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Antidiabetic and antioxidative effects of Lepisanthes fruticosa fruit seed extract in type 2 diabetic experimental rats

Lepisanthes fruticosa (Roxb) Leenh or locally known as ceri Terengganu is an underutilised fruit species from the Sapindaceae family. The species was previously identified as a potent antioxidant source, but scientific information is still lacking and limited to in vitro. Therefore, the present s...

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Main Authors: A.H.S., Mirfat, I., Amin, M.A., Mohd Shukri, H., Muhajir, K., Nur Kartinee, M., Syahida, M., Razali
Format: Article
Language:English
Published: Rynnye Lyan Resources 2023
Online Access:http://psasir.upm.edu.my/id/eprint/106614/1/106614.pdf
http://psasir.upm.edu.my/id/eprint/106614/
https://www.myfoodresearch.com/uploads/8/4/8/5/84855864/_24__fr-nfts-016_mirfat.pdf
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spelling my.upm.eprints.1066142024-09-25T04:19:15Z http://psasir.upm.edu.my/id/eprint/106614/ Antidiabetic and antioxidative effects of Lepisanthes fruticosa fruit seed extract in type 2 diabetic experimental rats A.H.S., Mirfat I., Amin M.A., Mohd Shukri H., Muhajir K., Nur Kartinee M., Syahida M., Razali Lepisanthes fruticosa (Roxb) Leenh or locally known as ceri Terengganu is an underutilised fruit species from the Sapindaceae family. The species was previously identified as a potent antioxidant source, but scientific information is still lacking and limited to in vitro. Therefore, the present study aimed to investigate the in vivo antidiabetic and antioxidative effects of L. fruticosa fruit seed extract (LFSE) in a high-fat diet, streptozotocin (HFD/STZ)-induced Sprague Dawley rats. After 8 weeks of HFD feeding, a low-dose STZ (35 mg/kg body weight) was injected to induce type 2 diabetes mellitus (T2DM). Two dosages of LFSE (300 and 600 mg/kg body weight) were orally administered to diabetic rats for 4 weeks. High dosage LFSE (600 mg/kg body weight) showed a more pronounced effect in anti-hyperglycemic activities as indicated by both sub-chronic and acute studies. The glucose-lowering ability of LFSE was supported by its improved serum insulin level (40%) as compared to diabetic control. LFSE (600 mg/kg) also resulted in a significant (P<0.05) increase in superoxide dismutase (SOD) (20%) and catalase (CAT) (43%) activities, with a significant (P<0.05) decrease in lipid peroxidation (28%) as compared to diabetic control. With regard to lipid profile, low dosage LFSE (300 mg/kg) led to a significant (P<0.05) improvement in high-density lipoprotein cholesterol (HDL-c) (39%). Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis of LFSE showed the presence of various phytochemicals with antidiabetic and antioxidant properties, with α-kojibiose, genistein-7,4'-di-O-β-D-glucoside and soyacerebroside II being the predominant ones. The findings may suggest that L. fruticosa fruit seed extract is potential in reducing hyperglycaemia and enhancing antioxidant status in HFD/STZ-induced diabetic rats. Therefore, it can be concluded that L. fruticosa fruit species may be considered as a promising candidate for a new and safe alternative remedy for the prevention and treatment of T2DM. Rynnye Lyan Resources 2023 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/106614/1/106614.pdf A.H.S., Mirfat and I., Amin and M.A., Mohd Shukri and H., Muhajir and K., Nur Kartinee and M., Syahida and M., Razali (2023) Antidiabetic and antioxidative effects of Lepisanthes fruticosa fruit seed extract in type 2 diabetic experimental rats. Food Research, 6 (suppl.2). pp. 191-201. ISSN 2550-2166 https://www.myfoodresearch.com/uploads/8/4/8/5/84855864/_24__fr-nfts-016_mirfat.pdf 10.26656/fr.2017.6(s2).016
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Lepisanthes fruticosa (Roxb) Leenh or locally known as ceri Terengganu is an underutilised fruit species from the Sapindaceae family. The species was previously identified as a potent antioxidant source, but scientific information is still lacking and limited to in vitro. Therefore, the present study aimed to investigate the in vivo antidiabetic and antioxidative effects of L. fruticosa fruit seed extract (LFSE) in a high-fat diet, streptozotocin (HFD/STZ)-induced Sprague Dawley rats. After 8 weeks of HFD feeding, a low-dose STZ (35 mg/kg body weight) was injected to induce type 2 diabetes mellitus (T2DM). Two dosages of LFSE (300 and 600 mg/kg body weight) were orally administered to diabetic rats for 4 weeks. High dosage LFSE (600 mg/kg body weight) showed a more pronounced effect in anti-hyperglycemic activities as indicated by both sub-chronic and acute studies. The glucose-lowering ability of LFSE was supported by its improved serum insulin level (40%) as compared to diabetic control. LFSE (600 mg/kg) also resulted in a significant (P<0.05) increase in superoxide dismutase (SOD) (20%) and catalase (CAT) (43%) activities, with a significant (P<0.05) decrease in lipid peroxidation (28%) as compared to diabetic control. With regard to lipid profile, low dosage LFSE (300 mg/kg) led to a significant (P<0.05) improvement in high-density lipoprotein cholesterol (HDL-c) (39%). Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis of LFSE showed the presence of various phytochemicals with antidiabetic and antioxidant properties, with α-kojibiose, genistein-7,4'-di-O-β-D-glucoside and soyacerebroside II being the predominant ones. The findings may suggest that L. fruticosa fruit seed extract is potential in reducing hyperglycaemia and enhancing antioxidant status in HFD/STZ-induced diabetic rats. Therefore, it can be concluded that L. fruticosa fruit species may be considered as a promising candidate for a new and safe alternative remedy for the prevention and treatment of T2DM.
format Article
author A.H.S., Mirfat
I., Amin
M.A., Mohd Shukri
H., Muhajir
K., Nur Kartinee
M., Syahida
M., Razali
spellingShingle A.H.S., Mirfat
I., Amin
M.A., Mohd Shukri
H., Muhajir
K., Nur Kartinee
M., Syahida
M., Razali
Antidiabetic and antioxidative effects of Lepisanthes fruticosa fruit seed extract in type 2 diabetic experimental rats
author_facet A.H.S., Mirfat
I., Amin
M.A., Mohd Shukri
H., Muhajir
K., Nur Kartinee
M., Syahida
M., Razali
author_sort A.H.S., Mirfat
title Antidiabetic and antioxidative effects of Lepisanthes fruticosa fruit seed extract in type 2 diabetic experimental rats
title_short Antidiabetic and antioxidative effects of Lepisanthes fruticosa fruit seed extract in type 2 diabetic experimental rats
title_full Antidiabetic and antioxidative effects of Lepisanthes fruticosa fruit seed extract in type 2 diabetic experimental rats
title_fullStr Antidiabetic and antioxidative effects of Lepisanthes fruticosa fruit seed extract in type 2 diabetic experimental rats
title_full_unstemmed Antidiabetic and antioxidative effects of Lepisanthes fruticosa fruit seed extract in type 2 diabetic experimental rats
title_sort antidiabetic and antioxidative effects of lepisanthes fruticosa fruit seed extract in type 2 diabetic experimental rats
publisher Rynnye Lyan Resources
publishDate 2023
url http://psasir.upm.edu.my/id/eprint/106614/1/106614.pdf
http://psasir.upm.edu.my/id/eprint/106614/
https://www.myfoodresearch.com/uploads/8/4/8/5/84855864/_24__fr-nfts-016_mirfat.pdf
_version_ 1811685944782225408
score 13.211869

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