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Identification of the oil palm bagworm, Mahasena corbetti tams (Lepidoptera: Psychidae) via molecular techniques and its biocontrol assay using Bacillus thuringiensis

The larvae of the bagworm Mahasena corbetti Tams (Lepidoptera: Psychidae) cause significant yield losses by defoliation of the oil palm crop in Malaysia. This necessitates the development of an effective and sustainable control strategies against this pest. The objective of this study was to identif...

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Main Authors: Thakib Maidin, Mohd Shawal, Mohd Hashim, Amalia, Kamarudin, Noorhazwani, Ahmad, Siti Nurulhidayah, Mohd. Masri, Mohamed Mazmira, Abdul Rahim, Raha, Jamian, Syari
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Published: Malaysian Palm Oil Board 2023
Online Access:http://psasir.upm.edu.my/id/eprint/108251/
http://jopr.mpob.gov.my/identification-of-the-oil-palm-bagworm-imahasena-corbettii-tams-lepidoptera-psychidae-via-molecular-techniques-and-its-biocontrol-assay-using-ibacillus-thuringiensisi/
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spelling my.upm.eprints.1082512024-09-23T01:57:14Z http://psasir.upm.edu.my/id/eprint/108251/ Identification of the oil palm bagworm, Mahasena corbetti tams (Lepidoptera: Psychidae) via molecular techniques and its biocontrol assay using Bacillus thuringiensis Thakib Maidin, Mohd Shawal Mohd Hashim, Amalia Kamarudin, Noorhazwani Ahmad, Siti Nurulhidayah Mohd. Masri, Mohamed Mazmira Abdul Rahim, Raha Jamian, Syari The larvae of the bagworm Mahasena corbetti Tams (Lepidoptera: Psychidae) cause significant yield losses by defoliation of the oil palm crop in Malaysia. This necessitates the development of an effective and sustainable control strategies against this pest. The objective of this study was to identify M. corbetti via molecular techniques and evaluate the potential of a local strain of Bacillus thuringiensis (MPOB Bt1), as a biocontrol agent for the pest. Morphological and molecular analysis was performed to identify M. corbetti, using the mitochondrial cytochrome oxidase I (COI) gene for genetic identification. The results showed that M. corbetti isolate SH01 (GenBank accession number MN373269) had a high degree of similarity (98.52%, 0.0 E-value) with the COI gene of M. corbetti voucher GB270DN1795 (GenBank accession number MG574312) collected in India and 100% BOLD similarities with the COI gene of M. corbetti from Singapore (BOLD ID PSYCH113-12). Laboratory bioassay showed 100% mortality of M. corbetti larvae three days after treatment with MPOB Bt1 (108 spores/mL). The results demonstrate the potential of MPOB Bt1 as an environmentally friendly biopesticide against M. corbetti in oil palm, but optimisation and efficacy in other pests require further research. Furthermore, biocontrol can be used as part of an integrated pest management program against bagworms and other lepidopteran pests. Malaysian Palm Oil Board 2023 Article PeerReviewed Thakib Maidin, Mohd Shawal and Mohd Hashim, Amalia and Kamarudin, Noorhazwani and Ahmad, Siti Nurulhidayah and Mohd. Masri, Mohamed Mazmira and Abdul Rahim, Raha and Jamian, Syari (2023) Identification of the oil palm bagworm, Mahasena corbetti tams (Lepidoptera: Psychidae) via molecular techniques and its biocontrol assay using Bacillus thuringiensis. Journal of Oil Palm Research. pp. 1-13. ISSN 2811-4701 http://jopr.mpob.gov.my/identification-of-the-oil-palm-bagworm-imahasena-corbettii-tams-lepidoptera-psychidae-via-molecular-techniques-and-its-biocontrol-assay-using-ibacillus-thuringiensisi/ 10.21894/jopr.2023.0036
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description The larvae of the bagworm Mahasena corbetti Tams (Lepidoptera: Psychidae) cause significant yield losses by defoliation of the oil palm crop in Malaysia. This necessitates the development of an effective and sustainable control strategies against this pest. The objective of this study was to identify M. corbetti via molecular techniques and evaluate the potential of a local strain of Bacillus thuringiensis (MPOB Bt1), as a biocontrol agent for the pest. Morphological and molecular analysis was performed to identify M. corbetti, using the mitochondrial cytochrome oxidase I (COI) gene for genetic identification. The results showed that M. corbetti isolate SH01 (GenBank accession number MN373269) had a high degree of similarity (98.52%, 0.0 E-value) with the COI gene of M. corbetti voucher GB270DN1795 (GenBank accession number MG574312) collected in India and 100% BOLD similarities with the COI gene of M. corbetti from Singapore (BOLD ID PSYCH113-12). Laboratory bioassay showed 100% mortality of M. corbetti larvae three days after treatment with MPOB Bt1 (108 spores/mL). The results demonstrate the potential of MPOB Bt1 as an environmentally friendly biopesticide against M. corbetti in oil palm, but optimisation and efficacy in other pests require further research. Furthermore, biocontrol can be used as part of an integrated pest management program against bagworms and other lepidopteran pests.
format Article
author Thakib Maidin, Mohd Shawal
Mohd Hashim, Amalia
Kamarudin, Noorhazwani
Ahmad, Siti Nurulhidayah
Mohd. Masri, Mohamed Mazmira
Abdul Rahim, Raha
Jamian, Syari
spellingShingle Thakib Maidin, Mohd Shawal
Mohd Hashim, Amalia
Kamarudin, Noorhazwani
Ahmad, Siti Nurulhidayah
Mohd. Masri, Mohamed Mazmira
Abdul Rahim, Raha
Jamian, Syari
Identification of the oil palm bagworm, Mahasena corbetti tams (Lepidoptera: Psychidae) via molecular techniques and its biocontrol assay using Bacillus thuringiensis
author_facet Thakib Maidin, Mohd Shawal
Mohd Hashim, Amalia
Kamarudin, Noorhazwani
Ahmad, Siti Nurulhidayah
Mohd. Masri, Mohamed Mazmira
Abdul Rahim, Raha
Jamian, Syari
author_sort Thakib Maidin, Mohd Shawal
title Identification of the oil palm bagworm, Mahasena corbetti tams (Lepidoptera: Psychidae) via molecular techniques and its biocontrol assay using Bacillus thuringiensis
title_short Identification of the oil palm bagworm, Mahasena corbetti tams (Lepidoptera: Psychidae) via molecular techniques and its biocontrol assay using Bacillus thuringiensis
title_full Identification of the oil palm bagworm, Mahasena corbetti tams (Lepidoptera: Psychidae) via molecular techniques and its biocontrol assay using Bacillus thuringiensis
title_fullStr Identification of the oil palm bagworm, Mahasena corbetti tams (Lepidoptera: Psychidae) via molecular techniques and its biocontrol assay using Bacillus thuringiensis
title_full_unstemmed Identification of the oil palm bagworm, Mahasena corbetti tams (Lepidoptera: Psychidae) via molecular techniques and its biocontrol assay using Bacillus thuringiensis
title_sort identification of the oil palm bagworm, mahasena corbetti tams (lepidoptera: psychidae) via molecular techniques and its biocontrol assay using bacillus thuringiensis
publisher Malaysian Palm Oil Board
publishDate 2023
url http://psasir.upm.edu.my/id/eprint/108251/
http://jopr.mpob.gov.my/identification-of-the-oil-palm-bagworm-imahasena-corbettii-tams-lepidoptera-psychidae-via-molecular-techniques-and-its-biocontrol-assay-using-ibacillus-thuringiensisi/
_version_ 1811686013459759104
score 13.211869

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