Staff View: A single targeted gamma-ray irradiation induced an acute modulation of immune cells and related cytokines in EMT6 mouse-bearing tumour model

A single targeted gamma-ray irradiation induced an acute modulation of immune cells and related cytokines in EMT6 mouse-bearing tumour model

BACKGROUND: A complicated interplay between radiation doses, tumour microenvironment (TME), and host immune system is linked to the active participation of immune response. OBJECTIVE: The effects of single targeted 2 Gy and 8 Gy gamma-ray irradiations on the immune cell population (lymphocytes, B-ce...

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Main Authors:	Hasan, Nurhaslina, Hasani, Narimah Abdul Hamid, Omar, Effat, Sham, Fatihah Ronny, Fuad, Syed Baharom Syed Ahmad, Abdul Karim, Muhammad Khalis, Ibahim, Mohammad Johari
Format:	Article
Published:	IOS Press 2023
Online Access:	http://psasir.upm.edu.my/id/eprint/106714/ https://content.iospress.com/articles/cancer-biomarkers/cbm220268
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spelling	my.upm.eprints.1067142024-09-26T07:46:43Z http://psasir.upm.edu.my/id/eprint/106714/ A single targeted gamma-ray irradiation induced an acute modulation of immune cells and related cytokines in EMT6 mouse-bearing tumour model Hasan, Nurhaslina Hasani, Narimah Abdul Hamid Omar, Effat Sham, Fatihah Ronny Fuad, Syed Baharom Syed Ahmad Abdul Karim, Muhammad Khalis Ibahim, Mohammad Johari BACKGROUND: A complicated interplay between radiation doses, tumour microenvironment (TME), and host immune system is linked to the active participation of immune response. OBJECTIVE: The effects of single targeted 2 Gy and 8 Gy gamma-ray irradiations on the immune cell population (lymphocytes, B-cells, T-cells, neutrophils, eosinophils, and macrophages) in EMT6 mouse-bearing tumour models was investigated. METHODS: The effects of both irradiation doses in early (96 hours) and acute phase (5 to 11 days) post-irradiation on immune parameters were monitored in blood circulation and TME using flow cytometry. Simultaneously, selected cytokines related to immune cells within the TME were measured using multiplex ELISA. RESULTS: A temporary reduction in systemic total white blood count (TWBC) resulted from an early phase (96 hours) of gamma-ray irradiation at 2 Gy and 8 Gy compared to sham control group. No difference was obtained in the acute phase. Neutrophils dominated among other immune cells in TME in sham control group. Eosinophils in TME was significantly increased after 8 Gy treatment in acute phase compared to sham control (p< 0.005). Furthermore, the increment of tumour necrosis (TNF)-±, eotaxin and interleukin (IL)-7 (p< 0.05) in both treatment groups and phases were associated with anti-tumour activities within TME by gamma-ray irradiation. CONCLUSION: The temporary changes in immune cell populations within systemic circulation and TME induced by different doses of gamma-ray irradiation correlated with suppression of several pro-tumorigenic cytokines in mouse-bearing EMT6 tumour models. IOS Press 2023-09-05 Article PeerReviewed Hasan, Nurhaslina and Hasani, Narimah Abdul Hamid and Omar, Effat and Sham, Fatihah Ronny and Fuad, Syed Baharom Syed Ahmad and Abdul Karim, Muhammad Khalis and Ibahim, Mohammad Johari (2023) A single targeted gamma-ray irradiation induced an acute modulation of immune cells and related cytokines in EMT6 mouse-bearing tumour model. Cancer Biomarkers, 38 (1). 61 - 75. ISSN 1574-0153; ESSN: 1875-8592 https://content.iospress.com/articles/cancer-biomarkers/cbm220268 10.3233/cbm-220268
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
description	BACKGROUND: A complicated interplay between radiation doses, tumour microenvironment (TME), and host immune system is linked to the active participation of immune response. OBJECTIVE: The effects of single targeted 2 Gy and 8 Gy gamma-ray irradiations on the immune cell population (lymphocytes, B-cells, T-cells, neutrophils, eosinophils, and macrophages) in EMT6 mouse-bearing tumour models was investigated. METHODS: The effects of both irradiation doses in early (96 hours) and acute phase (5 to 11 days) post-irradiation on immune parameters were monitored in blood circulation and TME using flow cytometry. Simultaneously, selected cytokines related to immune cells within the TME were measured using multiplex ELISA. RESULTS: A temporary reduction in systemic total white blood count (TWBC) resulted from an early phase (96 hours) of gamma-ray irradiation at 2 Gy and 8 Gy compared to sham control group. No difference was obtained in the acute phase. Neutrophils dominated among other immune cells in TME in sham control group. Eosinophils in TME was significantly increased after 8 Gy treatment in acute phase compared to sham control (p< 0.005). Furthermore, the increment of tumour necrosis (TNF)-±, eotaxin and interleukin (IL)-7 (p< 0.05) in both treatment groups and phases were associated with anti-tumour activities within TME by gamma-ray irradiation. CONCLUSION: The temporary changes in immune cell populations within systemic circulation and TME induced by different doses of gamma-ray irradiation correlated with suppression of several pro-tumorigenic cytokines in mouse-bearing EMT6 tumour models.
format	Article
author	Hasan, Nurhaslina Hasani, Narimah Abdul Hamid Omar, Effat Sham, Fatihah Ronny Fuad, Syed Baharom Syed Ahmad Abdul Karim, Muhammad Khalis Ibahim, Mohammad Johari
spellingShingle	Hasan, Nurhaslina Hasani, Narimah Abdul Hamid Omar, Effat Sham, Fatihah Ronny Fuad, Syed Baharom Syed Ahmad Abdul Karim, Muhammad Khalis Ibahim, Mohammad Johari A single targeted gamma-ray irradiation induced an acute modulation of immune cells and related cytokines in EMT6 mouse-bearing tumour model
author_facet	Hasan, Nurhaslina Hasani, Narimah Abdul Hamid Omar, Effat Sham, Fatihah Ronny Fuad, Syed Baharom Syed Ahmad Abdul Karim, Muhammad Khalis Ibahim, Mohammad Johari
author_sort	Hasan, Nurhaslina
title	A single targeted gamma-ray irradiation induced an acute modulation of immune cells and related cytokines in EMT6 mouse-bearing tumour model
title_short	A single targeted gamma-ray irradiation induced an acute modulation of immune cells and related cytokines in EMT6 mouse-bearing tumour model
title_full	A single targeted gamma-ray irradiation induced an acute modulation of immune cells and related cytokines in EMT6 mouse-bearing tumour model
title_fullStr	A single targeted gamma-ray irradiation induced an acute modulation of immune cells and related cytokines in EMT6 mouse-bearing tumour model
title_full_unstemmed	A single targeted gamma-ray irradiation induced an acute modulation of immune cells and related cytokines in EMT6 mouse-bearing tumour model
title_sort	single targeted gamma-ray irradiation induced an acute modulation of immune cells and related cytokines in emt6 mouse-bearing tumour model
publisher	IOS Press
publishDate	2023
url	http://psasir.upm.edu.my/id/eprint/106714/ https://content.iospress.com/articles/cancer-biomarkers/cbm220268
_version_	1811685948502573056
score	13.211869

A single targeted gamma-ray irradiation induced an acute modulation of immune cells and related cytokines in EMT6 mouse-bearing tumour model

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