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Prevalence and diversity of antibiotic resistance heterotrophic bacteria found along the Bintulu Rivers, Sarawak

The study aims (1) to isolate and characterise the heterotrophic bacteria from different rivers in Bintulu, Sarawak, (2) to investigate the hygienic condition of the rivers through faecal coliforms and (3) to determine the antibiotic resistance among the heterotrophic bacteria isolates. A total of...

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Main Authors: Leong, Sui Sien, Hamli, Hadi, Abdul Rahim, Erra Fazira, Mustafa, Suhaili
Format: Article
Published: UNIMAS Publisher 2023
Online Access:http://psasir.upm.edu.my/id/eprint/109063/
https://publisher.unimas.my/ojs/index.php/BJRST/article/view/5450
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spelling my.upm.eprints.1090632024-10-14T03:56:48Z http://psasir.upm.edu.my/id/eprint/109063/ Prevalence and diversity of antibiotic resistance heterotrophic bacteria found along the Bintulu Rivers, Sarawak Leong, Sui Sien Hamli, Hadi Abdul Rahim, Erra Fazira Mustafa, Suhaili The study aims (1) to isolate and characterise the heterotrophic bacteria from different rivers in Bintulu, Sarawak, (2) to investigate the hygienic condition of the rivers through faecal coliforms and (3) to determine the antibiotic resistance among the heterotrophic bacteria isolates. A total of 100 heterotrophic bacteria strains were identified from rivers of Bintulu, Sarawak. The characterisation of bacteria was performed using (GTG)5 fingerprinting to investigate their genetic distribution diversity and 16S rRNA gene sequencing. Microbiological variables tested including total viable count, coliform count, Escherichia coli confirmation test. Antibiotic susceptibility test was performed against 10 antibiotics. Sample collected from Sungai Teknik showed higher mean bacterial population size (log 4.48 ± 0.00 CFU/mL). Sungai Sibiu, Waterfront and Sungai Plan revealed the highest most probable number (MPN) index (>1,600 per mL). Each dendrogram showed 3 to 7 clusters of bacteria groups confirmed as Enterobacter spp. (40%), Acinetobacter spp. (13%), Bacillus spp. (13%), Klebsiella spp. (13%), Staphylococcus spp. (7%), Chromobacterium spp. (7%) and Citrobacter spp. (7%). All the heterotrophic bacteria isolated showed high resistance against ciprofloxacin (63.70 ± 33.40%), piperacillin (58.10 ± 31.37%), aztreonam (48.40 ± 30.95%) and more susceptible to tetracycline (3.30 ± 10.44%). Our findings highlight the multiple antibiotics resistance and microbiological analysis of heterotrophic bacteria found in polluted river water. The preservation of the river water is vital as hydrologic purposes, sustain the microbial composition, ecological integrity of the river. UNIMAS Publisher 2023-12-24 Article PeerReviewed Leong, Sui Sien and Hamli, Hadi and Abdul Rahim, Erra Fazira and Mustafa, Suhaili (2023) Prevalence and diversity of antibiotic resistance heterotrophic bacteria found along the Bintulu Rivers, Sarawak. Borneo Journal of Resource Science and Technology, 13 (2). pp. 32-41. ISSN 2229-9769; ESSN: 0128-2972 https://publisher.unimas.my/ojs/index.php/BJRST/article/view/5450 10.33736/bjrst.5450.2023
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description The study aims (1) to isolate and characterise the heterotrophic bacteria from different rivers in Bintulu, Sarawak, (2) to investigate the hygienic condition of the rivers through faecal coliforms and (3) to determine the antibiotic resistance among the heterotrophic bacteria isolates. A total of 100 heterotrophic bacteria strains were identified from rivers of Bintulu, Sarawak. The characterisation of bacteria was performed using (GTG)5 fingerprinting to investigate their genetic distribution diversity and 16S rRNA gene sequencing. Microbiological variables tested including total viable count, coliform count, Escherichia coli confirmation test. Antibiotic susceptibility test was performed against 10 antibiotics. Sample collected from Sungai Teknik showed higher mean bacterial population size (log 4.48 ± 0.00 CFU/mL). Sungai Sibiu, Waterfront and Sungai Plan revealed the highest most probable number (MPN) index (>1,600 per mL). Each dendrogram showed 3 to 7 clusters of bacteria groups confirmed as Enterobacter spp. (40%), Acinetobacter spp. (13%), Bacillus spp. (13%), Klebsiella spp. (13%), Staphylococcus spp. (7%), Chromobacterium spp. (7%) and Citrobacter spp. (7%). All the heterotrophic bacteria isolated showed high resistance against ciprofloxacin (63.70 ± 33.40%), piperacillin (58.10 ± 31.37%), aztreonam (48.40 ± 30.95%) and more susceptible to tetracycline (3.30 ± 10.44%). Our findings highlight the multiple antibiotics resistance and microbiological analysis of heterotrophic bacteria found in polluted river water. The preservation of the river water is vital as hydrologic purposes, sustain the microbial composition, ecological integrity of the river.
format Article
author Leong, Sui Sien
Hamli, Hadi
Abdul Rahim, Erra Fazira
Mustafa, Suhaili
spellingShingle Leong, Sui Sien
Hamli, Hadi
Abdul Rahim, Erra Fazira
Mustafa, Suhaili
Prevalence and diversity of antibiotic resistance heterotrophic bacteria found along the Bintulu Rivers, Sarawak
author_facet Leong, Sui Sien
Hamli, Hadi
Abdul Rahim, Erra Fazira
Mustafa, Suhaili
author_sort Leong, Sui Sien
title Prevalence and diversity of antibiotic resistance heterotrophic bacteria found along the Bintulu Rivers, Sarawak
title_short Prevalence and diversity of antibiotic resistance heterotrophic bacteria found along the Bintulu Rivers, Sarawak
title_full Prevalence and diversity of antibiotic resistance heterotrophic bacteria found along the Bintulu Rivers, Sarawak
title_fullStr Prevalence and diversity of antibiotic resistance heterotrophic bacteria found along the Bintulu Rivers, Sarawak
title_full_unstemmed Prevalence and diversity of antibiotic resistance heterotrophic bacteria found along the Bintulu Rivers, Sarawak
title_sort prevalence and diversity of antibiotic resistance heterotrophic bacteria found along the bintulu rivers, sarawak
publisher UNIMAS Publisher
publishDate 2023
url http://psasir.upm.edu.my/id/eprint/109063/
https://publisher.unimas.my/ojs/index.php/BJRST/article/view/5450
_version_ 1814054687963348992
score 13.211869

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