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Induced expression of Ganoderma boninense lanosterol 14α-demethylase (ERG11) during interaction with oil palm

Background: The basidiomycete fungus, Ganoderma boninense is the main contributor to oil palm Basal Stem Rot (BSR) in Malaysia and Indonesia. Lanosterol 14α-Demethylase (ERG11) is a key enzyme involved in biosynthesis of ergosterol, which is an important component in the fungal cell membrane. The Az...

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Main Authors: Lim, Fook-Hwa, Abd Rasid, Omar, Abu Seman, Idris, Abdul Wahab, Mohd As’wad, Vadamalai, Ganesan, Ahmad Parveez, Ghulam Kadir, Wong, Mui-Yun
Format: Article
Published: Springer 2023
Online Access:http://psasir.upm.edu.my/id/eprint/109079/
https://link.springer.com/article/10.1007/s11033-022-08131-4?error=cookies_not_supported&code=d378445c-0744-44b8-98e1-66a10cf9f78c
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spelling my.upm.eprints.1090792024-09-02T06:49:01Z http://psasir.upm.edu.my/id/eprint/109079/ Induced expression of Ganoderma boninense lanosterol 14α-demethylase (ERG11) during interaction with oil palm Lim, Fook-Hwa Abd Rasid, Omar Abu Seman, Idris Abdul Wahab, Mohd As’wad Vadamalai, Ganesan Ahmad Parveez, Ghulam Kadir Wong, Mui-Yun Background: The basidiomycete fungus, Ganoderma boninense is the main contributor to oil palm Basal Stem Rot (BSR) in Malaysia and Indonesia. Lanosterol 14α-Demethylase (ERG11) is a key enzyme involved in biosynthesis of ergosterol, which is an important component in the fungal cell membrane. The Azole group fungicides are effective against pathogenic fungi including G. boninense by inhibiting the ERG11 activity. However, the work on molecular characterization of G. boninense ERG11 is still unavailable today. Methods and results: This study aimed to isolate and characterize the full-length cDNA encoding ERG11 from G. boninense. The G. boninense ERG11 gene expression during interaction with oil palm was also studied. A full-length 1860 bp cDNA encoding ERG11 was successfully isolated from G. boninense. The G. boninense ERG11 shared 91% similarity to ERG11 from other basidiomycete fungi. The protein structure homology modeling of GbERG11 was analyzed using the SWISS-MODEL workspace. Southern blot and genome data analyses showed that there is only a single copy of ERG11 gene in the G. boninense genome. Based on the in-vitro inoculation study, the ERG11 gene expression in G. boninense has shown almost 2-fold upregulation with the presence of oil palm. Conclusion: This study provided molecular information and characterization study on the G. boninense ERG11 and this knowledge could be used to design effective control measures to tackle the BSR disease of oil palm. Springer 2023-03 Article PeerReviewed Lim, Fook-Hwa and Abd Rasid, Omar and Abu Seman, Idris and Abdul Wahab, Mohd As’wad and Vadamalai, Ganesan and Ahmad Parveez, Ghulam Kadir and Wong, Mui-Yun (2023) Induced expression of Ganoderma boninense lanosterol 14α-demethylase (ERG11) during interaction with oil palm. Molecular Biology Reports, 50 (3). pp. 2367-2379. ISSN 0301-4851; ESSN: 1573-4978 https://link.springer.com/article/10.1007/s11033-022-08131-4?error=cookies_not_supported&code=d378445c-0744-44b8-98e1-66a10cf9f78c 10.1007/s11033-022-08131-4
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description Background: The basidiomycete fungus, Ganoderma boninense is the main contributor to oil palm Basal Stem Rot (BSR) in Malaysia and Indonesia. Lanosterol 14α-Demethylase (ERG11) is a key enzyme involved in biosynthesis of ergosterol, which is an important component in the fungal cell membrane. The Azole group fungicides are effective against pathogenic fungi including G. boninense by inhibiting the ERG11 activity. However, the work on molecular characterization of G. boninense ERG11 is still unavailable today. Methods and results: This study aimed to isolate and characterize the full-length cDNA encoding ERG11 from G. boninense. The G. boninense ERG11 gene expression during interaction with oil palm was also studied. A full-length 1860 bp cDNA encoding ERG11 was successfully isolated from G. boninense. The G. boninense ERG11 shared 91% similarity to ERG11 from other basidiomycete fungi. The protein structure homology modeling of GbERG11 was analyzed using the SWISS-MODEL workspace. Southern blot and genome data analyses showed that there is only a single copy of ERG11 gene in the G. boninense genome. Based on the in-vitro inoculation study, the ERG11 gene expression in G. boninense has shown almost 2-fold upregulation with the presence of oil palm. Conclusion: This study provided molecular information and characterization study on the G. boninense ERG11 and this knowledge could be used to design effective control measures to tackle the BSR disease of oil palm.
format Article
author Lim, Fook-Hwa
Abd Rasid, Omar
Abu Seman, Idris
Abdul Wahab, Mohd As’wad
Vadamalai, Ganesan
Ahmad Parveez, Ghulam Kadir
Wong, Mui-Yun
spellingShingle Lim, Fook-Hwa
Abd Rasid, Omar
Abu Seman, Idris
Abdul Wahab, Mohd As’wad
Vadamalai, Ganesan
Ahmad Parveez, Ghulam Kadir
Wong, Mui-Yun
Induced expression of Ganoderma boninense lanosterol 14α-demethylase (ERG11) during interaction with oil palm
author_facet Lim, Fook-Hwa
Abd Rasid, Omar
Abu Seman, Idris
Abdul Wahab, Mohd As’wad
Vadamalai, Ganesan
Ahmad Parveez, Ghulam Kadir
Wong, Mui-Yun
author_sort Lim, Fook-Hwa
title Induced expression of Ganoderma boninense lanosterol 14α-demethylase (ERG11) during interaction with oil palm
title_short Induced expression of Ganoderma boninense lanosterol 14α-demethylase (ERG11) during interaction with oil palm
title_full Induced expression of Ganoderma boninense lanosterol 14α-demethylase (ERG11) during interaction with oil palm
title_fullStr Induced expression of Ganoderma boninense lanosterol 14α-demethylase (ERG11) during interaction with oil palm
title_full_unstemmed Induced expression of Ganoderma boninense lanosterol 14α-demethylase (ERG11) during interaction with oil palm
title_sort induced expression of ganoderma boninense lanosterol 14α-demethylase (erg11) during interaction with oil palm
publisher Springer
publishDate 2023
url http://psasir.upm.edu.my/id/eprint/109079/
https://link.springer.com/article/10.1007/s11033-022-08131-4?error=cookies_not_supported&code=d378445c-0744-44b8-98e1-66a10cf9f78c
_version_ 1811686032673865728
score 13.211869

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