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Optimization and elucidation of interactions between ammonium, nitrate and phosphate in Centella asiatica cell culture using response surface methodology

The effects of macronutrients (NO3−, NH4+ and PO43−) on cell growth and triterpenoids production inCentella asiatica cell suspension cultures were analyzed using the Box-Behnken response surface model experimental design. In screening and optimization experiments, PO43− as a single factor significan...

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Main Authors: Omar, Rozita, Abdullah, M. A., Hasan, M. A., Mahmood, Maziah, Mustapa Kamal, Siti Mazlina
Format: Article
Language:English
Published: Springer 2005
Online Access:http://psasir.upm.edu.my/id/eprint/10826/
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spelling my.upm.eprints.108262011-10-30T05:13:32Z http://psasir.upm.edu.my/id/eprint/10826/ Optimization and elucidation of interactions between ammonium, nitrate and phosphate in Centella asiatica cell culture using response surface methodology Omar, Rozita Abdullah, M. A. Hasan, M. A. Mahmood, Maziah Mustapa Kamal, Siti Mazlina The effects of macronutrients (NO3−, NH4+ and PO43−) on cell growth and triterpenoids production inCentella asiatica cell suspension cultures were analyzed using the Box-Behnken response surface model experimental design. In screening and optimization experiments, PO43− as a single factor significantly influenced cell growth where increasing the phosphate level from 0.1 to 2.4 or 2.6 mM, elevated cell growth from 3.9 to 14-16 g/L. The optimum values predicted from the response surface model are 5.05 mM NH4+, 15.0 mM NO3− and 2.6 mM PO43−, yielding 16.0 g/L cell dry weight with 99% fitness to the experimental data. While the NH4+-NO3− interaction influenced cell growth positively in the optimization experiment, NH4+ and NO3− as single factors; and interactions of NO3−-PO43−, NH4+-PO43− and NH4+-NO3− were all negative in the screening experiment. Cell growth and the final pH level were positively affected by PO43−, but negatively affected by NH4+ and NH4+-PO43− interactions. The different effects of factors and their interactions on cell growth and final pH are influenced by a broad or narrow range of macronutrient concentrations. The productions of triterpenoids however were lower than 4 mg/g cell dry weight. Springer 2005 Article PeerReviewed Omar, Rozita and Abdullah, M. A. and Hasan, M. A. and Mahmood, Maziah and Mustapa Kamal, Siti Mazlina (2005) Optimization and elucidation of interactions between ammonium, nitrate and phosphate in Centella asiatica cell culture using response surface methodology. Biotechnology and Bioprocess Engineering, 10 (3). pp. 192-197. ISSN 1226-8372 10.1007/BF02932012 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The effects of macronutrients (NO3−, NH4+ and PO43−) on cell growth and triterpenoids production inCentella asiatica cell suspension cultures were analyzed using the Box-Behnken response surface model experimental design. In screening and optimization experiments, PO43− as a single factor significantly influenced cell growth where increasing the phosphate level from 0.1 to 2.4 or 2.6 mM, elevated cell growth from 3.9 to 14-16 g/L. The optimum values predicted from the response surface model are 5.05 mM NH4+, 15.0 mM NO3− and 2.6 mM PO43−, yielding 16.0 g/L cell dry weight with 99% fitness to the experimental data. While the NH4+-NO3− interaction influenced cell growth positively in the optimization experiment, NH4+ and NO3− as single factors; and interactions of NO3−-PO43−, NH4+-PO43− and NH4+-NO3− were all negative in the screening experiment. Cell growth and the final pH level were positively affected by PO43−, but negatively affected by NH4+ and NH4+-PO43− interactions. The different effects of factors and their interactions on cell growth and final pH are influenced by a broad or narrow range of macronutrient concentrations. The productions of triterpenoids however were lower than 4 mg/g cell dry weight.
format Article
author Omar, Rozita
Abdullah, M. A.
Hasan, M. A.
Mahmood, Maziah
Mustapa Kamal, Siti Mazlina
spellingShingle Omar, Rozita
Abdullah, M. A.
Hasan, M. A.
Mahmood, Maziah
Mustapa Kamal, Siti Mazlina
Optimization and elucidation of interactions between ammonium, nitrate and phosphate in Centella asiatica cell culture using response surface methodology
author_facet Omar, Rozita
Abdullah, M. A.
Hasan, M. A.
Mahmood, Maziah
Mustapa Kamal, Siti Mazlina
author_sort Omar, Rozita
title Optimization and elucidation of interactions between ammonium, nitrate and phosphate in Centella asiatica cell culture using response surface methodology
title_short Optimization and elucidation of interactions between ammonium, nitrate and phosphate in Centella asiatica cell culture using response surface methodology
title_full Optimization and elucidation of interactions between ammonium, nitrate and phosphate in Centella asiatica cell culture using response surface methodology
title_fullStr Optimization and elucidation of interactions between ammonium, nitrate and phosphate in Centella asiatica cell culture using response surface methodology
title_full_unstemmed Optimization and elucidation of interactions between ammonium, nitrate and phosphate in Centella asiatica cell culture using response surface methodology
title_sort optimization and elucidation of interactions between ammonium, nitrate and phosphate in centella asiatica cell culture using response surface methodology
publisher Springer
publishDate 2005
url http://psasir.upm.edu.my/id/eprint/10826/
_version_ 1643824698548551680
score 13.244404

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