoring via inhibitive assay of brain acetylcholinesterase from Diodon hystrix Nordin, Noreen Abdulla, Rahmath Tahir, Rohana Jawan, Roslina Mohd Hamdan, Diana Demiyah Zahari, Nur Zaida Gansau, Jualang Azlan Shukor, Mohd Yunus Sabullah, Mohd Khalizan Acetylcholinesterase (AChE) is a well-known enzyme sensitive to pesticide exposure, but heavy metal sensitivity is rarely reported. Here, AChE extracted from the brain tissue of Diodon hystrix was exploited and used in a fast and economical way to sensitively detect the existence of heavy metals in a water sample. An inhibitive enzyme assay was conducted, and the activity of AChE was found to be sensitive (> 20% inhibition) to sub-million levels of arsenic, cadmium, nickel, and zinc and less sensitive (< 20% inhibition) to copper and lead. While exposure to argentum, cobalt, and chromium shows no significant inhibition, AChE is not sensitive to those metal ions (p < 0.05). Field test work has proved that the assay is suitable for preliminary detecting heavy metal contamination in the river, especially near industrial and mining sites. Secondary validation was performed using ICP-OES to identify and measure the number of elements in the sample and compare them to the inhibition level of AChE activity. Universiti Malaysia Terengganu 2023-02 Article PeerReviewed Nordin, Noreen and Abdulla, Rahmath and Tahir, Rohana and Jawan, Roslina and Mohd Hamdan, Diana Demiyah and Zahari, Nur Zaida and Gansau, Jualang Azlan and Shukor, Mohd Yunus and Sabullah, Mohd Khalizan (2023) Preliminary detection method for heavy metals biomonitoring via inhibitive assay of brain acetylcholinesterase from Diodon hystrix. Journal of Sustainability Science and Management, 18 (2). pp. 24-33. ISSN 1823-8556; ESSN: 2672-7226 https://jssm.umt.edu.my/wp-content/uploads/sites/51/2023/06/3-JSSM-Volume-18-Number-2-February-2023-FINAL.pdf 10.46754/jssm.2023.02.003
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description Acetylcholinesterase (AChE) is a well-known enzyme sensitive to pesticide exposure, but heavy metal sensitivity is rarely reported. Here, AChE extracted from the brain tissue of Diodon hystrix was exploited and used in a fast and economical way to sensitively detect the existence of heavy metals in a water sample. An inhibitive enzyme assay was conducted, and the activity of AChE was found to be sensitive (> 20% inhibition) to sub-million levels of arsenic, cadmium, nickel, and zinc and less sensitive (< 20% inhibition) to copper and lead. While exposure to argentum, cobalt, and chromium shows no significant inhibition, AChE is not sensitive to those metal ions (p < 0.05). Field test work has proved that the assay is suitable for preliminary detecting heavy metal contamination in the river, especially near industrial and mining sites. Secondary validation was performed using ICP-OES to identify and measure the number of elements in the sample and compare them to the inhibition level of AChE activity.
format Article
author Nordin, Noreen
Abdulla, Rahmath
Tahir, Rohana
Jawan, Roslina
Mohd Hamdan, Diana Demiyah
Zahari, Nur Zaida
Gansau, Jualang Azlan
Shukor, Mohd Yunus
Sabullah, Mohd Khalizan
spellingShingle Nordin, Noreen
Abdulla, Rahmath
Tahir, Rohana
Jawan, Roslina
Mohd Hamdan, Diana Demiyah
Zahari, Nur Zaida
Gansau, Jualang Azlan
Shukor, Mohd Yunus
Sabullah, Mohd Khalizan
Preliminary detection method for heavy metals biomonitoring via inhibitive assay of brain acetylcholinesterase from Diodon hystrix
author_facet Nordin, Noreen
Abdulla, Rahmath
Tahir, Rohana
Jawan, Roslina
Mohd Hamdan, Diana Demiyah
Zahari, Nur Zaida
Gansau, Jualang Azlan
Shukor, Mohd Yunus
Sabullah, Mohd Khalizan
author_sort Nordin, Noreen
title Preliminary detection method for heavy metals biomonitoring via inhibitive assay of brain acetylcholinesterase from Diodon hystrix
title_short Preliminary detection method for heavy metals biomonitoring via inhibitive assay of brain acetylcholinesterase from Diodon hystrix
title_full Preliminary detection method for heavy metals biomonitoring via inhibitive assay of brain acetylcholinesterase from Diodon hystrix
title_fullStr Preliminary detection method for heavy metals biomonitoring via inhibitive assay of brain acetylcholinesterase from Diodon hystrix
title_full_unstemmed Preliminary detection method for heavy metals biomonitoring via inhibitive assay of brain acetylcholinesterase from Diodon hystrix
title_sort preliminary detection method for heavy metals biomonitoring via inhibitive assay of brain acetylcholinesterase from diodon hystrix
publisher Universiti Malaysia Terengganu
publishDate 2023
url http://psasir.upm.edu.my/id/eprint/109206/
https://jssm.umt.edu.my/wp-content/uploads/sites/51/2023/06/3-JSSM-Volume-18-Number-2-February-2023-FINAL.pdf
_version_ 1809142975768297472
score 13.244404

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