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The indigenous microbial diversity involved in the spontaneous fermentation of red dragon fruit (Hylocereus polyrhizus) identified by means of molecular tools

Red dragon fruit (RDF) is well-known for its high nutritional content, especially the red pigment betacyanins that possess high antioxidant activity. Natural fermentation is an ancient yet outstanding technique that relies on the autochthonous microbiota from fruits and vegetables surfaces to preser...

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Main Authors: Lim, Teck Wei, Choo, Kah Yee, Lim, Renee Lay Hong, Pui, Liew Phing, Tan, Chin Ping, Ho, Chun Wai
Format: Article
Language:English
Published: Elsevier 2023
Online Access:http://psasir.upm.edu.my/id/eprint/108760/1/The%20indigenous%20microbial%20diversity.pdf
http://psasir.upm.edu.my/id/eprint/108760/
https://www.cell.com/heliyon/fulltext/S2405-8440(23)09148-X?
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spelling my.upm.eprints.1087602024-10-10T07:36:45Z http://psasir.upm.edu.my/id/eprint/108760/ The indigenous microbial diversity involved in the spontaneous fermentation of red dragon fruit (Hylocereus polyrhizus) identified by means of molecular tools Lim, Teck Wei Choo, Kah Yee Lim, Renee Lay Hong Pui, Liew Phing Tan, Chin Ping Ho, Chun Wai Red dragon fruit (RDF) is well-known for its high nutritional content, especially the red pigment betacyanins that possess high antioxidant activity. Natural fermentation is an ancient yet outstanding technique that relies on the autochthonous microbiota from fruits and vegetables surfaces to preserve and improve the nutritional values and quality of the food product. The present study was to evaluate and identify the indigenous microbial community (bacteria and fungi) that are involved in the natural fermentation of RDF. Results revealed a total of twenty bacterial pure cultures and nine fungal pure cultures were successfully isolated from fermented red dragon fruit drink (FRDFD). For the first time, the PCR amplification of 16S rRNA and ITS regions and sequence analysis suggested nine genera of bacteria and three genera of fungi (Aureobasidium pullulans, Clavispora opuntiae, and Talaromyces aurantiacus) present in the FRDFD. Four dominant (≥10 % isolates) bacteria species identified from FRDFD were Klebsiella pneumonia, Brevibacillus parabrevis, Bacillus tequilensis and Bacillus subtilis. The carbohydrate fermentation test showed that all the indigenous microbes identified were able to serve as useful starter culture by fermenting sucrose and glucose, thereby producing acid to lower the pH of FRDFD to around pH 4 for better betacyanins stability. The present study provides a more comprehensive understanding of the indigenous microbial community that serves as the starter culture in the fermentation of RDF. Besides, this study provides a useful guide for future research to be conducted on studying the rare bacterial strains (such as B. tequilensis) identified from the FRDFD for their potential bioactivities and applications in medical treatment and functional foods industries. Elsevier 2023-11-06 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/108760/1/The%20indigenous%20microbial%20diversity.pdf Lim, Teck Wei and Choo, Kah Yee and Lim, Renee Lay Hong and Pui, Liew Phing and Tan, Chin Ping and Ho, Chun Wai (2023) The indigenous microbial diversity involved in the spontaneous fermentation of red dragon fruit (Hylocereus polyrhizus) identified by means of molecular tools. Heliyon, 9 (11). art. no. e21940. pp. 1-10. ISSN 2405-8440 https://www.cell.com/heliyon/fulltext/S2405-8440(23)09148-X? 10.1016/j.heliyon.2023.e21940
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Red dragon fruit (RDF) is well-known for its high nutritional content, especially the red pigment betacyanins that possess high antioxidant activity. Natural fermentation is an ancient yet outstanding technique that relies on the autochthonous microbiota from fruits and vegetables surfaces to preserve and improve the nutritional values and quality of the food product. The present study was to evaluate and identify the indigenous microbial community (bacteria and fungi) that are involved in the natural fermentation of RDF. Results revealed a total of twenty bacterial pure cultures and nine fungal pure cultures were successfully isolated from fermented red dragon fruit drink (FRDFD). For the first time, the PCR amplification of 16S rRNA and ITS regions and sequence analysis suggested nine genera of bacteria and three genera of fungi (Aureobasidium pullulans, Clavispora opuntiae, and Talaromyces aurantiacus) present in the FRDFD. Four dominant (≥10 % isolates) bacteria species identified from FRDFD were Klebsiella pneumonia, Brevibacillus parabrevis, Bacillus tequilensis and Bacillus subtilis. The carbohydrate fermentation test showed that all the indigenous microbes identified were able to serve as useful starter culture by fermenting sucrose and glucose, thereby producing acid to lower the pH of FRDFD to around pH 4 for better betacyanins stability. The present study provides a more comprehensive understanding of the indigenous microbial community that serves as the starter culture in the fermentation of RDF. Besides, this study provides a useful guide for future research to be conducted on studying the rare bacterial strains (such as B. tequilensis) identified from the FRDFD for their potential bioactivities and applications in medical treatment and functional foods industries.
format Article
author Lim, Teck Wei
Choo, Kah Yee
Lim, Renee Lay Hong
Pui, Liew Phing
Tan, Chin Ping
Ho, Chun Wai
spellingShingle Lim, Teck Wei
Choo, Kah Yee
Lim, Renee Lay Hong
Pui, Liew Phing
Tan, Chin Ping
Ho, Chun Wai
The indigenous microbial diversity involved in the spontaneous fermentation of red dragon fruit (Hylocereus polyrhizus) identified by means of molecular tools
author_facet Lim, Teck Wei
Choo, Kah Yee
Lim, Renee Lay Hong
Pui, Liew Phing
Tan, Chin Ping
Ho, Chun Wai
author_sort Lim, Teck Wei
title The indigenous microbial diversity involved in the spontaneous fermentation of red dragon fruit (Hylocereus polyrhizus) identified by means of molecular tools
title_short The indigenous microbial diversity involved in the spontaneous fermentation of red dragon fruit (Hylocereus polyrhizus) identified by means of molecular tools
title_full The indigenous microbial diversity involved in the spontaneous fermentation of red dragon fruit (Hylocereus polyrhizus) identified by means of molecular tools
title_fullStr The indigenous microbial diversity involved in the spontaneous fermentation of red dragon fruit (Hylocereus polyrhizus) identified by means of molecular tools
title_full_unstemmed The indigenous microbial diversity involved in the spontaneous fermentation of red dragon fruit (Hylocereus polyrhizus) identified by means of molecular tools
title_sort indigenous microbial diversity involved in the spontaneous fermentation of red dragon fruit (hylocereus polyrhizus) identified by means of molecular tools
publisher Elsevier
publishDate 2023
url http://psasir.upm.edu.my/id/eprint/108760/1/The%20indigenous%20microbial%20diversity.pdf
http://psasir.upm.edu.my/id/eprint/108760/
https://www.cell.com/heliyon/fulltext/S2405-8440(23)09148-X?
_version_ 1814054666600710144
score 13.211869

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